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HUANG Shi-Rong, MENG Xiao-Lin, XU Jin-Ping, LU Wei, WANG Jian. Expression and Biological Assay of HrpN in Pichia pastoris[J]. Plant Science Journal, 2005, 23(4): 376-380.
Citation: HUANG Shi-Rong, MENG Xiao-Lin, XU Jin-Ping, LU Wei, WANG Jian. Expression and Biological Assay of HrpN in Pichia pastoris[J]. Plant Science Journal, 2005, 23(4): 376-380.
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Expression and Biological Assay of HrpN in Pichia pastoris

  • Institute of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China

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  • Received Date: November 24, 2004
  • Revised Date: June 07, 2005
  • Published Date: September 25, 2005
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    • Abstract
  • HrpN gene was placed under the control of AOX1 promoter in a Pichia expression vector pPIC6αA. Through electroporation transformation of X-33 Pichia and Blasticidin selection, a strain of Pichia pastoris capable of secreting harpinEa into the medium at high level was obtained. After initially purified, SDS-PAGE analysis of the expression products indicated that harpinEa was secreted at high level when the Pichia pastoris expression clone had been induced for 96 hours. The results of bioassay showed that the recombinant harpinEa had the ability of eliciting hypersensitive response in tobacco. The activity of this harpinEa is higher than that of harpinEa expressed in Escherichia coli.
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