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TANG Xi, XIE Dong-Li, LIU Xiao, JIANG Ji-Mou, LIANG Wen-Yu, CHEN Wei. cDNA-AFLP Analysis on Seed Abortion in Longan(Dimocarpus longan Lour.)[J]. Plant Science Journal, 2011, 29(3): 347-351.
Citation: TANG Xi, XIE Dong-Li, LIU Xiao, JIANG Ji-Mou, LIANG Wen-Yu, CHEN Wei. cDNA-AFLP Analysis on Seed Abortion in Longan(Dimocarpus longan Lour.)[J]. Plant Science Journal, 2011, 29(3): 347-351.
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cDNA-AFLP Analysis on Seed Abortion in Longan(Dimocarpus longan Lour.)

  • 1.

    Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China

  • 2.

    College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

  • 3.

    Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China

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  • Received Date: August 08, 2010
  • Revised Date: October 08, 2010
  • Published Date: June 27, 2011
    Graphical Abstract
    • Abstract
  • cDNA-AFLP technology was applied to identify differentially expressed genes between normal and abortive longan(Dimocarpus longan Lour.) seeds.Transcript-derived fragments(TDFs) identified as differentially expressed genes were eluted,cloned,sequenced and compared with the database using BLAST at NCBI.The results showed that among 11 sequenced fragments,three TDFs presented similarity with Ca2+-ATPase,cellulase,and plastidic ATP/ADP transporter,respectively,whose up-regulation or down-regulation expression might influence several important metabolic pathways in developing seeds and led to seed abortion.Four TDFs were similar with hypothetical protein,while the other four TDFs did not match with known proteins in the protein database,while sequence alignment from the DNA database also showed higher E-values,indicating they were non-homologous genes.
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