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HAO Hao-Yong, WEI Ya-Hui, WANG Ya-Ning, SUN Jie, YIN Cui-Cui. Callus Induction and Establishment of Efficient Regeneration System of Arachis hypogaea L.[J]. Plant Science Journal, 2007, 25(4): 410-412.
Citation: HAO Hao-Yong, WEI Ya-Hui, WANG Ya-Ning, SUN Jie, YIN Cui-Cui. Callus Induction and Establishment of Efficient Regeneration System of Arachis hypogaea L.[J]. Plant Science Journal, 2007, 25(4): 410-412.
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Callus Induction and Establishment of Efficient Regeneration System of Arachis hypogaea L.

  • Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, China

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  • Received Date: February 04, 2007
  • Revised Date: April 26, 2007
  • Published Date: August 27, 2007
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  • The tissue culture of peanut(Arachis hypogaea L.) was studied.The hypocotyls were used as explants on basal MS or 1/2 MS medium supplemented with different hormone combinations and agar.The result showed that the best medium for the inducement of callus was MS+6-BA 3.0 mg·L-1+NAA 0.4 mg·L-1+0.7% agar.Increasing of concentration of agar could inhibit vetrification and browning,and make callus easier to differentiate buds.The medium with 1/2 MS+NAA 1.0 mg·L-1+0.7% agar was favorable to the growth of roots and the rate of rooting was more than 95%.
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