Studies on Transgenes 1Dx5 and 1Ax1 Transferred into Elite Wheat Cultivars by Cross Breeding

For introducing the transgenes from transgenic wheat ’B72-8-11b’ and ’B102-1-2’ into elite wheat lines,two wheat crosses had been made in 2002.In the crosses,elite subunits 1Dx5 and 1Ax1 were over-expressed in the male parents ’B72-8-11b’ and ’B102-1-2’ respectively,while the female parent ’Emai 12’ were selected with better agronomic traits.The expression of exogenous 1Dx5 and 1Ax1 gene in F₁,F₂,F₃ and F₄ generations was tested by SDS-PAGE,then the inheritance patten of the transgenes was analysed.Two lines had been selected from the progenies of the crosses in which the subunit 1Dx5 or 1Ax1 is over-expressed and the endogenous HMW-GS compositions are different from each other.The results demonstrated that the introduction of transgenes from transgenic wheat lines into elite varieties by crossing is an effective way to transfer extra HMW-GS gene.