2009 Vol. 27 No. 2
2009, 27(2): 121-126.
Abstract:
The chromosome number and karyotype of six species in Cleisostoma from Hainan were studied by applying root tip squash method.The results showed that the Cleisostoma paniculatum(ker-Gawl.) Garay 2n=2x=38=36m+2sm,C.striatum(Rchb.f.) Garay 2n=2x=38=2M+36m,C.parishii(Hook.f.) Garay 2n=2x=38=2M+34m+2sm,C.filiforme(Lindl.) Garay 2n=2x=38=38m,C.rostratum(Lodd.) Seidenf 2n=2x=38=36m+2sm and C.williamsonii(Rchb.f.) Garay 2n=2x=38=34m+4sm(2SAT).Its karyotype mainly consists of metacentric chromosomes.All of the karyotypes belong to 1B type except the C.rostratum(Lodd.) Seidenf is 1A.
The chromosome number and karyotype of six species in Cleisostoma from Hainan were studied by applying root tip squash method.The results showed that the Cleisostoma paniculatum(ker-Gawl.) Garay 2n=2x=38=36m+2sm,C.striatum(Rchb.f.) Garay 2n=2x=38=2M+36m,C.parishii(Hook.f.) Garay 2n=2x=38=2M+34m+2sm,C.filiforme(Lindl.) Garay 2n=2x=38=38m,C.rostratum(Lodd.) Seidenf 2n=2x=38=36m+2sm and C.williamsonii(Rchb.f.) Garay 2n=2x=38=34m+4sm(2SAT).Its karyotype mainly consists of metacentric chromosomes.All of the karyotypes belong to 1B type except the C.rostratum(Lodd.) Seidenf is 1A.
2009, 27(2): 127-132.
Abstract:
Chromosome number and karyomorphology of seven species from section Proliferae of Primula(Primulaceae) were investigated.The karyomorphology of two species:P.chrysochlora Balf.f.et Ward and P.mallophylla Balf.f.were reported for the first time.By analysing the data in the seven species and available data published in this section,the result suggested that most species showed far-reaching conformity in size,shape of the chromosome and the main arrangement of the constrictions.The difference of karyomorphology between species was small,and most species were of median-centromeric and submedian-centromeric,which belonged to Stebbins’ 2A or 2B.The basic chromosome number is x=11,which supported that sect.Proliferae was close to some sections with same basic chromosome number in Primula.To offer cytology proof for systematic evolution in section Proliferae,we also combined the published data of cytology and morphological feature in section Proliferae for comparative analysis.
Chromosome number and karyomorphology of seven species from section Proliferae of Primula(Primulaceae) were investigated.The karyomorphology of two species:P.chrysochlora Balf.f.et Ward and P.mallophylla Balf.f.were reported for the first time.By analysing the data in the seven species and available data published in this section,the result suggested that most species showed far-reaching conformity in size,shape of the chromosome and the main arrangement of the constrictions.The difference of karyomorphology between species was small,and most species were of median-centromeric and submedian-centromeric,which belonged to Stebbins’ 2A or 2B.The basic chromosome number is x=11,which supported that sect.Proliferae was close to some sections with same basic chromosome number in Primula.To offer cytology proof for systematic evolution in section Proliferae,we also combined the published data of cytology and morphological feature in section Proliferae for comparative analysis.
2009, 27(2): 133-144.
Abstract:
Leaf epidermis of 13 species in the genus Bupleurum L.was examined under both light microscope(LM) and scanning electron microscope(SEM).Stomatas of most species occur in both abaxial and adaxial epidermis,and only B.longiradiatum Turczaninow has stomatas in abaxial epidermis.The shape of epidermal cells in Bupleurum examined are generally polygonal,and the anticlinal cell walls are either straight or curved.Only a few species are irregular,with the anticlinal cell walls sinuolate.Guard cells have thickened walls,forming polar lamellae at the polar end.Under the SEM,the cuticular membrane in the genus is usually striated.The wax ornamentations are flaked,striate to wrinkled or granular.These uniform micromorphological characteristics in the genus are important in classifications of studied species.
Leaf epidermis of 13 species in the genus Bupleurum L.was examined under both light microscope(LM) and scanning electron microscope(SEM).Stomatas of most species occur in both abaxial and adaxial epidermis,and only B.longiradiatum Turczaninow has stomatas in abaxial epidermis.The shape of epidermal cells in Bupleurum examined are generally polygonal,and the anticlinal cell walls are either straight or curved.Only a few species are irregular,with the anticlinal cell walls sinuolate.Guard cells have thickened walls,forming polar lamellae at the polar end.Under the SEM,the cuticular membrane in the genus is usually striated.The wax ornamentations are flaked,striate to wrinkled or granular.These uniform micromorphological characteristics in the genus are important in classifications of studied species.
2009, 27(2): 145-151.
Abstract:
By using inter-simple sequence(ISSR) markers,this paper studied the genetic diversity and clonal structure of two Heleocharis valleculosa f.setosa populations(120 individuals) in Poyang Lake.By five primers,85 fragments were amplified,of which 49(57.65%) were polymorphic.As a result,a relatively high level of genetic diversity was revealed(H=0.1545,I=0.2400,at species level),but a low level of genetic differentiation was detected between the two populations(GST=0.0201).High clonal diversity was detected but no common genotype found between the two populations,which meant great clonal differentiation.In both populations,the spatial genetic structure were found as a random mode.
By using inter-simple sequence(ISSR) markers,this paper studied the genetic diversity and clonal structure of two Heleocharis valleculosa f.setosa populations(120 individuals) in Poyang Lake.By five primers,85 fragments were amplified,of which 49(57.65%) were polymorphic.As a result,a relatively high level of genetic diversity was revealed(H=0.1545,I=0.2400,at species level),but a low level of genetic differentiation was detected between the two populations(GST=0.0201).High clonal diversity was detected but no common genotype found between the two populations,which meant great clonal differentiation.In both populations,the spatial genetic structure were found as a random mode.
2009, 27(2): 152-158.
Abstract:
Located in the north-edge of tropical Asia,the flora of Hainan Island has ancient tropical floristic compositions as well as ancient subtropical floristic compositions of China,due to its diversity of natural landforms and climate.In this paper,we chose coastal and hilly areas such as Liudaoling Nature Reserve,Huoling Nature Reserve,Luhuitouling Mountain,Nanshanling Mountain and Hongxialing Mountain(which all locate in the southern Island and are called QN below),and Tongguling Nature Reserve(which locates in the northern Island and is called QB below) as research areas.After surveying the plots,we obtained enough data and compared floristic compositions of two areas.Results show that floristic compositions of both areas had similarities and differences:In QN,there are 876 species of spermatophyte,belonging to 112 families and 484 genera.Geographic elements include tropical ones,temperate ones and Chinese endemic ones.Tropical elements are dominant and Hainan endemic ones are rich,which shows it is transitional elements;In QB,there are 934 species of spermatophyte,which belong to 141 families,580 genera.Geographic elements,which include tropical ones,temperate ones and endemic ones,are dominated by tropical elements and rich in Hainan endemic elements.The common characteristics of flora components of two areas are that plant species are abundant,geographic elements are diversified,dominant elements are significant and proportion of tropical elements is high.
Located in the north-edge of tropical Asia,the flora of Hainan Island has ancient tropical floristic compositions as well as ancient subtropical floristic compositions of China,due to its diversity of natural landforms and climate.In this paper,we chose coastal and hilly areas such as Liudaoling Nature Reserve,Huoling Nature Reserve,Luhuitouling Mountain,Nanshanling Mountain and Hongxialing Mountain(which all locate in the southern Island and are called QN below),and Tongguling Nature Reserve(which locates in the northern Island and is called QB below) as research areas.After surveying the plots,we obtained enough data and compared floristic compositions of two areas.Results show that floristic compositions of both areas had similarities and differences:In QN,there are 876 species of spermatophyte,belonging to 112 families and 484 genera.Geographic elements include tropical ones,temperate ones and Chinese endemic ones.Tropical elements are dominant and Hainan endemic ones are rich,which shows it is transitional elements;In QB,there are 934 species of spermatophyte,which belong to 141 families,580 genera.Geographic elements,which include tropical ones,temperate ones and endemic ones,are dominated by tropical elements and rich in Hainan endemic elements.The common characteristics of flora components of two areas are that plant species are abundant,geographic elements are diversified,dominant elements are significant and proportion of tropical elements is high.
2009, 27(2): 159-164.
Abstract:
The management and control of invasive alien plants are primarily based on the knowledge of their spatial patterns and the major factors.In this study,the spatial patterns of floristic composition of genus of invasive alien plants in China and their climatic interpretation were investigated based on information from literature and climate data.The results showed that tropical elements dominated the study area,followed by temperate elements and cosmopolitan elements,respectively.From south to north,there was a decreasing trend in the ratios of tropical elements,while an increasing trend was observed in the ratios of temperate elements.It indicated that in southern region,invasive alien plants mainly came from more tro-pical regions,while in northern region plants from temperate area gained the domination.Energy strongly shaped the spatial patterns of floristic composition.With the increase of mean annual temperature,tropical elements showed an increasing trend,while a decreasing trend was observed in temperate elements,which may be due to their different ecological characters.
The management and control of invasive alien plants are primarily based on the knowledge of their spatial patterns and the major factors.In this study,the spatial patterns of floristic composition of genus of invasive alien plants in China and their climatic interpretation were investigated based on information from literature and climate data.The results showed that tropical elements dominated the study area,followed by temperate elements and cosmopolitan elements,respectively.From south to north,there was a decreasing trend in the ratios of tropical elements,while an increasing trend was observed in the ratios of temperate elements.It indicated that in southern region,invasive alien plants mainly came from more tro-pical regions,while in northern region plants from temperate area gained the domination.Energy strongly shaped the spatial patterns of floristic composition.With the increase of mean annual temperature,tropical elements showed an increasing trend,while a decreasing trend was observed in temperate elements,which may be due to their different ecological characters.
2009, 27(2): 165-170.
Abstract:
Lurix priucipis-rupprechtii is one of main constructive tree species of conifer forest which is dis-tributed in semi-humid region,north China. The population structure and spatial pattern of different size classes were studied in a natural regeneration stand which lies in a clear-cut strip. The results revealed that Larix principis-rupprechtii population trends decline for the shortage of seedling and sapling. But the population has the potential to be stable in respect that long life span,abundant trees of middle size class and disturbance. The seedlings and saplings were clumped. Trees of 2. 5 cm≤>Larix principis-rupprechtii was related to itself biological characteristics and light.
Lurix priucipis-rupprechtii is one of main constructive tree species of conifer forest which is dis-tributed in semi-humid region,north China. The population structure and spatial pattern of different size classes were studied in a natural regeneration stand which lies in a clear-cut strip. The results revealed that Larix principis-rupprechtii population trends decline for the shortage of seedling and sapling. But the population has the potential to be stable in respect that long life span,abundant trees of middle size class and disturbance. The seedlings and saplings were clumped. Trees of 2. 5 cm
2009, 27(2): 171-175.
Abstract:
Based on plot survey data at different slope positions,the dynamics of Helianthemum ordosicum population were analyzed using the spectral analysis method.Results showed that the natural regeneration dynamics of H.ordosicum population were presented as fluctuation of the distribution of individuals by age classes determined from the ground diameter.The quantitative dynamics of H.ordosicum population at different slope positions were greatly affected by the base wave.The apparent mid-and small-scale periodic fluctuations also existed.The mid-scale periodic fluctuations were about half of the base waves in the 12 mm-15 mm increment of ground diameter,and the small-scale periodic fluctuations were reflected in the 2.00 mm-6.25 mm increment of ground diameter regardless of slope positions.The mid-and small-scale periodic fluctuations were related to the environment pressure,interspecific competition and the intrinsic physiological characteristics.Because of the different habitat conditions,the small-scale periodism in the increment of ground diameter decreased from the lower slope position to the upper slope position in H.ordosicum population.Human disturbance also affected the periodic fluctuations.The periodic fluctuations of the quantitative dynamics maintained and sustained the self-stability in H.ordosicum populations.
Based on plot survey data at different slope positions,the dynamics of Helianthemum ordosicum population were analyzed using the spectral analysis method.Results showed that the natural regeneration dynamics of H.ordosicum population were presented as fluctuation of the distribution of individuals by age classes determined from the ground diameter.The quantitative dynamics of H.ordosicum population at different slope positions were greatly affected by the base wave.The apparent mid-and small-scale periodic fluctuations also existed.The mid-scale periodic fluctuations were about half of the base waves in the 12 mm-15 mm increment of ground diameter,and the small-scale periodic fluctuations were reflected in the 2.00 mm-6.25 mm increment of ground diameter regardless of slope positions.The mid-and small-scale periodic fluctuations were related to the environment pressure,interspecific competition and the intrinsic physiological characteristics.Because of the different habitat conditions,the small-scale periodism in the increment of ground diameter decreased from the lower slope position to the upper slope position in H.ordosicum population.Human disturbance also affected the periodic fluctuations.The periodic fluctuations of the quantitative dynamics maintained and sustained the self-stability in H.ordosicum populations.
2009, 27(2): 176-183.
Abstract:
The toxic effects of environmental Cd2+ pollution on Sagittaria sagittifolia L.were mainly stu-died.It’s found that Cd2+ toxicity induced the roots different degree of browning,blackening and growth restriction.The ultrastructural damages of the leaf and root cells were mainly expressed in chloroplast swelled,thylakoids disordered,mitochondrial cristae decreased,mitochondria swelled and vacuoled as well as chromatin polymerized.The cell wall incrassated,cell shape twisted and cell arrange disordered in the scanning electron microscope.The energy spectrum analysis showed that with the environmental Cd2+ concentration increasing,the Cd2+ contents in a single root tip increased,and the absorption of other nutrients was affected to varying degrees.Cadmium toxicity on S.sagittifolia L.had clear dose-response and time effects.
The toxic effects of environmental Cd2+ pollution on Sagittaria sagittifolia L.were mainly stu-died.It’s found that Cd2+ toxicity induced the roots different degree of browning,blackening and growth restriction.The ultrastructural damages of the leaf and root cells were mainly expressed in chloroplast swelled,thylakoids disordered,mitochondrial cristae decreased,mitochondria swelled and vacuoled as well as chromatin polymerized.The cell wall incrassated,cell shape twisted and cell arrange disordered in the scanning electron microscope.The energy spectrum analysis showed that with the environmental Cd2+ concentration increasing,the Cd2+ contents in a single root tip increased,and the absorption of other nutrients was affected to varying degrees.Cadmium toxicity on S.sagittifolia L.had clear dose-response and time effects.
2009, 27(2): 184-187.
Abstract:
Air-dried leaves of Epimedium in the nursery were collected at the full-bloom stage,and then investigated the contents of four different pharmacological flavonoids by high-performance liquid chromatography.Leaf area,leaf length,and leaf width were measured before processing materials.The results show that there is significant correlation between the leaf morphology of Epimedium species and the contents of flavonoids:leaves with high flavonoid content,are usually oval or long oval shape and less leathery.
Air-dried leaves of Epimedium in the nursery were collected at the full-bloom stage,and then investigated the contents of four different pharmacological flavonoids by high-performance liquid chromatography.Leaf area,leaf length,and leaf width were measured before processing materials.The results show that there is significant correlation between the leaf morphology of Epimedium species and the contents of flavonoids:leaves with high flavonoid content,are usually oval or long oval shape and less leathery.
2009, 27(2): 188-192.
Abstract:
DRE cis-acting element which could specifically bind DREB transcription factors plays important roles in inducing gene expression involved in dehydration,low-temperature,and high-salinity stresses.Double-stranded DNA microarrys could very effectively detect the interaction between DNA-cis-acting elements and protein-transcription factors in regulation of gene expression,and also could availably analyze the binding interaction of bio-molecular.In this study,we designed and chemically synthesized a hairpin structure single-stranded DNA probe according to the nuclear sequence of DRE cis-acting element.Furthermore,we adopted Taq DNA polymerase in the elongation system and optimized the new on-chip reaction conditions.Our experiments showed that the elongation temperature of 50℃,the Mg2+ concentration of 2.5 mmol/L and single-stranded,non-denatured probe are the optimal conditions in elongation with Taq DNA polymerase.These results provide further steps toward a better understanding of interaction mechanisms between DRE cis-acting element and DREB transcription factors.
DRE cis-acting element which could specifically bind DREB transcription factors plays important roles in inducing gene expression involved in dehydration,low-temperature,and high-salinity stresses.Double-stranded DNA microarrys could very effectively detect the interaction between DNA-cis-acting elements and protein-transcription factors in regulation of gene expression,and also could availably analyze the binding interaction of bio-molecular.In this study,we designed and chemically synthesized a hairpin structure single-stranded DNA probe according to the nuclear sequence of DRE cis-acting element.Furthermore,we adopted Taq DNA polymerase in the elongation system and optimized the new on-chip reaction conditions.Our experiments showed that the elongation temperature of 50℃,the Mg2+ concentration of 2.5 mmol/L and single-stranded,non-denatured probe are the optimal conditions in elongation with Taq DNA polymerase.These results provide further steps toward a better understanding of interaction mechanisms between DRE cis-acting element and DREB transcription factors.
2009, 27(2): 193-196.
Abstract:
In this paper,the orthogonal design was used to optimize DDRT-PCR amplification system on Racomitrium japonicum in five levels of six factors(Mg2+,dNTP,anchor primer,random primer,DNA template,Taq DNA polymerase) respectively.We established a most suitable DDRT-PCR system for R.japonicum,which contains Mg2+ 2.25 mmol/L,dNTP 0.4 mmol/L,anchor primer 1.0 μmol/L,random primer 0.7 μmol/L,DNA template 1.6 μL,and Taq DNA polymerase 2.5 U in 20 μL reaction system.At the last,the optimal annealing temperature for DDRT-PCR reaction was proposed by gradient PCR and it’s 45.4℃.The result provided an important reference for the selecting and cloning of drought-resistant genes of R.japonicum.
In this paper,the orthogonal design was used to optimize DDRT-PCR amplification system on Racomitrium japonicum in five levels of six factors(Mg2+,dNTP,anchor primer,random primer,DNA template,Taq DNA polymerase) respectively.We established a most suitable DDRT-PCR system for R.japonicum,which contains Mg2+ 2.25 mmol/L,dNTP 0.4 mmol/L,anchor primer 1.0 μmol/L,random primer 0.7 μmol/L,DNA template 1.6 μL,and Taq DNA polymerase 2.5 U in 20 μL reaction system.At the last,the optimal annealing temperature for DDRT-PCR reaction was proposed by gradient PCR and it’s 45.4℃.The result provided an important reference for the selecting and cloning of drought-resistant genes of R.japonicum.
2009, 27(2): 197-203.
Abstract:
Parameters and indexes derived from different models of photosynthesis-light response showed distant values,but these models were still the major methods to judge the feature of plant photosynthesis so far.In this paper,four light response models including the rectangle hyperbola,the non-rectangle hyperbola and two kinds of index curves were used to fit the light response data of evergreen poplar clone.Parameters and indexes from different models were compared.Methods of calculating light saturation point(LSP) were also studied in this paper.Results showed that the main characteristic parameters,including maximum net photosynthetic rate(P’max),apparent quantum efficiency(a),dark respiration rate(Rd),which were obtained from the models of hyperbola,were higher than those from two index curve models.And parameters estimated from rectangle hyperbola were higher than those of the non-rectangle hyperbola.While the parameters derived from two kinds of index models appeared no difference.As for calculation methods of LSP,the LSP obtained from the straight line fitted by photosynthetic photon flux density under 200 μmo·lm-2·s-1 combined with other models was underestimated and this method seems unreliable.It is also unreasonable that LSP was estimated through the maximum gross photosynthesis rate(Pmax).However,LSP could be effectively estimated through P’max by eliminating the influence of Rd,and the estimation percentage of each models was basically fixed.The percentages for calculating LSP through P’max in rectangle hyperbola,non-rectangle hyperbola and index curves were suggested to be(78±1)%,(82±1)% and(96±1)%,respectively.Beside,light response data of Eucalyptus grandis×E.urophylla were used to validate those conclusions.
Parameters and indexes derived from different models of photosynthesis-light response showed distant values,but these models were still the major methods to judge the feature of plant photosynthesis so far.In this paper,four light response models including the rectangle hyperbola,the non-rectangle hyperbola and two kinds of index curves were used to fit the light response data of evergreen poplar clone.Parameters and indexes from different models were compared.Methods of calculating light saturation point(LSP) were also studied in this paper.Results showed that the main characteristic parameters,including maximum net photosynthetic rate(P’max),apparent quantum efficiency(a),dark respiration rate(Rd),which were obtained from the models of hyperbola,were higher than those from two index curve models.And parameters estimated from rectangle hyperbola were higher than those of the non-rectangle hyperbola.While the parameters derived from two kinds of index models appeared no difference.As for calculation methods of LSP,the LSP obtained from the straight line fitted by photosynthetic photon flux density under 200 μmo·lm-2·s-1 combined with other models was underestimated and this method seems unreliable.It is also unreasonable that LSP was estimated through the maximum gross photosynthesis rate(Pmax).However,LSP could be effectively estimated through P’max by eliminating the influence of Rd,and the estimation percentage of each models was basically fixed.The percentages for calculating LSP through P’max in rectangle hyperbola,non-rectangle hyperbola and index curves were suggested to be(78±1)%,(82±1)% and(96±1)%,respectively.Beside,light response data of Eucalyptus grandis×E.urophylla were used to validate those conclusions.
2009, 27(2): 211-215.
Abstract:
Paralogs and orthologs are two main types of the homologous sequences originated during speciation and genome evolution.Although methods of ortholog identification across species have been develo-ped,the method or parameters for paralog prediction are still uncertain especially for solanaceous species.Now whole genome sequencing of tomato is under going,parameters for paralog prediction are needed to study the evolution of tomato genome.After self-blasting of tomato BAC DNA sequences downloaded from GenBank,here we determined that E-value≤10-40,HSPs length≥200 bp,sequence identity≥80% are the appropriate parameters or combination of parameters.
Paralogs and orthologs are two main types of the homologous sequences originated during speciation and genome evolution.Although methods of ortholog identification across species have been develo-ped,the method or parameters for paralog prediction are still uncertain especially for solanaceous species.Now whole genome sequencing of tomato is under going,parameters for paralog prediction are needed to study the evolution of tomato genome.After self-blasting of tomato BAC DNA sequences downloaded from GenBank,here we determined that E-value≤10-40,HSPs length≥200 bp,sequence identity≥80% are the appropriate parameters or combination of parameters.
2009, 27(2): 216-220.
Abstract:
The information of protein subcellular localization is important to our understanding of a protein function.The proteins encoded by both At4g16410 and At1g18060 were predicted to be the Arabidopsis chloroplast proteins.The 199 bp and 220 bp fragments of this two genes at the 5’ end were cloned and fused with GFP to construct the binary vectors pMON530-cTP1-GFP and pMON530-cTP2-GFP for genetic transformation,respectively.Observed by a confocal laser-scanning microscopy,green fluorescent signals were localized in chloroplasts in transgenic Arabidopsis plants suggesting the 199 bp and 220 bp fragments encoding peptides targeted to chloroplast.The results suggest that At4g16410 and At1g18060 encode two chloroplast proteins.
The information of protein subcellular localization is important to our understanding of a protein function.The proteins encoded by both At4g16410 and At1g18060 were predicted to be the Arabidopsis chloroplast proteins.The 199 bp and 220 bp fragments of this two genes at the 5’ end were cloned and fused with GFP to construct the binary vectors pMON530-cTP1-GFP and pMON530-cTP2-GFP for genetic transformation,respectively.Observed by a confocal laser-scanning microscopy,green fluorescent signals were localized in chloroplasts in transgenic Arabidopsis plants suggesting the 199 bp and 220 bp fragments encoding peptides targeted to chloroplast.The results suggest that At4g16410 and At1g18060 encode two chloroplast proteins.
2009, 27(2): 221-224.
Abstract:
The embryo rescue technique was applied to obtain interspecific hybrids between Brassica napus and Purple-caitai.Of the media studied,MS supplemented with(1.0-2.0)mg·L-1 6-BA+0.05 mg·L-1 NAA+0.5% activated carbon+30 g·L-1 sugar+7.5 g·L-1 agar gave the most satisfactory results for ovary culture.The effect of activated carbon is more significant than that of medium and plant hormone in ovary culture.Ovaries taken 9,12,15 and 18 days after pollination were used for culture,and those taken 18 days after pollination appeared most efficient for seed production,that of 15 days takes second place.But the results by studying the germination of their hybrid seeds showed the germination rate of the ovary cultured hybrids taken 15 days after pollination is highest,namely 57.03%,the lowest in 18 days,Only 38.49%.
The embryo rescue technique was applied to obtain interspecific hybrids between Brassica napus and Purple-caitai.Of the media studied,MS supplemented with(1.0-2.0)mg·L-1 6-BA+0.05 mg·L-1 NAA+0.5% activated carbon+30 g·L-1 sugar+7.5 g·L-1 agar gave the most satisfactory results for ovary culture.The effect of activated carbon is more significant than that of medium and plant hormone in ovary culture.Ovaries taken 9,12,15 and 18 days after pollination were used for culture,and those taken 18 days after pollination appeared most efficient for seed production,that of 15 days takes second place.But the results by studying the germination of their hybrid seeds showed the germination rate of the ovary cultured hybrids taken 15 days after pollination is highest,namely 57.03%,the lowest in 18 days,Only 38.49%.
2009, 27(2): 225-231.
Abstract:
A ‘Painting Zenia insignis Chun’ drawn by Feng Jeng-Ru is studied in detail.The results indicate that the plant in the painting is Zenia insignis Chun,which was published by Chun Woon-Young in 1946 and named after a famous scholar Zen Houng-Chsun.Meanwhile,Hu Hsen-Hsu praisefully indited a poem ‘Zenia insignis Song’ in the painting.The resumes of Hu Hsen-Hsu and Chun Woon-Young,Feng Jeng-Ru,and Zen Houng-Chsun in plant sciences,respectively as the founders of modern plant taxonomy,botanical scientific illustration,and modern science in China,are introduced.Therefore,the ‘Painting Zenia insignis Chun’ vividly represents four boffins’ friendships and contributions.
A ‘Painting Zenia insignis Chun’ drawn by Feng Jeng-Ru is studied in detail.The results indicate that the plant in the painting is Zenia insignis Chun,which was published by Chun Woon-Young in 1946 and named after a famous scholar Zen Houng-Chsun.Meanwhile,Hu Hsen-Hsu praisefully indited a poem ‘Zenia insignis Song’ in the painting.The resumes of Hu Hsen-Hsu and Chun Woon-Young,Feng Jeng-Ru,and Zen Houng-Chsun in plant sciences,respectively as the founders of modern plant taxonomy,botanical scientific illustration,and modern science in China,are introduced.Therefore,the ‘Painting Zenia insignis Chun’ vividly represents four boffins’ friendships and contributions.