Selection of a Carbohydrate-rich Microalgae and Its Molecular Identification

ZHANG Hu; WEN Xiao-Bin; WANG Zhong-Jie; LI Ye-Guang; GENG Ya-Hong

doi:10.11913/PSJ.2095-0837.2014.60645

Plant Science Journal > 2014 > 32(6): 645-654. > DOI: 10.11913/PSJ.2095-0837.2014.60645 CSTR: 32231.14.PSJ.2095-0837.2014.60645

ZHANG Hu, WEN Xiao-Bin, WANG Zhong-Jie, LI Ye-Guang, GENG Ya-Hong. Selection of a Carbohydrate-rich Microalgae and Its Molecular Identification[J]. Plant Science Journal, 2014, 32(6): 645-654. DOI: 10.11913/PSJ.2095-0837.2014.60645

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Selection of a Carbohydrate-rich Microalgae and Its Molecular Identification

1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Science, Wuhan 430074, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

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Received Date: February 18, 2014
Available Online: October 31, 2022
Published Date: December 29, 2014

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Abstract

Abstract

Microalgae are an ideal raw material for bioethanol production because of fast growth and high carbohydrate productivity. Ten pre-selected microalgae strains were cultivated in an air-bubble column photobioreactor to test the characteristics of carbohydrate production, and carbohydrate productivity was used as the key parameter to select the strains suitable for carbohydrate production. Results showed that biomass, soluble sugar content, total carbohydrate content and carbohydrate productivity of the tested strains were in the range of 0.922 - 1.965 g/L, 4.42% - 19.23%, 26.8% - 60.9% and 36.17 - 149.67 mg·L^-1·d^-1, respectively. Both carbohydrate productivity (149.67 mg·L^-1·d^-1) and soluble sugar content (19.23%) of GZ-57 were the highest among the investigated strains, and GZ-57 was selected as the candidate strain with greatest potential in bioethanol production. Morphological observation and molecular phylogenetic analyses of 18S rDNA gene sequences and internal transcribed sequences (ITS) indicated that GZ-57 belonged to the Desmodesmus genus, and was identified as Desmodesmus maximus.
- Desmodesmus maximus,
- Bioethanol,
- Carbohydrate productivity,
- Molecular identification

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References (41)

References

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