DNA Barcoding of the Genus <em>Rehmannia</em> (Scrophulariaceae)

CHENG Fang-Ting; LI Zhong-Hu; LIU Chun-Yan; YUAN Chao; LI Xue-Tong; LIU Zhan-Lin

doi:10.11913/PSJ.2095-0837.2015.10025

Plant Science Journal > 2015 > 33(1): 25-32. > DOI: 10.11913/PSJ.2095-0837.2015.10025 CSTR: 32231.14.PSJ.2095-0837.2015.10025

CHENG Fang-Ting, LI Zhong-Hu, LIU Chun-Yan, YUAN Chao, LI Xue-Tong, LIU Zhan-Lin. DNA Barcoding of the Genus Rehmannia (Scrophulariaceae)[J]. Plant Science Journal, 2015, 33(1): 25-32. DOI: 10.11913/PSJ.2095-0837.2015.10025

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DNA Barcoding of the Genus Rehmannia (Scrophulariaceae)

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, China

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Received Date: April 13, 2014
Revised Date: May 14, 2014
Available Online: October 31, 2022
Published Date: February 27, 2015

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Abstract

Abstract

Rehmannia, a genus of Scrophulariaceae, is widely distributed in central, eastern and northern China. However, it is relatively difficult to distinguish species by traditional morphological methods due to rapid speciation of the genus. Recently developed DNA barcoding provides a new approach to identify species quickly and accurately. In this study, three candidate DNA noncoding regions (trnL-trnF, trnM-trnV and trnS-trnG) from the chloroplast genome and internal transcribed spacer (ITS) region from the nuclear genome were evaluated among 75 individuals of five species of Rehmannia, and identification efficiency was assessed using PWG-distance and Tree-Building methods. Results indicated that the five Rehmannia species could not be accurately distinguished by single or the combination of chloroplast DNA fragments with low species discrimination rates (0%-20%), but could by the combination of chloroplasts and nuclear DNA regions (trnS-trnG+ITS) with 100% discrimination rates. Thus, trnS-trnG+ITS can be considered as a potential barcode for species identification in Rehmannia. The combination of chloroplasts and nuclear fragments can be widely adopted for the discrimination of plant species when a single DNA barcode fails.
- DNA barcoding,
- Rehmannia,
- Chloroplast DNA,
- nrDNA ITS,
- Discrimination rate

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