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ZHANG Hong-Pei, ZHANG Xiao-Ru, HU Ge-Ge, DONG Juan-E. Relationship between H2O2 Burst and Medium Alkalinization Induced by Salicylic Acid in Salvia miltiorrhiza Cell Suspension Cultures[J]. Plant Science Journal, 2015, 33(3): 405-413. DOI: 10.11913/PSJ.2095-0837.2015.30405
Citation: ZHANG Hong-Pei, ZHANG Xiao-Ru, HU Ge-Ge, DONG Juan-E. Relationship between H2O2 Burst and Medium Alkalinization Induced by Salicylic Acid in Salvia miltiorrhiza Cell Suspension Cultures[J]. Plant Science Journal, 2015, 33(3): 405-413. DOI: 10.11913/PSJ.2095-0837.2015.30405
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Relationship between H2O2 Burst and Medium Alkalinization Induced by Salicylic Acid in Salvia miltiorrhiza Cell Suspension Cultures

  • College of Life Sciences, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China

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  • Received Date: August 12, 2014
  • Available Online: October 31, 2022
  • Published Date: June 27, 2015
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    • Abstract
  • Salicylic acid (SA) treatment can induce significant H2O2 burst in Salvia miltiorrhiza suspension culture cell and its medium alkalinization. In this study, the correlation between SA-induced H2O2 burst and medium alkalinization was investigated through NADPH oxidase inhibitor imidazole (IMD), H2O2 quenching agent dimethylthiourea (DMTU), plasma membrane H+-ATPase inhibitor Na3VO4 and plasma membrane H+-ATPase activator fusicoccin (FC). Results showed that (1) H2O2 treatment also induced significant medium alkalinization, and IMD and DMTU suppressed the process of SA-induced medium alkalinization, which suggested that H2O2 was involved in the process of SA-induced medium alkalinization; (2) SA suppressed the activity of plasma membrane H+-ATPase, Na3VO4 treatment caused rapid medium alkalinization and led to H2O2 burst ahead of time, and FC reversed SA-induced medium alkalinization and H2O2 accumulation, which suggested that plasma membrane H+-ATPase regulated the change in medium pH value and medium alkalinization promoted the generation of H2O2. Overall, the H2O2 level in the S. miltiorrhiza suspension culture cell and its medium alkalinization level functioned together to respond to induction of salicylic acid.
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