Dynamic Changes of Methylcytosine in Polarized Cell Growth and Their Response to GABA Signals

CUI Guo-Peng; WANG Sheng-Jie; QIN Rui; LIU Hong; GONG Han-Yu; LI Gang; YU Guang-Hui

doi:10.11913/PSJ.2095-0837.2015.40521

Plant Science Journal > 2015 > 33(4): 521-527. > DOI: 10.11913/PSJ.2095-0837.2015.40521 CSTR: 32231.14.PSJ.2095-0837.2015.40521

CUI Guo-Peng, WANG Sheng-Jie, QIN Rui, LIU Hong, GONG Han-Yu, LI Gang, YU Guang-Hui. Dynamic Changes of Methylcytosine in Polarized Cell Growth and Their Response to GABA Signals[J]. Plant Science Journal, 2015, 33(4): 521-527. DOI: 10.11913/PSJ.2095-0837.2015.40521

Citation:

PDF (1121 KB)

Dynamic Changes of Methylcytosine in Polarized Cell Growth and Their Response to GABA Signals

Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Engineering Research Centre for the Protection and Utilization of Bioresource in Ethnic Area of Southern China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China

More Information

Received Date: January 18, 2015
Published Date: August 27, 2015

Graphical Abstract

Abstract

Abstract

DNA cytosine methylation (5mC) plays an important role in plant development. A variety of environmental factors, such as cellular endogenous and exogenous stress factors, can trigger changes in DNA methylation. To explore the possible mechanism regulated by γ-aminobutyric acid (GABA) in plant development, we analyzed the content of 5mC and its response to GABA signals in the polarized growth of tobacco pollen tubes and Arabidopsis roots. Results showed that GABA (1.0 mmol/L) significantly promoted the growth of tobacco pollen tubes and Arabidopsis roots, significantly decreased the content of 5mC in the genomic DNA of tobacco pollen tubes and Arabidopsis roots, and increased the content of 5-hydroxyl cytosine (5hmC). 5hmC is an important intermediate of 5mC active demethylation via its oxidative metabolic pathway. Our results demonstrated that GABA, as an important exogenous signal, could regulate dynamic changes in DNA methylation.
- γ-Aminobutyric acid(GABA),
- Methylated DNA,
- 5-Methylcytosine(5mC),
- 5-Hydroxyl methylcytosine(5hmC)

FullText(HTML)

References (26)

References

[1]	Maiti A, Michelson AZ, Armwood CJ, Lee JK, Drohat AC. Divergent mechanisms for enzymatic excision of 5-formylcytosine and 5-carboxylcytosine from DNA[J].J Am Chem Soc, 2013, 135(42): 15813-15822.
[2]	Bagci H, Fisher AG. DNA demethylation in pluripotency and reprogramming: the role of tet proteins and cell division[J].Cell Stem Cell, 2013, 13(3): 265-269.
[3]	Wang L, Zhang J, Duan J, Gao X, Zhu W, Lu X, Yang L, Zhang J, Li G, Ci W, Li W, Zhou Q, Aluru N, Tang F, He C, Huang X, Liu J. Programming and inheritance of parental DNA methylomes in mammals[J].Cell, 2014, 157(4): 979-991.
[4]	Kawashima T, Berger F. Epigenetic reprogramming in plant sexual reproduction[J].Nat Rev Genet, 2014, 15(9): 613-624.
[5]	汪艳杰, 龙鸿, 姚家玲. 亚硫酸氢钠测序法检测水稻FIE基因CpG岛甲基化状态[J].植物科学学报, 2011, 29(1): 134-139.
[6]	邓莹, 高乐旋, 朱珠, 杨继. 不同水陆环境中喜旱莲子草甲基化调控因子表达水平和差异表达基因启动子区甲基化状态分析[J].植物科学学报, 2014, 32(5): 475-486.
[7]	Kim JM, To TK, Nishioka T, Seki M. Chromatin regulation functions in plant abiotic stress responses[J].Plant Cell Environ, 2010, 33(4): 604-611.
[8]	Jaenisch R, Bird A. Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals[J].Nat Genet, 2003, 33(Suppl): 245-254.
[9]	Eichten SR, Schmitz RJ, Springer NM. Epigene-tics: beyond chromatin modifications and complex genetic regulation[J].Plant Physiol, 2014, 165(3): 933-947.
[10]	Bouché N, Fromm H. GABA in plants: just a metabolite?[J].Trends Plant Sci, 2004, 9(3): 110-115.
[11]	Yu GH, Liang JG, He ZK, Sun MX. Quantum dot-mediated detection of gamma-aminobutyric acid binding sites on the surface of living pollen protoplasts in tobacco[J].Chem Biol, 2006, 13(7): 723-731.
[12]	马兆武, 杨旭红, 余光辉. γ-氨基丁酸对烟草MAPK和ACS1基因表达的分叉调节[J].武汉植物学研究, 2008, 26(5): 520-523.
[13]	Liu CL, Zhao L, Yu GH. The dominant glutamic acid metabolic flux to produce γ-aminobutyric acid over proline in Nicotiana tabacum leaves under water stress relates to its significant role in antioxidant activity[J].J Integr Plant Biol, 2011, 53(8): 608-618.
[14]	张瑞琦, 赵丽, 覃永华, 徐鑫, 余光辉. γ-氨基丁酸促进拟南芥开花的机理研究[J].中国农学通报, 2012, 28(15): 142-147.
[15]	赵丽, 赵志龙, 龚汉雨, 覃永华, 余光辉. GABA对烟草花粉管Ca²⁺和K⁺的动态调节表明离子通道参与了花粉管生长的调控[J].中国细胞生物学报, 2013, 32(5): 668-675.
[16]	Yu GH, Zou J, Feng J, Peng XB, Wu JY, Wu YL, Palanivelu R, Sun MX. Exogenous γ-aminobutyric acid (GABA) affects pollen tube growth via modulating putative Ca²⁺-permeable membrane channels and is coupled to negative regulation on glutamate decarboxylase[J].J Exp Bot, 2014, 65(12): 3235-3248.
[17]	Law JA, Jacobsen SE. Establishing, maintaining and modifying DNA methylation patterns in plants and animals[J].Nat Rev Genet, 2010,11(3): 204-220.
[18]	Sahu PP, Pandey G, Sharma N, Puranik S, Muthamilarasan M, Prasad M. Epigenetic mechanisms of plant stress responses and adaptation[J].Plant Cell Rep, 2013, 32(8): 1151-1159.
[19]	Slotkin RK, Vaughn M, Borges F, Tanurdzic' M, Becker JD, Feijó JA, Martienssen RA. Epigenetic reprogramming and small RNA silencing of transposable elements in pollen[J].Cell, 2009, 136(3): 461-472.
[20]	Birnbaum K, Shasha DE, Wang JY, Jung JW, Lambert GM, Galbraith DW, Benfey PN. A gene expression map of the Arabidopsis root[J].Science, 2003, 302(5652): 1956-1960.
[21]	Yu GH, Peng XB, Cheng G, Liu XQ, Wang CT, Sun MX. Specifically proteomic identification of differentially expressed proteins linking pollen tube growth with γ-aminobutyric acid responses in Nicotiana tabacum pollen proteomics[C]//21st IUBMB & 12th FAOBMB International Congress of Biochemistry and Molecular Biology. Shanghai, 2009: 116.
[22]	Yu GH, Zhao L, Xiang WH, Qin YH, Xu X. Whole-genome scale analysis of gene expression profiling of Arabidopsis flowering promotion in responding to γ-aminobutyric acid signals[C]//2012 International Symposium on Epigenetic Regulation in Higher Plants (2012 ISERHP). Beijing: 2012.
[23]	Tang Y, Xiong J, Jiang HP, Zheng SJ, Feng YQ, Yuan BF. Determination of oxidation products of 5-methylcytosine in plants by chemical derivatization coupled with liquid chromatography/tandem mass spectrometry analysis[J].Anal Chem, 2014, 86(15): 7764-7772.
[24]	Brooks SC, Fischer RL, Huh JH, Eichman BF. 5-methylcytosine recognition by Arabidopsis thaliana DNA glycosylases DEMETER and DML3[J].Biochem, 2014, 53(15): 2525-2532.
[25]	Liu S, Dunwell TL, Pfeifer GP, Dunwell JM, Ullah I, Wang Y. Detection of oxidation products of 5-Methyl-29-Deoxycytidine in Arabidopsis DNA[J].PLoS One, 2013, 8(12): e84620.
[26]	Moricová P, Ondej V, Navrátilová B, Luhová L. Changes of DNA methylation and hydroxymethylation in plant protoplast cultures[J].Acta Biochim Pol, 2013, 60(1): 33-36.

[1]	Ai Ying, Liu Haikun, Yu Linhong, Hu Man, Dang Haishan. Study on non-structural carbohydrate allocation strategies of psammophytes induced by drought stress in southeastern Xizang[J]. Plant Science Journal, 2024, 42(5): 602-611. DOI: 10.11913/PSJ.2095-0837.23371
[2]	Xin Yaxuan, Tan Yunhong, Xin Peiyao, Li Haitao, Zhang Shuhong, Tang Junrong, Yu Wenbin. Taxonomical clarification of Dracaena cambodiana Pierre ex Gagnep., the source plant of Chinese “Resina Draconis”[J]. Plant Science Journal, 2024, 42(5): 572-581. DOI: 10.11913/PSJ.2095-0837.23340
[3]	Xin Jia, Sun Heng, Liu Juan, Yang Dong, Deng Xian-Bao, Yang Mei. Advances in the identification, function, and application of flavonoids in Nelumbo[J]. Plant Science Journal, 2023, 41(3): 400-410. DOI: 10.11913/PSJ.2095-0837.22194
[4]	Xiong Zhe-Ming, Yu Ming-Zhu, Yang Xing-Xing, Hu Chao-Yi, Xu Bo, Hu Zhi-Gang, Zhang Chong, Sen Lin, Peng Yuan. Study on full-length coding sequence of matK gene and its coding protein from medicinal Botrychium[J]. Plant Science Journal, 2020, 38(2): 233-240. DOI: 10.11913/PSJ.2095-0837.2020.20233
[5]	Zhang Tian-Yuan, Wang Bai-Shi, Song Chi, Zhang Yan-Jun, Xu Xiao-Yu, Yang Xue-Ying, Zhang Jin, Zhang Ying, Liu Kai-Hui, Peng Xiao-Tian, Pei Li. Identification of Vitis species based on nuclear gene molecular polymorphism[J]. Plant Science Journal, 2018, 36(4): 569-574. DOI: 10.11913/PSJ.2095-0837.2018.40569
[6]	WANG Qiu-Li, YANG Yun-Qiang, YANG Yong-Ping. Molecular Cloning, Sequence Analyses, and Functional Identification the of WRKY53 Gene in Stipa purpurea[J]. Plant Science Journal, 2016, 34(6): 879-887. DOI: 10.11913/PSJ.2095-0837.2016.60879
[7]	QIAO Hai-Yun, LI Fei, ZHANG Shu-Jiang, ZHANG Shi-Fan, ZHANG Hui, SUN Ri-Fei. Production and Identification of Interspecific Hybrids between Chinese Cabbage and Purple Cabbage[J]. Plant Science Journal, 2012, 30(4): 407-414. DOI: 10.3724/SP.J.1142.2012.40407
[8]	ZHONG Cai-Hong, ZHANG Peng, JIANG Zheng-Wang, WANG Sheng-Mei, HAN Fei, XU Li-Yun, HUANG Hong-Wen. Dynamic Changes of Carbohydrate and Vitamin C in Fruits of Actinidia chinensis and A.eriantha during Growing Season[J]. Plant Science Journal, 2011, 29(3): 370-376.
[9]	LUO Yu, WU Yao-Sheng, ZHOU Juan, LI Ke-Zhi, XU Peng. RAPD Analysis with Random Primer Combinations and SCAR Identification of Gynostemma pentaphyllum[J]. Plant Science Journal, 2011, 29(2): 243-247.
[10]	QIU Qing-Chuan, YANG Dai-Chang. Molecular Pharming in Plants:An Overview and Update[J]. Plant Science Journal, 2009, 27(1): 83-89.

Cited By

Get Citation

PDF

XML

Article views (1114) PDF downloads (1316)

Turn off MathJax

Article Contents

Abstract

References

Dynamic Changes of Methylcytosine in Polarized Cell Growth and Their Response to GABA Signals

Abstract

References

Related Articles

Catalog

Dynamic Changes of Methylcytosine in Polarized Cell Growth and Their Response to GABA Signals

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content