Study on the Anatomical Structures of <i>Rhododendron protistum</i> var. <i>giganteum</i> with an Extremely Small Population

SHEN Shi-Kang; ZHANG Xin-Jun; WU Fu-Qin; YANG Guan-Song; WANG Yue-Hua; SUN Wei-Bang; LIN Ru-Tao

doi:10.11913/PSJ.2095-0837.2016.10001

Plant Science Journal > 2016 > 34(1): 1-8. > DOI: 10.11913/PSJ.2095-0837.2016.10001 CSTR: 32231.14.PSJ.2095-0837.2016.10001

SHEN Shi-Kang, ZHANG Xin-Jun, WU Fu-Qin, YANG Guan-Song, WANG Yue-Hua, SUN Wei-Bang, LIN Ru-Tao. Study on the Anatomical Structures of Rhododendron protistum var. giganteum with an Extremely Small Population[J]. Plant Science Journal, 2016, 34(1): 1-8. DOI: 10.11913/PSJ.2095-0837.2016.10001

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Study on the Anatomical Structures of Rhododendron protistum var. giganteum with an Extremely Small Population

1. School of Life Sciences, Yunnan University, Kunming 650091, China;
2. Kunming Botanical Garden, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
3. Administration of Gaoligongshan Mountains National Nature Reserve, Baoshan, Yunnan 678000, China

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This work was supported by grants from the National Natural Science Foundation of China (31360155) and National Natural Science Foundation of China-Yunnan (U1302262).

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Received Date: July 17, 2015
Available Online: October 31, 2022
Published Date: February 27, 2016

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Abstract

Rhododendron protistum var.giganteum is an evergreen tree that belongs to subgenus Hymenanthes and subsection Grandia from the family Ericaceae.It is a highly endangered species in a small area in the southern part of Yunnan Province in China.This plant has been listed in the Conservation Program for Wild Plants with Extremely Small Populations in China (2012-2015 Operational Plan) due to its limited distribution and small populations.In the present study, the anatomical characteristics of the leaves, stems and roots of R.protistum var.giganteum were studied by paraffin sectioning and leaf epidermal isolation.Furthermore, seed surface, stoma and indumentum characteristics were also observed under scanning electron microscope (SEM).The paraffin sectioning results indicated that the secondary structure of the root was mainly composed of periderm and vascular cylinder.The anatomical structure of the stem consisted of the epidermis, cortex, phloem, xylem and pith.The leaf was bifacial with a cuticle on the upper epidermis.The epidermal hairs and stomata were only distributed on the lower epidermis.The stomata were anomocytic type.Palisade tissue was arranged closely and was well-developed, while the spongy tissue was arranged loosely.There were well-developed vascular bundles and bundle sheath extensions distributed in the structure of the leaf.Through SEM, it was found that the foliar trichome of R.protistum var. giganteum was composed of two layers of epidermal hairs, including a layer of cavernous villi and a layer of short lamelliform villi.Basal and apical wings were equally developed on the seeds.Seeds were flattened and exotesta longitudinal sculptures, which were conformed on the dorsal and ventral sides of the seed.The results obtained in the present study will not only help to further understand the biological characteristics of this species, but also provide a scientific basis to elucidate its systematic classification and habitat adaptation.
- Rhododendron,
- Classification,
- Anatomical structure,
- Adaptation,
- Epidermal hair,
- Endangered plant

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References

[1]	Noshiro S, Suzuki M.Ontogenetic wood anatomy of tree and subtree species of Nepalese Rhododendron (Erica-ceae) and characterization of shrub species[J].Am J Bot, 2001, 88(4): 560-569.
[2]	Poli LP, Temponi LG, Coan AI.Anatomical aspects of the gynoecium of species of Anthurium sect.Urospadix Engl.(Araceae,Alismatales) [J].Flora, 2012, 207(8): 615-621.
[3]	白潇,李毅,苏世平,赵小仙.不同居群唐古特白刺叶片解剖特征对生境的响应研究[J]. 西北植物学报,2013,33(10): 1986-1993. Bai X, Li Y, Su SP, Zhao XX.Response of leaf anatomical characteristics of Nitraria tangutorum Bobr.from different populations to habitats[J].Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(10): 1986-1993.
[4]	闵天禄,方瑞征.杜鹃属的系统发育与进化[J].云南植物研究, 1990, 12(4): 353-365. Min TL, Fang RZ.The phylogeny and evolution of genus Rhododendron[J].Acta Botanica Yunnanica, 1990, 12(4): 353-365.
[5]	王玉国,李光照,张文驹,尤佳,陈家宽.中国杜鹃花属的叶表皮特征及其系统学意义[J]. 植物分类学报, 2007, 45(1): 1-20. Wang YG, Li GZ, Zhang WJ, You J, Chen JK.Leaf epidermal features of Rhododendron (Ericaceae) from China and their systematic significance[J].Acta Phytotaxonomica Sinica, 2007, 45(1): 1-20.
[6]	Chamberlaind DF, Hyam R, Argent G.The Genus Rhododendron: Its Classification and Synonymy[M].Edinburgh: Royal Botanic Garden Edinburgh, 1996.
[7]	邹璞,廖景平,张奠湘.羊蹄甲属植物种子表面微形态观察[J].广西植物, 2008, 28(1): 24-32. Zou P, Liao JP, Zhang DX.Spermodermal micro-morphology of Bauhinia (Leguminosae: Caesalpinioideae) [J].Guihaia, 2008, 28(1): 24-32.
[8]	耿玉英.中国杜鹃花属植物[M].上海: 上海科学技术出版社, 2014. Geng YY.The Genus of Rhododendron in China[M].Shanghai: Shanghai Science and Technology Press, 2014.
[9]	苗燕,王宁果,李伟,彭勇,王丽,蔡霞.陕西分布10种大戟属植物茎叶形态结构比较[J].西北植物学报,2014, 34(11): 2243-2254. Miao Y, Wang NG, Li W, Peng Y, Wang L, Cai X.Analysis on morphology and structure of the leaf and stem of 10 Euphorbia species in Shaanxi Province[J].Acta Botanica Boreali-Occidentalia Sinica, 2014, 34(11): 2243-2254.
[10]	Ueda R, Minamiya Y, Hirata A, Hayakawa H, Muramatsu Y, Saito M, Fukuda T.Morphological and anatomical analyses of rheophytic Rhododendron ripense Makino (Ericaceae)[J].Plant Spec Biol, 2012, 27(3): 233-240.
[11]	熊子仙,杜青.国产杜鹃花叶解剖与分类群[J].广西植物, 2000, 20(4): 335-338. Xiong ZX, Du Q.Taxon and anatomy of leaves in Rhododendron from China[J].Guihaia, 2000, 20(4): 335-338.
[12]	周兰英,王永清,张丽.26 种杜鹃属植物花粉形态及分类学研究[J]. 林业科学, 2008,44(2): 55-63. Zhou LY, Wang YQ, Zhang L.Pollen morphology and its taxonomy of twenty-six species in Rhododendron[J].Scientia Silvae Sinicae, 2008, 44(2): 55-63.
[13]	容丽,陈训,汪小春.百里杜鹃杜鹃属13种植物叶片解剖结构的生态适应性[J].安徽农业科学, 2009, 37(3): 1084-1088. Rong L, Chen X, Wang XC.Leaf anatomical characters and its ecological adaptation of 13 species of Rhododendron in Baili Azalea area[J].Journal of Anhui Agricultural Science, 2009,37(3), 1084-1088.
[14]	Wu FQ, Shen SK, Zhang XJ, Wang YH, Sun WB.Genetic diversity and population structure of an extremely enda-ngered species-the world's largest Rhododendron[J].AoB Plants, 2015, 7(82): 1-8.
[15]	Cox PA.The Encyclopedia of Rhododendron Species[M].Singapore: Toppam Printing Co Ltd, 1997.
[16]	Davidian HH.The Rhododednron Species: Vol 4.Aza-leas[M].Portland: Timber Press, 1982.
[17]	Fang MY, Fang RZ, He MY.Rhododendron (Ericaceae) [M]/Wang XW, Mao ZJ, Choi K, Park K.Significance of the leaf epidermis fingerprint for taxonomy of genus Rhododendron[J].J Forest Res, 2006, 17(3): 171-176.
[20]	肖寒,白洁.常绿杜鹃亚属云锦杜鹃亚组26种植物叶片微形态特征及其分类学意义[J].四川大学学报: 自然科学版,2013, 50(2): 377-384. Xiao H, Bai J.Leaf micromorphological characteristics and taxonomic significance of twenty-six species of subsect.Fortunes.subgen.Hymenanthe (Ericaceae)[J].Journal of Sichuan University:Natural Science Edition, 2013, 50(2): 377-384.
[21]	唐光大,吴海求,庄雪影.杜鹃花属 5 种植物叶表皮结构解剖及分类意义初探[J].湖北民族学院学报: 自然科学版, 2010, 28(4): 371-374. Tang GD, Wu HQ, Zhuang XY.Leaf epidermal features and their taxonomic significance of five species of Rhododendron in China[J].Journal of Hubei University for Nationalities:Natural Science Edition, 2010, 28(4): 371-374.
[22]	陈翔,黄承玲,张玉武,李苇洁,龙成昌.贵州30种杜鹃属植物叶背表皮显微形态特征[J].广西植物, 2015,35(1): 20-30. Chen X, Huang CL, Zhang YW, Li WJ, Long CC.Micromorphological characters of leaf abaxial epidermis for 30 species of Rhododendron (Ericaceae) from Guizhou Province[J].Guihaia, 2015, 35(1): 20-30.
[23]	王桂芹,刘艳然.都支杜鹃茎、叶解剖特征与环境的适应性[J].植物研究, 2012, 32(5): 532-536. Wang GQ, Liu YR.Anatomy features and environment flexibility of Rhododendron shanii Fang[J].Bulletin of Botanical Research, 2012, 32(5): 532-536.
[24]	丁炳扬,吴欢笑,张慧明,方云亿.浙江杜鹃花属植物种子形态及其分类学意义[J].西北植物学报, 1995, 15(6): 36-42. Ding BY, Wu HX, Zhang HM, Fang YY.Seed morphology of Rhododendron 1.(Ericaceae) from Zhejian and its taxonomic significance[J].Acta Botanica Boreali-Occidentalia Sinica, 1995, 15(6): 36-42.
[25]	陈翔,黄家勇,张玉武,陈训.贵州13种杜鹃属植物种子显微形态研究[J].种子, 2012, 31(10): 66-70. Chen X, Huang JY, Zhang YW, Chen X.Study on the micromorphological characters of seed for 13 species of Rhododendron (Ericaceae) from Guizhou Province[J].Seed, 2012, 31(10): 66-70.
[26]	王玉国,李光照,张文驹,尤佳,陈家宽.果皮和种皮微形态特征在杜鹃花属系统学研究中的应用[J].植物分类学报, 2007, 45(1): 21-38. Wang YG, Li GZ, Zhang WJ, You J, Chen JK. A syste-matic study of the genus Rhododendron (Ericaceae) using micromorphological characters of fruit surface and seed coat[J].Acta Phytotaxonomica Sinica, 2007, 45(1): 21-38.
[27]	Kingdon-Ward F.Rhododendron seed, with special refe-rence to their classification[J]. J Bot, 1935, 73: 241-247.
[28]	刘旭颖,沈向群,张艳红.耐寒杜鹃叶片结构研究[J].湖北农业科学, 2010, 49(8):1903-1905. Liu XY, Shen XQ, Zhang YH.Study on the leaf structure of hardy Rhododendron[J].Hubei Agricultural Sciences, 2010, 49(8):1903-1905.
[29]	中国植物志编辑委员会.中国植物志: 第57卷, 第2分册[M].北京: 科学出版社, 1994. China Flora Editorial Board.Flora of China: Vol.57 (2) [M].Beijing: Science Press, 1994.
[30]	中国科学院昆明植物研究所.云南植物志: 第4卷[M].北京: 科学出版社, 1986.

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Study on the Anatomical Structures of Rhododendron protistum var. giganteum with an Extremely Small Population

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