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DONG Chao, YANG Yun-Qiang, SUN Xu-Dong, LI Xiong, YANG Shi-Hai, HUANG Rong, YANG Yong-Ping. Molecular Cloning and Expression of ScTIP1;1 in Stipa capillacea under Abiotic Stress[J]. Plant Science Journal, 2016, 34(1): 99-108. DOI: 10.11913/PSJ.2095-0837.2016.10099
Citation: DONG Chao, YANG Yun-Qiang, SUN Xu-Dong, LI Xiong, YANG Shi-Hai, HUANG Rong, YANG Yong-Ping. Molecular Cloning and Expression of ScTIP1;1 in Stipa capillacea under Abiotic Stress[J]. Plant Science Journal, 2016, 34(1): 99-108. DOI: 10.11913/PSJ.2095-0837.2016.10099
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Molecular Cloning and Expression of ScTIP1;1 in Stipa capillacea under Abiotic Stress

  • 1. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, China;

  • 2. Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201 China;

  • 3. University of Chinese Academy of Sciences, Beijing, 100049 China;

  • 4. Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 10010 China

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This work was supported by grants from the National Natural Science Foundation of China (41271058), National Basic Research Program of China (2010CB951704), and Open Project of Key Laboratory of Land Surface Process and Climate in Cold and Arid Regions, Chinese Academy of Sciences (Y5203511Y1).

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  • Received Date: September 01, 2015
  • Available Online: October 31, 2022
  • Published Date: February 27, 2016
    Graphical Abstract
    • Abstract
  • Based on EST sequence and RACE experiments, the full-length coding sequence of a tonoplast intrinsic protein (TIP) gene termed ScTIP1;1 was cloned from Stipa capillacea. Sequence analysis showed that the opening reading frame (ORF) of ScTIP1;1 was 753 bp, encoding a 250 amino acid peptide with a protein molecular weight of 25.8 kD and isoelectric point of 6.16. Phylogenetic analysis and sequence alignment results indicated that ScTIP1;1 had a close relationship with AtTIP1;1 of Arabidopsis thaliana. Subcellular localization analysis indicated that ScTIP1;1 was localized in the tonoplast membrane. The expression level of ScTIP1;1 was induced by salt, drought and especially low temperature in the leaves of S. capillacea. These results provide new insight into understanding the ecological adaptations of S.capillacea.
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    • References (29)
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