Effect on Root Length of the Overexpression of <em>Arabidopsis VHA-c</em>4 and <em>VHA-c</em>5 Genes

SU Jie; GUO Rong-Qi; LI Guo-Jing; WANG Rui-Gang

doi:10.11913/PSJ.2095-0837.2016.50765

Plant Science Journal > 2016 > 34(5): 765-774. > DOI: 10.11913/PSJ.2095-0837.2016.50765 CSTR: 32231.14.PSJ.2095-0837.2016.50765

SU Jie, GUO Rong-Qi, LI Guo-Jing, WANG Rui-Gang. Effect on Root Length of the Overexpression of Arabidopsis VHA-c4 and VHA-c5 Genes[J]. Plant Science Journal, 2016, 34(5): 765-774. DOI: 10.11913/PSJ.2095-0837.2016.50765

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Effect on Root Length of the Overexpression of Arabidopsis VHA-c4 and VHA-c5 Genes

1.
Vocational and Technical College of Inner Mongolia Agricultural University, Tuyouqi, Inner Mongolia 014109, China
2. College of Life Sciences Inner Mongolia Agricultural University, Hohhot 010018, China
3. Agricultural Sciences of Hulunbeier, Yakeshi, Inner Mongolia 162650, China

Funds: This work was supported by grants from the National Natural Science Foundation of China (30460018) and the Technology Innovation Team of Inner Mongolia Autonomous Region (201503004).

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Received Date: March 23, 2016
Revised Date: May 09, 2016
Published Date: October 27, 2016

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Abstract

Abstract

To study the role of vacuole H⁺-ATPase subunit c genes on plant growth and development, over-expression vectors of VHA-c4 and VHA-c5 from Arabidopsis thaliana were constructed and introduced into wild-type A. thaliana, and 9 and 7 homozygous T2 generations of VHA-c4 and VHA-c5 overexpressed transgenic lines were obtained, respectively. Semi-quantitative RT-PCR methods were used for identification of the positive VHA-c4 and VHA-c5 transgenic homozygotes, and their corresponding transcript levels were increased in every transgenic line. When cultivated under different light conditions, the VHA-c4 transgenic plants exhibited a reduction in primary root length compared with that of the wild-type when cultivated in the dark, whereas the VHA-c5 transgenic plants showed reduced primary root length under normal light conditions. These results indicated that VHA-c4 and VHA-c5 affected the growth of roots during light/dark cycles. Following ABA and sugar (glucose and sucrose) treatment, no significant differences were found between the transgenic homozygote phenotype and wild type, indicating that VHA-c4 and VHA-c5 overexpression did not affect the response of Arabidopsis to ABA or sugar.
- Arabidopsis thaliana,
- VHA-c4,
- VHA-c5,
- Dark,
- Normal light,
- ABA,
- Sugar

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References (35)

References

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