Molecular Cloning, Sequence Analyses, and Functional Identification the of <i>WRKY53</i> Gene in <i>Stipa purpurea</i>

WANG Qiu-Li; YANG Yun-Qiang; YANG Yong-Ping

doi:10.11913/PSJ.2095-0837.2016.60879

Plant Science Journal > 2016 > 34(6): 879-887. > DOI: 10.11913/PSJ.2095-0837.2016.60879 CSTR: 32231.14.PSJ.2095-0837.2016.60879

WANG Qiu-Li, YANG Yun-Qiang, YANG Yong-Ping. Molecular Cloning, Sequence Analyses, and Functional Identification the of WRKY53 Gene in Stipa purpurea[J]. Plant Science Journal, 2016, 34(6): 879-887. DOI: 10.11913/PSJ.2095-0837.2016.60879

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Molecular Cloning, Sequence Analyses, and Functional Identification the of WRKY53 Gene in Stipa purpurea

1. School of Life Sciences, Yunnan University, Kunming 650201, China;
2. Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
3. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (41271058), National Basic Research Program of China (2010CB951704) and Open Project of Key Laboratory of Land Surface Process and Climate in Cold and Arid Regions in the Chinese Academy of Sciences (Y5203511Y1).

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Received Date: April 25, 2015
Revised Date: May 30, 2016
Available Online: October 31, 2022
Published Date: December 27, 2016

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Abstract

Abstract

The gene family of the WRKY transcription factors in plants play an important role in controlling leaf senescence. The WRKY53 gene is closely related to leaf senescence. Based on the EST sequence, the full-length CDS of SpWRKY53 was cloned from Stipa purpurea. Sequence analysis showed that the opening reading frame of SpWRKY53 was 1443 bp, which encoded a 480-amino acid peptide. Its protein molecular weight and isoelectric point were 50.9 kD and 7.64, respectively. Sequence alignment and phylogenetic analysis results indicated that the SpWRKY53 gene with two WRKY sequences displayed similar structures with those reported for the WRKY53 gene of wheat. SpWRKY53 was localized in the cell nucleus by subcellular analysis. SpWRKY53 was upregulated and could promote leaf senescence under abiotic stress. This study will lay a foundation for further study of the WRKY53gene in the molecular breeding of forage grass.
- Stipa purpurea,
- SpWRKY53,
- Molecular cloning,
- Gene expression,
- Leaf senescence

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References (29)

References

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Molecular Cloning, Sequence Analyses, and Functional Identification the of WRKY53 Gene in Stipa purpurea

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