Overexpression of the Cotton <i>CBF2</i> Gene Enhances Salt and Drought Tolerance in <i>Arabidopsis thaliana</i>

CHEN Yun; ZHEN Yong; LIU Xia; REN Yu; MA Liu-Feng

doi:10.11913/PSJ.2095-0837.2016.60888

Plant Science Journal > 2016 > 34(6): 888-900. > DOI: 10.11913/PSJ.2095-0837.2016.60888 CSTR: 32231.14.PSJ.2095-0837.2016.60888

CHEN Yun, ZHEN Yong, LIU Xia, REN Yu, MA Liu-Feng. Overexpression of the Cotton CBF2 Gene Enhances Salt and Drought Tolerance in Arabidopsis thaliana[J]. Plant Science Journal, 2016, 34(6): 888-900. DOI: 10.11913/PSJ.2095-0837.2016.60888

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Overexpression of the Cotton CBF2 Gene Enhances Salt and Drought Tolerance in Arabidopsis thaliana

1. College of Life and Geography Science of Kashgar University, Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges & Universities under the Department of Education of Xinjiang Uygur Autonomous Region, Kashgar, Xinjiang 844000, China;
2. College of Life Sciences of Xinjiang Normal University, Urumqi 830013, China;
3. Central China Normal University, Wuhan 430079, China

Funds:

This work was supported by grants from the Scientific Research Program of the Higher Education Institution of Xin Jiang (Grant No. XJEDU2014I037) and the Starting Fund for High-Scientific Study of Genius of Kashgar University (GCC16ZK-002).

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Received Date: May 04, 2016
Revised Date: May 29, 2016
Available Online: October 31, 2022
Published Date: December 27, 2016

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Abstract

Abstract

CBF/DREB proteins are transcription factors in plants, which play important biological functions in cold, drought, and salt stress resistance. In this study, a CBF/DREB gene, GhCBF2, was cloned from Gossypium hirsutum L., coding a protein consisting of 216 amino acids. Sequence analysis indicated that GhCBF2 contained a typical AP2 conservative domain structure, much like the CBF proteins in other plant species. The transcript of the GhCBF2 gene in cotton seedlings was upregulated following exposure to drought and salt stress. Subcellular localization of proteins showed that the GhCBF2-GFP fusion protein was localized to the nucleus. To study the function of the GhCBF2 gene in drought and salt stress resistance, it was inserted into pMD to construct a GhCBF2-overexpression vector under the control of CaMV 35S promoter. The construct was introduced into Arabidopsis thaliana by the floral dip method. Analysis of resistance to drought and salt stress showed that the survival rate of transgenic A. thaliana plants was improved remarkably compared to that of the wildtype (WT) plants. The proline and soluble sugar contents in the transgenic plants were also higher than those in the WT plants. These results show that GhCBF2 can enhance transgenic plant tolerance to drought and salt stress. We selected several stress related genes, including COR15A, RD29A, and ERD6, and examined their expression by quantitative RT-PCR in both transgenic and WT plants. Remarkably, the expressions of these marker genes in GhCBF2-overexpressed transgenic plants were substantially higher than those in WT plants, indicating that the GhCBF2 gene is involved in the regulation of drought and salt related genes.
- Cotton,
- GhCBF2 gene,
- Transgenic Arabidopsis thaliana,
- Drought stress,
- Salt stress

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References (36)

References

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Overexpression of the Cotton CBF2 Gene Enhances Salt and Drought Tolerance in Arabidopsis thaliana

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