Analysis on the genetic diversity of endangered <i>Isoetes sinensis</i> Palmer from China based on the second intron of <i>LEAFY</i>

Huang Yu-Qian; Li Xiang; Zhou Ya-Dong; Li Xiao-Yan; Liu Xing

doi:10.11913/PSJ.2095-0837.2017.10073

Plant Science Journal > 2017 > 35(1): 73-78. > DOI: 10.11913/PSJ.2095-0837.2017.10073 CSTR: 32231.14.PSJ.2095-0837.2017.10073

Huang Yu-Qian, Li Xiang, Zhou Ya-Dong, Li Xiao-Yan, Liu Xing. Analysis on the genetic diversity of endangered Isoetes sinensis Palmer from China based on the second intron of LEAFY[J]. Plant Science Journal, 2017, 35(1): 73-78. DOI: 10.11913/PSJ.2095-0837.2017.10073

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Analysis on the genetic diversity of endangered Isoetes sinensis Palmer from China based on the second intron of LEAFY

1. Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China;
2. Experimental Teaching Center, Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China;
3. Faculty of Science, Tibet University, Lhasa 850012, China

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This work was supported by a grant from the National Natural Science Foundation of China(31170203).

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Received Date: May 10, 2016
Available Online: October 31, 2022
Published Date: February 27, 2017

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Abstract

Abstract

We used the second intron of the LEAFY gene to analyze the genetic diversity of Isoetes sinensis Palmer from China. In total, 105 samples were collected from seven populations. From these I. sinensis samples, 78 haplotypes were discovered, with a haplotype diversity of 0.989 and nucleotide diversity of 0.021. Results showed that genetic differentiation coefficient G_ST=0.283, N_ST=0.298, G_ST>N_ST, N_m=0.59; Mantel test, Rxy=-0.286, P (rxy-rand≥rxy-data)=0.370, and 72% of the total variation existed within populations. UPGMA cluster showed that the two high altitude populations had distant genetic relationships with the other five populations. The neutrality test, Tajima's D, Fu & Li's D^* and F^* tests were negative, and the mismatch distribution test based on a stable model was multimodal. According to the geographic location of I. sinensis, its genetic structure was related to the drainage, altitude, and process of hybrid polyploidization.
- Isoetes sinensis Palmer,
- LEAFY gene,
- Genetic diversity

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References (30)

References

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Analysis on the genetic diversity of endangered Isoetes sinensis Palmer from China based on the second intron of LEAFY

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