Isolation and functional characterization of the <i>ASR</i> gene from <i>Ipomoea pes-caprae</i>

Zhang Hui; Zheng Jie-Xuan; Jian Shu-Guang; Xia Kuai-Fei; Zhang Mei

doi:10.11913/PSJ.2095-0837.2018.30402

Plant Science Journal > 2018 > 36(3): 402-410. > DOI: 10.11913/PSJ.2095-0837.2018.30402 CSTR: 32231.14.PSJ.2095-0837.2018.30402

Zhang Hui, Zheng Jie-Xuan, Jian Shu-Guang, Xia Kuai-Fei, Zhang Mei. Isolation and functional characterization of the ASR gene from Ipomoea pes-caprae[J]. Plant Science Journal, 2018, 36(3): 402-410. DOI: 10.11913/PSJ.2095-0837.2018.30402

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Isolation and functional characterization of the ASR gene from Ipomoea pes-caprae

1. Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

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This work was supported by grants from the National Key Research and Development Program of China (2016YFC1403002), ‘Strategic Priority Research Program’ of the Chinese Academy of Sciences (XDA13020500), and "Twelfth Five-Year" National S & T Projects for Rural Areas (2015BAL04B04).

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Received Date: October 23, 2017
Available Online: October 31, 2022
Published Date: June 27, 2018

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Abstract

Abstract

We focused on the full-length cDNA encoding ASR protein isolated from a Ipomoea pes-caprae (Linn.) Sweet. cDNA library. Results showed the coding region of IpASR cDNA was 648 bp, encoding a 215 amino acid protein with a molecular weight of 24.57 kD and isoelectric point of 5.42. By ectopic expression of IpASR in yeast, we found that IpASR improved the salt and H₂O₂ tolerance of transgenic yeast strains. Using adult plants and seedlings of I. pes-caprae with or without abiotic stress and ABA treatment, real-time RT-PCR analysis showed that IpASR was widely expressed in different adult organs in I. pes-caprae. The IpASR transcript was induced under abiotic stress and ABA treatment. The subcellular localization assay combining bioinformatics analysis showed that IpASR was a nucleoprotein. These results suggest that IpASR might play an important role in the regulation of I. pes-caprae growth and development, and therefore respond to environmental abiotic stress and the ABA signal pathway.
- Ipomoea pes-caprae,
- ASR,
- Abiotic stress,
- Subcellular localization

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References

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Isolation and functional characterization of the ASR gene from Ipomoea pes-caprae

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