Genome-wide identification and expression analysis of TPS genes in moso bamboo (Phyllostachys edulis)

To explore the characteristics and evolutionary relationships of the TPS family in Phyllostachys edulis, we identified the TPS genes in P.edulis and analyzed their physicochemical properties, gene structure, evolutionary relationship, protein secondary and tertiary structure, promoter elements, and expression patterns based on bioinformatics methods. A total of 14 TPS genes were identified in P. edulis, which ranged in size from 693 to 2439 bp. The encoding protein isoelectric points ranged from 5.08 to 8.17. Phylogenetic analysis suggested that the TPS family members from P. edulis could be divided into four subfamilies (a, b, e/f, g), with considerably different gene structures. The α-helix and random coil components in these proteins were dominant elements, and the predicted tertiary structure of the proteins in the PeTPS gene family were similar. We identified 50 cis-acting regulatory elements through promotor analyses, which were classified into six categories according to their function. The heatmap of gene expression based on the RNA-seq data revealed that the PeTPS genes were expressed differently among seven different tissues, including leaves, flowers, and shoots, and thus exhibited tissue-specific expression patterns. PeTPS9 was only expressed in the early panicle and PeTPS8 was only expressed in the leaves. This research provides a theoretical foundation for deeper analysis of the function of the TPS genes in P.edulis.