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Lü Ding-Hao, Liu An-Li, Xu Wen-Fen, He Shun-Zhi. Study on the genus Aspidistra based on DNA barcoding[J]. Plant Science Journal, 2018, 36(6): 784-789. DOI: 10.11913/PSJ.2095-0837.2018.60784
Citation: Lü Ding-Hao, Liu An-Li, Xu Wen-Fen, He Shun-Zhi. Study on the genus Aspidistra based on DNA barcoding[J]. Plant Science Journal, 2018, 36(6): 784-789. DOI: 10.11913/PSJ.2095-0837.2018.60784
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Study on the genus Aspidistra based on DNA barcoding

  • 1. The Fourth People's Hospital of Zunyi, Zunyi 563004, China;

  • 2. The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;

  • 3. Guiyang College of Traditional Chinese Medicine, Guiyang 550001, China

Funds: 

This work was supported by grants from the National Natural Science Foundation of China (8160640), Science and Technology Planning Project of Guizhou Province of China (LH[2016]7132), and First-class Discipline Construction Project of Guizhou Provincial Education Department (GNYL[2017]008).

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  • Received Date: June 07, 2018
  • Available Online: October 31, 2022
  • Published Date: December 27, 2018
    Graphical Abstract
    • Abstract
  • In this study, plant DNA barcode (matK, psbA-trnH, psbK-psbI, and rbcL) candidate sequences were used for the PCR amplification and sequencing of 104 samples from 19 Aspidistra species. The amplification success rate, sequencing success rate, and intra- and inter-species genetic variation of each barcode sequence were investigated, with BLAST searching adopted to evaluate the identification efficiency of each sequence. Results showed that the identification success rate of psbK-psbI was 88.7%, which was the highest for a single sequence. The identification efficiency of multiple combinational sequences was also compared and found to be significantly higher than that of the single sequences, with the success rate of matK+(psbK-psbI) reaching 100%. In addition, the neighbor-joining tree constructed based on the matK+(psbK-psbI) combinational sequence showed that samples of the same species had better aggregation and were mostly monophyletic. Therefore, matK+(psbK-psbI) appears to be the best barcode sequence for interspecific identification of Aspidistra.
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    • References (23)
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