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Li Wen-Yue, Lin Chun-Jing, Ding Xiao-Yang, Han Ya-Li, Peng Bao, Zhao Li-Mei, Zhang Chun-Bao. An improved method to extract Glycine max mitochondrial DNA[J]. Plant Science Journal, 2018, 36(6): 888-892. DOI: 10.11913/PSJ.2095-0837.2018.60888
Citation: Li Wen-Yue, Lin Chun-Jing, Ding Xiao-Yang, Han Ya-Li, Peng Bao, Zhao Li-Mei, Zhang Chun-Bao. An improved method to extract Glycine max mitochondrial DNA[J]. Plant Science Journal, 2018, 36(6): 888-892. DOI: 10.11913/PSJ.2095-0837.2018.60888

An improved method to extract Glycine max mitochondrial DNA

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This work was supported by grants from the Major Project for Science and Technology Development of Jilin Province(20170201001NY), National Natural Science Foundation of China(31601322), and Agricultural Science Technology Innovation Project of Jilin Province(CXGC2017JQ004, CXGC2017TD002, C7208000409).

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  • Received Date: June 13, 2018
  • Available Online: October 31, 2022
  • Published Date: December 27, 2018
  • In this study, yellowing Glycine max(L.) Merrill seedlings were used to extract mitochondrial DNA(mtDNA) by optimizing centrifugal force and time during differential centrifugation. Furthermore, establishing different sucrose density gradients and lysate concentrations during purification, combined with high salt removal protein, improved the extraction method of soybean mtDNA. Results showed that the concentration and purity of mtDNA extracted by this method were high, with no chloroplast or nuclear genomic DNA contamination, the mtDNA could be used for subsequent research on soybean mitochondrial genome.
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