Estimation of genome sizes of plants from <i>Ottelia</i> Pers. based on flow cytometry

Yan Dong-Ying; Li Yan; Sun Wen-Li; Chen Jin-Ming; Li Zhi-Zhong

doi:10.11913/PSJ.2095-0837.2020.20173

Plant Science Journal > 2020 > 38(2): 173-180. > DOI: 10.11913/PSJ.2095-0837.2020.20173 CSTR: 32231.14.PSJ.2095-0837.2020.20173

Yan Dong-Ying, Li Yan, Sun Wen-Li, Chen Jin-Ming, Li Zhi-Zhong. Estimation of genome sizes of plants from Ottelia Pers. based on flow cytometry[J]. Plant Science Journal, 2020, 38(2): 173-180. DOI: 10.11913/PSJ.2095-0837.2020.20173

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Estimation of genome sizes of plants from Ottelia Pers. based on flow cytometry

1. Research Center for Ecology and Environment of Qinghai-Tibetan Plateau, Tibet University, Lhasa 850000, China;
2. College of Science, Tibet University, Lhasa 850000, China;
3. Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
4. University of Chinese Academy of Sciences, Beijing 100049, China;
5. Huazhong Agricultural University, Wuhan 430070, China

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This work was supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31000000) and Student Innovation Training Program of the Chinese Academy of Sciences.

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Received Date: September 26, 2019
Revised Date: November 10, 2019
Available Online: October 31, 2022
Published Date: April 27, 2020

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Abstract

Abstract

The genome sizes of six taxa and three doubtful species of Ottelia were evaluated using flow cytometry (FCM) with the modified lysate P1. Nelumbo nucifera Gaertn. Fruct. et Semin was used as a reference species. The smallest C-value (6.759 pg) was detected in Ottelia cordata (Wall.) Dandy, whereas the largest C-value (12.929 pg) was found in Ottelia guanyangensis Z. Z. Li, S. Wu & Q. F. Wang. An ambiguous correlation was detected between the phylogenetic relationships within Ottelia and genome size. Furthermore, evaluation of phylogenetic status based on molecular systematics demonstrated that the three doubtful species were diploid and maintained a close relationship with Ottelia acuminata var. songmingensis Z. T. Jiang, H. Li & Z. L. Dao and O. guanyangensis, but a distant relationship with Ottelia cordata. Our study provides basic genomic resources for future phylogenetic and genomic research on the genus Ottelia.
- Ottelia,
- Genome size,
- Phylogeny,
- Flow cytometry

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References (34)

References

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