Study on full-length coding sequence of <i>matK</i> gene and its coding protein from medicinal <i>Botrychium</i>

Xiong Zhe-Ming; Yu Ming-Zhu; Yang Xing-Xing; Hu Chao-Yi; Xu Bo; Hu Zhi-Gang; Zhang Chong; Sen Lin; Peng Yuan

doi:10.11913/PSJ.2095-0837.2020.20233

Plant Science Journal > 2020 > 38(2): 233-240. > DOI: 10.11913/PSJ.2095-0837.2020.20233 CSTR: 32231.14.PSJ.2095-0837.2020.20233

Xiong Zhe-Ming, Yu Ming-Zhu, Yang Xing-Xing, Hu Chao-Yi, Xu Bo, Hu Zhi-Gang, Zhang Chong, Sen Lin, Peng Yuan. Study on full-length coding sequence of matK gene and its coding protein from medicinal Botrychium[J]. Plant Science Journal, 2020, 38(2): 233-240. DOI: 10.11913/PSJ.2095-0837.2020.20233

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Study on full-length coding sequence of matK gene and its coding protein from medicinal Botrychium

1. College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China;
2. Institute of Gerontology, Hubei University of Chinese Medicine, Wuhan 430065, China

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This work was supported by grants from the National Natural Science Foundation of China (81574037, 31500260) and Youth Talent Project of Hubei University of Chinese Medicine (2016ZZX015).

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Received Date: November 03, 2019
Revised Date: November 20, 2019
Available Online: October 31, 2022
Published Date: April 27, 2020

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Abstract

Abstract

The matK gene plays an important role as the only group Ⅱ intros maturase in the chloroplast. We carried out bioinformatics analysis of the matK gene from five species of Botrychium. We then discussed the differences in matK gene sequences and MATK protein amino acid sequences in phylogenetic development of Botrychium. Results showed that the full length of the matK gene of the five species ranged from 1500-1503 bp, with 153 variation sites. The encoding products were all unstable hydrophilic proteins without a transmembrane structure, and the secondary structures were mainly alpha helices and random coils. Phylogenetic results showed that the phylogenetic tree constructed from the matK gene sequence was suitable for the identification of species of Botrychium. This study enriches current molecular information on matK in Botrychium and establishes the application value of matK in the identification of Botrychium.
- Botrychium,
- matK gene,
- Protein character,
- Molecular identification

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References (25)

References

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