Identification and expression analysis of the <i>DELLA</i> gene family in <i>Camellia sinensis</i> (L.) O. Ktze.

Han Yu-Xin; Dai Hong-Wei; Zheng Shu-Ting; Tong Hua-Rong; Yuan Lian-Yu

doi:10.11913/PSJ.2095-0837.2020.50644

Plant Science Journal > 2020 > 38(5): 644-653. > DOI: 10.11913/PSJ.2095-0837.2020.50644 CSTR: 32231.14.PSJ.2095-0837.2020.50644

Han Yu-Xin, Dai Hong-Wei, Zheng Shu-Ting, Tong Hua-Rong, Yuan Lian-Yu. Identification and expression analysis of the DELLA gene family in Camellia sinensis (L.) O. Ktze.[J]. Plant Science Journal, 2020, 38(5): 644-653. DOI: 10.11913/PSJ.2095-0837.2020.50644

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Identification and expression analysis of the DELLA gene family in Camellia sinensis (L.) O. Ktze.

1. College of Food Science, Southwest University, Chongqing 400715, China;
2. National Demonstration Center for Experimental Food Science and Technology Education, Southwest University, Chongqing 400715, China

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This work was supported by grants from the National Natural Science Foundation of China (31400583), Natural Science Foundation Project of CQCSTC (cstc2019jcyj-msxmX0241), Chongqing's Training Program of Innovation and Entrepreneurship for Undergraduates (S202010635166), and Fundamental Research Funds for the Central Universities (XDJK2018C069).

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Received Date: January 03, 2020
Revised Date: February 10, 2020
Available Online: October 31, 2022
Published Date: October 27, 2020

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Abstract

Abstract

Using bioinformatics methods, DELLA protein family members were analyzed from Camellia sinensis (L.) O. Ktze. genome-wide database, and their phylogenetic relationships, protein sequence characteristics, gene expression specificity, and correlation with secondary metabolites of C. sinensis were analyzed. Results identified five DELLA genes in C. sinensis: i.e., TEA009882 (CsGAI), TEA022818(CsRGA1), TEA010112(CsRGL1), TEA008736(CsRGL2), and TEA020933(CsRGL3). The number of amino acids encoded by DELLA genes ranged from 525-594 aa, all of which were located in the nucleus. Results also showed a large number of α helices and several β corners in the secondary and tertiary structures of the DELLA proteins in C. sinensis. Based on conservative domain analysis, the DELLA proteins showed high conservation in C. sinensis and Arabidopsis thaliana, with GRAS, DELLA, and other conserved motifs. Gene expression specificity showed that the expression levels of the TEA009882, TEA022818, and TEA010112 genes were high in different tissues of C. sinensis, whereas the expression levels of the TEA020933 and TEA008736 genes were very low. The expression of DELLA protein genes was regulated by abiotic stresses, such as drought, NaCl, low temperature, and methyl jasmonate (MeJA), and gene expression was correlated with the accumulation of secondary metabolites. It is speculated that DELLA genes in C. sinensis plants are widely involved in morphogenesis, abiotic stress responses, and regulation of secondary metabolite biosynthesis in C. sinensis.
- Camellia sinensis,
- DELLA proteins,
- Expression pattern,
- Bioinformatics

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References

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Identification and expression analysis of the DELLA gene family in Camellia sinensis (L.) O. Ktze.

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