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Feng Hui-Xia, Chen Gui-Lin, Guo Ming-Quan. Optimization of the extraction process of total alkaloids in Toddalia asiatica (L.) Lam. by response surface methodology[J]. Plant Science Journal, 2020, 38(6): 831-838. DOI: 10.11913/PSJ.2095-0837.2020.60831
Citation: Feng Hui-Xia, Chen Gui-Lin, Guo Ming-Quan. Optimization of the extraction process of total alkaloids in Toddalia asiatica (L.) Lam. by response surface methodology[J]. Plant Science Journal, 2020, 38(6): 831-838. DOI: 10.11913/PSJ.2095-0837.2020.60831
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Optimization of the extraction process of total alkaloids in Toddalia asiatica (L.) Lam. by response surface methodology

  • 1. CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;

  • 2. Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 437004, China;

  • 3. Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China;

  • 4. University of Chinese Academy of Sciences, Beijing 100049, China

Funds: 

This work was supported by grants from the Major Project for Special Technology Innovation of Hubei Province (2017AHB054), Natural Science Foundation of Hubei Province (2019CFB254), and Youth Innovation Promotion Association of the Chinese Academy of Sciences (2020337).

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  • Received Date: May 10, 2020
  • Revised Date: July 06, 2020
  • Available Online: October 31, 2022
  • Published Date: December 27, 2020
    Graphical Abstract
    • Abstract
  • In this study, the root of Toddalia asiatica (L.) Lam. was used as the experimental material and the content of total alkaloids was considered as the detection index. The optimum extraction conditions of total alkaloids in T. asiatica roots were investigated using response surface methodology (RSM). The optimum extraction conditions were: ethanol concentration of 74.76%, material-solvent ratio of 20∶1, and extraction time of 32.87 min, with a theoretical total alkaloid content of 11.09 mg/g. According to the optimum extraction conditions, the average value of three parallel experiments was (10.74 ±0.18) mg/g, which showed little difference from the theoretical content. Thus, the optimum extraction conditions of total alkaloids in T. asiatica obtained by the RSM were accurate and reliable, and this mathematical model was well fitted and of practical value.
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    • References (19)
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