| Citation: |
Zhan J，Zhang D，Deng K，Zhou YF，Meng B，Deng Y. Effects of different ammonium-nitrate ratios on growth and nitrogen assimilation of Saccharum officinarum L. ‘Yunzhe05-51’ at the seedling stage[J]. Plant Science Journal，2023，41（3）：349−357. DOI: 10.11913/PSJ.2095-0837.22193
|
The aim of this study was to reveal nitrogen (N) preference and optimal ammonium-nitrate ratio for growth of sugarcane (Saccharum officinarum L.). A hydroponic experiment was conducted with cultivar ‘Yunzhe05-51’ grown under a total N supply of 10 mmol/L, with seven ammonium-nitrate ratios, i.e., 0∶100, 20∶80, 40∶60, 50∶50, 60∶40, 80∶20, and 100∶0. Plant growth and physiological responses in relation to N assimilation were characterized. Results showed that the plant biomass of ‘Yunzhe05-51’ peaked at an ammonium-nitrate ratio of 20∶80. Under this treatment, plants showed the best root morphology, highest N assimilation-related enzyme activity, and relatively higher N uptake and utilization efficiency. Root growth began to display depression when the ammonium-nitrate ratio reached 40∶60, while shoot growth was significantly limited when the ammonium-nitrate ratio increased to 80∶20. Plants under single ammonium supply had the highest levels of N concentration, N uptake per unit root length, free amino acid content, and soluble protein content, but showed lower N assimilation-related enzyme activity and the lowest N utilization efficiency, as well as the most stunted plant growth and root morphology. In conclusion, exploring the combined effects of ammonium and nitrate on plant growth, N assimilation and utilization were better than single administration of either ammonium or nitrate, and the optimal ammonium-nitrate ratio for ‘Yunzhe05-51’ was 20∶80.
| [1] |
杨云川,张会娅,程禹灏,廖丽萍,杨家祯,等. 基于DSSAT-Canegro模型的广西来宾市甘蔗生长对气象干旱的响应[J]. 农业工程学报,2022,38(2):119−130.
Yang YC,Zhang HY,Cheng YH,Liao LP,Yang JZ,et al. Effects of meteorological drought on sugarcane growth using DSSAT-Canegro model in Laibin,Guangxi of China[J]. Transactions of the Chinese Society of Agricultural Engineering,2022,38 (2):119−130.
|
| [2] |
许霞,苟永刚,罗莎莎,王宇姝,余玲玲,王建武. 减量施氮对甘蔗-大豆间作系统产量稳定性的影响[J]. 热带作物学报,2020,41(7):1354−1365.
Xu X,Gou YG,Luo SS,Wang YS,Yu LL,Wang JW. Effect of nitrogen reduction on yield stability of sugarcane-soybean intercropping system[J]. Chinese Journal of Tropical Crops,2020,41 (7):1354−1365.
|
| [3] |
Hajari E,Snyman SJ,Watt MP. The effect of form and level of inorganic N on nitrogen use efficiency of sugarcane grown in pots[J]. J Plant Nutr,2017,40 (2):248−257. doi: 10.1080/01904167.2016.1237648
|
| [4] |
李进,段婷婷,邓凯,景占鑫,蔺中,等. 不同品种甘蔗生长对氮肥的响应与利用[J]. 热带作物学报,2019,40(4):649−656.
Li J,Duan TT,Deng K,Jing ZX,Lin Z,et al. Responses of sugarcane varieties growth to nitrogen fertilizer application and related use efficiency[J]. Chinese Journal of Tropical Crops,2019,40 (4):649−656.
|
| [5] |
宗桦. 中澳甘蔗产业中氮肥利用率(FNUE)的研究现状与比较分析[J]. 世界农业,2017(1):185−193.
|
| [6] |
王继华,商贺阳,杨少海. 我国甘蔗养分高效利用的研究进展[J]. 中国糖料,2018,40(6):66−68,72.
Wang JH,Shang HY,Yang SH. Research progress on nutrient efficient utilization of sugarcane in China[J]. Sugar Crops of China,2018,40 (6):66−68,72.
|
| [7] |
唐伟杰,官春云,林良斌,李丽萍,张振华,等. 不同硝铵比对油菜生长、生理与产量的影响[J]. 植物营养与肥料学报,2018,24(5):1338−1348.
Tang WJ,Guan CY,Lin LB,Li LP,Zhang ZH,et al. Effects of nitrate and ammonium supply ratios on growth,physiology and yield of oilseed rape (Brassica napus L. )[J]. Plant Nutrition and Fertilizer Science,2018,24 (5):1338−1348.
|
| [8] |
Li SX,Wang ZH,Stewart BA. Responses of crop plants to ammonium and nitrate N[J]. Adv Agron,2013,118:205−397.
|
| [9] |
程谊,张金波,蔡祖聪. 气候-土壤-作物之间氮形态契合在氮肥管理中的关键作用[J]. 土壤学报,2019,56(3):507−515.
Cheng Y,Zhang JB,Cai ZC. Key role of matching of crop-specific N preference,soil N transformation and climate conditions in soil N nutrient management[J]. Acta Pedologica Sinica,2019,56 (3):507−515.
|
| [10] |
Che J,Zhao XQ,Zhou X,Jia ZJ,Shen RF. High pH-enhanced soil nitrification was associated with ammonia-oxidizing bacteria rather than archaea in acidic soils[J]. Appl Soil Ecol,2015,85:21−29. doi: 10.1016/j.apsoil.2014.09.003
|
| [11] |
Robinson N,Brackin R,Vinall K,Soper F,Holst J,et al. Nitrate paradigm does not hold up for sugarcane[J]. PLoS One,2011,6:e19045. doi: 10.1371/journal.pone.0019045
|
| [12] |
Boschiero BN,Mariano E,Azevedo RA,Trivelin PCO. Influence of nitrate-ammonium ratio on the growth,nutrition,and metabolism of sugarcane[J]. Plant Physiol Biochem,2019,139:246−255. doi: 10.1016/j.plaphy.2019.03.024
|
| [13] |
Pissolato MD,Silveira NM,Machado EC,Zambrosi FCB,Sodek L,Ribeiro RV. Photosynthesis and biomass accumulation in young sugarcane plants grown under increasing ammonium supply in nutrient solution[J]. Theor Exp Plant Physiol,2019,31 (3):401−411. doi: 10.1007/s40626-019-00154-w
|
| [14] |
Hajari E,Snyman SJ,Watt MP. Nitrogen use efficiency of sugarcane (Saccharum spp. ) varieties under in vitro conditions with varied N supply[J]. Plant Cell Tiss Org Cult,2015,122 (1):21−29. doi: 10.1007/s11240-015-0746-y
|
| [15] |
鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000: 264−267.
|
| [16] |
De Armas R,Valadier MH,Champigny ML,Lamaze T. Influence of ammonium and nitrate on the growth and photosynthesis of sugarcane[J]. J Plant Physiol,1992,140 (5):531−535. doi: 10.1016/S0176-1617(11)80783-2
|
| [17] |
韩庆芬,陈海飞,张振华. 不同生态型拟南芥耐铵毒害差异的生理机制[J]. 植物营养与肥料学报,2019,25(7):1185−1193.
Han QF,Chen HF,Zhang ZH. Physiological mechanisms of tolerance to ammonium toxicity in different ecotypes of Arabidopsis thaliana[J]. Plant Nutrition and Fertilizer Science,2019,25 (7):1185−1193.
|
| [18] |
Brackin R,Näsholm T,Robinson N,Guillou S,Vinall K,et al. Nitrogen fluxes at the root-soil interface show a mismatch of nitrogen fertilizer supply and sugarcane root uptake capacity[J]. Sci Rep,2015,5:15727. doi: 10.1038/srep15727
|
| [19] |
Boschiero BN,Mariano E,Trivelin PCO. “Preferential” ammonium uptake by sugarcane does not increase the 15N recovery of fertilizer sources[J]. Plant Soil,2018,429 (1):253−269.
|
| [20] |
宋文静,宋科,董建新,李永亮,况帅,等. 铵硝混合营养对烟草苗期氮代谢酶及内源生长素的影响[J]. 江苏农业科学,2019,47(8):100−104. doi: 10.15889/j.issn.1002-1302.2019.08.023
|
| [21] |
屈亚潭,高欣,冯晓东. 不同铵硝配比对番茄苗期生长和光合特性的影响[J]. 延安大学学报(自然科学版),2021,40(3):16−20.
Qu YT,Gao X,Feng XD. Effects of different ammonium-nitrate ratios on growth and photosynthesis of tomato seedlings[J]. Journal of Yan’an University (Natural Science Edition)
|
| [22] |
Van Bueren LET,Struik PC. Diverse concepts of breeding for nitrogen use efficiency[J]. Agron Sustain Dev,2017,37 (5):50. doi: 10.1007/s13593-017-0457-3
|
| [23] |
Ortigosa F,Valderrama-Martín JM,Urbano-Gámez JA,García-Martín ML,Ávila C,et al. Inorganic nitrogen form determines nutrient allocation and metabolic responses in Maritime Pine seedlings[J]. Plants (Basel)
|
| [24] |
孙敏红,吴炼,谢深喜. 铵硝营养对枳橙幼苗谷氨酰胺合成酶活性及相关基因表达的影响[J]. 植物生理学报,2018,54(11):1703−1710. doi: 10.13592/j.cnki.ppj.2018.0232
Sun MH,Wu L,Xie SX. Effect of nitrate and ammonium nutrition on glutamine synthetase activities and relative genes for citrange[J]. Plant Physiology Journal,2018,54 (11):1703−1710. doi: 10.13592/j.cnki.ppj.2018.0232
|
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: