Advance Search
Du JL,Lin XL,Ma YW,Chen JR,Chen HX,Li YF. Research progress in plant Trehalose-6-phosphate synthase genes[J]. Plant Science Journal,2023,41(3):411−420. DOI: 10.11913/PSJ.2095-0837.22198
Citation: Du JL,Lin XL,Ma YW,Chen JR,Chen HX,Li YF. Research progress in plant Trehalose-6-phosphate synthase genes[J]. Plant Science Journal,2023,41(3):411−420. DOI: 10.11913/PSJ.2095-0837.22198

Research progress in plant Trehalose-6-phosphate synthase genes

Funds: This work was supported by grants from the National Key Research and Development Program Project of China (2018YFD1000400, 2020YFD1001104)
More Information
  • Received Date: October 05, 2022
  • Revised Date: November 04, 2022
  • Available Online: July 02, 2023
  • The trehalose-6-phosphate metabolic pathway is an important component of the plant physiological regulation network in response to abiotic stress. Trehalose-6-phosphate synthase (TPS) is a key gene for trehalose synthesis in plants. To better understand the role of the TPS gene in plant response to abiotic stress and regulation of flowering time, this study presents an overview of current information on the phylogenetics and sequence structure of plant TPS gene family members, as well as the regulatory function and molecular mechanism of TPS in plant responses to drought, salt damage, high temperature, and low temperature stress, and research progress regarding its effects on plant flowering regulation. This study presents a comprehensive summary of research progress on the TPS gene in response to abiotic stress and flowering regulation, while also providing future research directions and potential applications of TPS homologs.

  • [1]
    Lunn JE,Delorge I,Figueroa CM,Van Dijck P,Stitt M. Trehalose metabolism in plants[J]. Plant J,2014,79 (4):544−567. doi: 10.1111/tpj.12509
    [2]
    Acosta-Pérez P,Camacho-Zamora BD,Espinoza-Sánchez EA,Gutiérrez-Soto G,Zavala-García F,et al. Characterization of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase genes and analysis of its differential expression in maize (Zea mays) seedlings under drought stress[J]. Plants,2020,9 (3):315. doi: 10.3390/plants9030315
    [3]
    Yatsyshyn VY,Kvasko AY,Yemets AI. Genetic approaches in research on the role of trehalose in plants[J]. Cytol Genet,2017,51 (5):371−383. doi: 10.3103/S0095452717050127
    [4]
    Tang B,Wang S,Wang SG,Wang HJ,Zhang JY,Cui SY. Invertebrate trehalose-6-phosphate synthase gene:genetic architecture,biochemistry,physiological function,and potential applications[J]. Front Physiol,2018,9:30. doi: 10.3389/fphys.2018.00030
    [5]
    Wang PL,Lei XJ,Lü JX,Gao CQ. Overexpression of the ThTPS gene enhanced salt and osmotic stress tolerance in Tamarix hispida[J]. J For Res,2022,33 (1):299−308. doi: 10.1007/s11676-020-01224-5
    [6]
    Ma C,Wang ZQ,Kong BB,Lin TB. Exogenous trehalose differentially modulate antioxidant defense system in wheat callus during water deficit and subsequent recovery[J]. Plant Growth Regul,2013,70 (3):275−285. doi: 10.1007/s10725-013-9799-2
    [7]
    Vandesteene L,López-Galvis L,Vanneste K,Feil R,Maere S,et al. Expansive evolution of the TREHALOSE-6-PHOSPHATE PHOSPHATASE gene family in Arabidopsis[J]. Plant Physiol,2012,160 (2):884−896. doi: 10.1104/pp.112.201400
    [8]
    Fichtner F,Lunn JE. The role of trehalose 6-phosphate (Tre6P) in plant metabolism and development[J]. Annu Rev Plant Biol,2021,72:737−760. doi: 10.1146/annurev-arplant-050718-095929
    [9]
    Wahl V,Ponnu J,Schlereth A,Arrivault S,Langenecker T,et al. Regulation of flowering by trehalose-6-phosphate signaling in Arabidopsis thaliana[J]. Science,2013,339 (6120):704−707. doi: 10.1126/science.1230406
    [10]
    Du LS,Qi SY,Ma JJ,Xing LB,Fan S,et al. Identification of TPS family members in apple (Malus x domestica Borkh.) and the effect of sucrose sprays on TPS expression and floral induction[J]. Plant Physiol Biochem,2017,120:10−23. doi: 10.1016/j.plaphy.2017.09.015
    [11]
    De Virgilio C,Bürckert N,Bell W,Jeno P,Boller T,Wiemken A. Disruption of TPS2,the gene encoding the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae,causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity[J]. Eur J Biochem,1993,212 (2):315−323. doi: 10.1111/j.1432-1033.1993.tb17664.x
    [12]
    Kaasen I,Falkenberg P,Styrvold OB,Strøm AR. Molecular cloning and physical mapping of the otsBA genes,which encode the osmoregulatory trehalose pathway of Escherichia coli:evidence that transcription is activated by katF (AppR)[J]. J Bacteriol,1992,174 (3):889−898. doi: 10.1128/jb.174.3.889-898.1992
    [13]
    Kaasen I,McDougall J,Strøm AR. Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex[J]. Gene,1994,145 (1):9−15. doi: 10.1016/0378-1119(94)90316-6
    [14]
    Blázquez MA,Santos E,Flores CL,Martinez-Zapater JM,Salinas J,Gancedo C. Isolation and molecular characterization of the Arabidopsis TPS1 gene,encoding trehalose-6-phosphate synthase[J]. Plant J,1998,13 (5):685−689. doi: 10.1046/j.1365-313X.1998.00063.x
    [15]
    Delorge I,Janiak M,Carpentier S,van Dijck P. Fine tuning of trehalose biosynthesis and hydrolysis as novel tools for the generation of abiotic stress tolerant plants[J]. Front Plant Sci,2014,5:147.
    [16]
    Delorge I,Figueroa CM,Feil R,Lunn JE,van Dijick P. Trehalose-6-phosphate synthase 1 is not the only active TPS in Arabidopsis thaliana[J]. Biochem J,2015,466 (2):283−290. doi: 10.1042/BJ20141322
    [17]
    Lunn JE. Gene families and evolution of trehalose metabolism in plants[J]. Funct Plant Biol,2007,34 (6):550−563. doi: 10.1071/FP06315
    [18]
    Ramon M,De Smet I,Vandesteene L,Naudts M,Leyman B,et al. Extensive expression regulation and lack of heterologous enzymatic activity of the Class Ⅱ trehalose metabolism proteins from Arabidopsis thaliana[J]. Plant Cell Environ,2009,32 (8):1015−1032. doi: 10.1111/j.1365-3040.2009.01985.x
    [19]
    Chary SN,Hicks GR,Choi YG,Carter D,Raikhel NV. Trehalose-6-phosphate synthase/phosphatase regulates cell shape and plant architecture in Arabidopsis[J]. Plant Physiol,2008,146 (1):97−107. doi: 10.1104/pp.107.107441
    [20]
    Suzuki N,Bajad S,Shuman J,Shulaev V,Mittler R. The transcriptional co-activator MBF1c is a key regulator of thermotolerance in Arabidopsis thaliana[J]. J Biol Chem,2008,283 (14):9269−9275. doi: 10.1074/jbc.M709187200
    [21]
    Lin MF,Jia RH,Li JC,Zhang MJ,Chen HB,et al. Evolution and expression patterns of the trehalose-6-phosphate synthase gene family in drumstick tree (Moringa oleifera Lam.)[J]. Planta,2018,248 (4):999−1015. doi: 10.1007/s00425-018-2945-3
    [22]
    Zang BS,Li HW,Li WJ,Deng XW,Wang XP. Analysis of trehalose-6-phosphate synthase (TPS) gene family suggests the formation of TPS complexes in rice[J]. Plant Mol Biol,2011,76 (6):507−522. doi: 10.1007/s11103-011-9781-1
    [23]
    蒋伟. 玉米海藻糖-6-磷酸合成酶基因家族的功能验证和差异表达分析[D]. 雅安: 四川农业大学, 2010: 32−34.
    [24]
    谢翎,汪章勋,黄勃. 大豆TPS基因家族全基因组鉴定、分类与表达分析[J]. 中国油料作物学报,2014,36(2):160−167. doi: 10.7505/j.issn.1007-9084.2014.02.004

    Xie L,Wang ZX,Huang B. Genome-wide identification classification and expression of TPS family genes in soybean[J]. Chinese Journal of Oil Crop Sciences,2014,36 (2):160−167. doi: 10.7505/j.issn.1007-9084.2014.02.004
    [25]
    Gao YH,Yang XY,Yang X,Zhao TY,An XM,Chen Z. Characterization and expression pattern of the trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase gene families in Populus[J]. Int J Biol Macromol,2021,187:9−23. doi: 10.1016/j.ijbiomac.2021.07.096
    [26]
    Xie DW,Wang XN,Fu LS,Sun J,Zheng W,Li ZF. Identification of the trehalose-6-phosphate synthase gene family in winter wheat and expression analysis under conditions of freezing stress[J]. J Genet,2015,94 (1):55−65. doi: 10.1007/s12041-015-0495-z
    [27]
    Jin QJ,Hu X,Li X,Wang B,Wang YJ,et al. Genome-wide identification and evolution analysis of trehalose-6-phosphate synthase gene family in Nelumbo nucifera[J]. Front Plant Sci,2016,7:1445.
    [28]
    Xu YC,Wang YJ,Mattson N,Yang L,Jin QJ. Genome-wide analysis of the Solanum tuberosum (potato) trehalose-6-phosphate synthase (TPS) gene family:evolution and differential expression during development and stress[J]. BMC Genomics,2017,18 (1):926. doi: 10.1186/s12864-017-4298-x
    [29]
    Hu X,Wu ZD,Luo ZY,Burner DM,Pan YB,Wu CW. Genome-wide analysis of the trehalose-6-phosphate synthase (TPS) gene family and expression profiling of ScTPS genes in sugarcane[J]. Agronomy,2020,10 (7):969. doi: 10.3390/agronomy10070969
    [30]
    Dan YY,Niu Y,Wang CL,Yan M,Liao WB. Genome-wide identification and expression analysis of the trehalose-6-phosphate synthase (TPS) gene family in cucumber (Cucumis sativus L.)[J]. PeerJ,2021,9:e11398. doi: 10.7717/peerj.11398
    [31]
    Li YF,Zhang MF,Zhang M,Jia GX. Analysis of global gene expression profiles during the flowering initiation process of Lilium × formolongi[J]. Plant Mol Biol,2017,94 (4-5):361−379. doi: 10.1007/s11103-017-0612-x
    [32]
    Sun J,Dai ZG,Zhang XY,Tang Q,Cheng CH,et al. Identification of TPS and TPP gene families in Cannabis sativa and their expression under abiotic stresses[J]. Biol Plant,2022,66:14−23. doi: 10.32615/bp.2021.051
    [33]
    Kosmas SA,Argyrokastritis A,Loukas MG,Eliopoulos E,Tsakas S,Kaltsikes PJ. Isolation and characterization of drought-related trehalose 6-phosphate-synthase gene from cultivated cotton (Gossypium hirsutum L.)[J]. Planta,2006,223 (2):329−339. doi: 10.1007/s00425-005-0071-5
    [34]
    Morabito C,Secchi F,Schubert A. Grapevine TPS (trehalose-6-phosphate synthase) family genes are differentially regulated during development,upon sugar treatment and drought stress[J]. Plant Physiol Biochem,2021,164:54−62. doi: 10.1016/j.plaphy.2021.04.032
    [35]
    Yang YJ,Ma KF,Zhang TX,Li LL,Wang J,et al. Characteristics and expression analyses of trehalose-6-phosphate synthase family in Prunus mume reveal genes involved in trehalose biosynthesis and drought response[J]. Biomolecules,2020,10 (10):1358. doi: 10.3390/biom10101358
    [36]
    Yeo ET,Kwon HB,Han SE,Lee JT,Ryu JC,Byu MO. Genetic engineering of drought resistant potato plants by introduction of the trehalose-6-phosphate synthase (TPS1) gene from Saccharomyces cerevisiae[J]. Mol Cells,2000,10 (3):263−268.
    [37]
    Li HW,Zang BS,Deng XW,Wang XP. Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice[J]. Planta,2011,234 (5):1007−1018. doi: 10.1007/s00425-011-1458-0
    [38]
    Han BY,Fu LL,Zhang D,He XQ,Chen Q,et al. Interspecies and intraspecies analysis of trehalose contents and the biosynthesis pathway gene family reveals crucial roles of trehalose in osmotic-stress tolerance in cassava[J]. Int J Mol Sci,2016,17 (7):1077. doi: 10.3390/ijms17071077
    [39]
    Stiller I,Dulai S,Kondrák M,Tarnai R,Szabó L,et al. Effects of drought on water content and photosynthetic parameters in potato plants expressing the trehalose-6-phosphate synthase gene of Saccharomyces cerevisiae[J]. Planta,2008,227 (2):299−308.
    [40]
    Liu YB,Han LZ,Qin LJ,Zhao DG. Saccharomyces cerevisiae gene TPS1 improves drought tolerance in Zea mays L. by increasing the expression of SDD1 and reducing stomatal density[J]. Plant Cell Tissue Organ Cult,2015,120 (2):779−789. doi: 10.1007/s11240-014-0647-5
    [41]
    El-Bashiti T,Hamamcı H,Öktem HA,Yücel M. Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions[J]. Plant Sci,2005,169 (1):47−54. doi: 10.1016/j.plantsci.2005.02.024
    [42]
    Song JB,Mao HY,Cheng J,Zhou Y,Chen RR,et al. Identification of the trehalose-6-phosphate synthase gene family in Medicago truncatula and expression analysis under abiotic stresses[J]. Gene,2021,787:145641. doi: 10.1016/j.gene.2021.145641
    [43]
    Almeida AM,Villalobos E,Araújo SS,Leyman B,van Dijck P,et al. Transformation of tobacco with an Arabidopsis thaliana gene involved in trehalose biosynthesis increases tolerance to several abiotic stresses[J]. Euphytica,2005,146 (1-2):165−176. doi: 10.1007/s10681-005-7080-0
    [44]
    Vishal B,Krishnamurthy P,Ramamoorthy R,Kumar PP. OsTPS8 controls yield-related traits and confers salt stress tolerance in rice by enhancing suberin deposition[J]. New Phytol,2019,221 (3):1369−1386. doi: 10.1111/nph.15464
    [45]
    Jiang T,Zhai H,Wang FB,Zhou HN,Si ZZ,et al. Cloning and characterization of a salt tolerance-associated gene encoding trehalose-6-phosphate synthase in sweetpotato[J]. J Integr Agric,2014,13 (8):1651−1661. doi: 10.1016/S2095-3119(13)60534-1
    [46]
    Yuan GP,Liu JP,An GL,Li WH,Si WJ,et al. Genome-wide identification and characterization of the trehalose-6-phosphate synthetase (TPS) gene family in watermelon (Citrullus lanatus) and their transcriptional responses to salt stress[J]. Int J Mol Sci,2021,23 (1):276. doi: 10.3390/ijms23010276
    [47]
    Miranda JA,Avonce N,Suárez R,Thevelein JM,van Dijck P,Iturriaga G. A bifunctional TPS-TPP enzyme from yeast confers tolerance to multiple and extreme abiotic-stress conditions in transgenic Arabidopsis[J]. Planta,2007,226 (6):1411−1421. doi: 10.1007/s00425-007-0579-y
    [48]
    Lyu JI,Park JH,Kim JK,Bae CH,Jeong WJ,et al. Enhanced tolerance to heat stress in transgenic tomato seeds and seedlings overexpressing a trehalose-6-phosphate synthase/phosphatase fusion gene[J]. Plant Biotechnol Rep,2018,12 (6):399−408. doi: 10.1007/s11816-018-0505-8
    [49]
    Mollavali M,Börnke F. Characterization of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase genes of tomato (Solanum lycopersicum L.) and analysis of their differential expression in response to temperature[J]. Int J Mol Sci,2022,23 (19):11436. doi: 10.3390/ijms231911436
    [50]
    庞强强,蔡兴来,孙晓东,张文,周曼. 大白菜TPS基因家族鉴定及其在高温胁迫下的表达分析[J]. 分子植物育种,2020,18(8):2452−2459.

    Pang QQ,Cai XL,Sun XD,Zhang W,Zhou M. Identification of TPS gene family in Chinese cabbage (Brassica campestris L.) and its expression under heat stress[J]. Molecular Plant Breeding,2020,18 (8):2452−2459.
    [51]
    谢冬微. 冬小麦低温下基因表达谱分析及海藻糖基因家族研究[D]. 哈尔滨: 东北农业大学, 2014: 77−83.
    [52]
    丁菲,庞磊,李叶云,葛菁,江昌俊. 茶树海藻糖-6-磷酸合成酶基因(CsTPS)的克隆及表达分析[J]. 农业生物技术学报,2012,20(11):1253−1261.

    Ding F,Pang L,Li YY,Ge J,Jiang CJ. Cloning and expression analysis of trehalose-6-phosphate synthase gene(CsTPS) from tea plant (Camellia sinensis (L. ) O. Kuntz)[J]. Journal of Agricultural Biotechnology,2012,20 (11):1253−1261.
    [53]
    Liu X,Fu LS,Qin P,Sun YL,Liu J,Wang XN. Overexpression of the wheat trehalose 6-phosphate synthase 11 gene enhances cold tolerance in Arabidopsis thaliana[J]. Gene,2019,710:210−217. doi: 10.1016/j.gene.2019.06.006
    [54]
    Avonce N,Leyman B,Mascorro-Gallardo JO,van Dijck P,Thevelein JM,Iturriaga G. The Arabidopsis trehalose-6-P synthase AtTPS1 gene is a regulator of glucose,abscisic acid,and stress signaling[J]. Plant Physiol,2004,136 (3):3649−3659. doi: 10.1104/pp.104.052084
    [55]
    Wei XR,Ling W,Ma YW,Du JL,Cao FX,et al. Genome-wide analysis of the trehalose-6-phosphate synthase gene family in rose (Rosa chinensis) and differential expression under heat stress[J]. Horticulturae,2022,8 (5):429. doi: 10.3390/horticulturae8050429
    [56]
    Zhang Q,Zhang M,Zhao YQ,Hu H,Huang YX,Jia GX. Identification of trehalose-6-phosphate synthase (TPS)-coding genes involved in flowering induction of Lilium × formolongi[J]. Plant Physiol Biochem,2022,171:84−94. doi: 10.1016/j.plaphy.2021.12.025
    [57]
    Lin MF,Ma SY,Quan KH,Yang ED,Hu L,Chen XY. Comparative transcriptome analysis provides insight into the molecular mechanisms of long-day photoperiod in Moringa oleifera[J]. Physiol Mol Biol Plants,2022,28 (5):935−946. doi: 10.1007/s12298-022-01186-4
    [58]
    赵美丽,倪军,陈茂盛,徐增富. 超量表达小桐子JcTPS1促进拟南芥开花并增加叶片花青素含量[J]. 分子植物育种,2018,16(1):255−261.

    Zhao ML,Ni J,Chen MS,Xu ZF. Over-expression of JcTPS1 from Jatropha curcas induces early flowering of Arabidopsis and improves anthocyanin accumulation in leaves[J]. Molecular Plant Breeding,2018,16 (1):255−261.
    [59]
    陈宏艳,李小二,李忠光. 糖信号及其在植物响应逆境胁迫中的作用[J]. 生物技术通报,2022,38(7):80−89.

    Chen HY,Li XE,Li ZG. Sugar signaling and its role in plant response to environmental stress[J]. Biotechnology Bulletin,2022,38 (7):80−89.
    [60]
    Bischof S. Life is sweeter with trehalose 6-phosphate[J]. Plant Cell,2020,32 (6):1784−1785. doi: 10.1105/tpc.20.00276
    [61]
    Paul MJ,Gonzalez-Uriarte A,Griffiths CA,Hassani-Pak K. The role of trehalose 6-phosphate in crop yield and resilience[J]. Plant Physiol,2018,177 (1):12−23. doi: 10.1104/pp.17.01634
    [62]
    Saidi A,Hajibarat Z. Computational analysis and expression study of trehalose 6-phosphate synthase (TPS) in rice (Oryza sativa)[J]. Sci Study Res:Chem Chem Eng,Biotechnol,Food Ind,2021,22 (1):13−25.
    [63]
    项阳,刘延波,赵德刚,Yi Li. 转TPS1基因促进干旱胁迫条件下的花青素积累提高玉米植株抗旱性[J]. 植物生理学报,2015,51(11):2054−2062.

    Xiang Y,Liu YB,Zhao DG,Yi L. Improve drought tolerance via accumulating anthocyanidin under drought stress in TPS1 transgenic maize[J]. Plant Physiology Journal,2015,51 (11):2054−2062.
    [64]
    Krishnamurthy P,Jyothi-Prakash PA,Qin L,He J,Lin QS,et al. Role of root hydrophobic barriers in salt exclusion of a mangrove plant Avicennia officinalis[J]. Plant Cell Environ,2014,37 (7):1656−1671. doi: 10.1111/pce.12272
    [65]
    Gabriel C,Fernhout JJ,Fichtner F,Feil R,Lunn JE,et al. Genetic manipulation of trehalose-6-phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems[J]. Plant Direct,2021,5 (11):e358.
    [66]
    Romero C,Bellés JM,Vayá JL,Serrano R,Culiáñez-Macià FA. Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants:pleiotropic phenotypes include drought tolerance[J]. Planta,1997,201 (3):293−297. doi: 10.1007/s004250050069
    [67]
    Garg AK,Kim JK,Owens TG,Ranwala AP,Choi YD,et al. Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses[J]. Proc Natl Acad Sci USA,2002,99 (25):15898−15903. doi: 10.1073/pnas.252637799
    [68]
    杜丽璞,徐惠君,叶兴国,林忠平. 小麦转TPS基因植株的获得及其初步功能鉴定[J]. 麦类作物学报,2007,27(3):369−373. doi: 10.3969/j.issn.1009-1041.2007.03.001

    Du LP,Xu HJ,Ye XG,Lin ZP. Transgenic wheat plants with trehalose-6-phosphate synthase (TPS) gene and identification of their function[J]. Journal of Triticeae Crops,2007,27 (3):369−373. doi: 10.3969/j.issn.1009-1041.2007.03.001
    [69]
    程庚. 垫状卷柏海藻糖-6-磷酸合成酶基因转化玉米[D]. 雅安: 四川农业大学, 2012: 31−37.
  • Related Articles

    [1]Wang Wan-Wan, Lu Zhen-Wei, Yuan Long-Yi, Jiang Hong-Sheng. Effects of silver nanoparticles on dormant bud germination, seedling survival, and growth of Spirodela polyrhiza (L.) Schleid[J]. Plant Science Journal, 2022, 40(4): 576-583. DOI: 10.11913/PSJ.2095-0837.2022.40576
    [2]Kong Ling-Pu, Zhu Yong-Jie, Qiu Mei, Wang Huan-Chong, He Zhao-Rong. Effects of physical and chemical factors on seed germination of Bupleurum dracaenoides[J]. Plant Science Journal, 2017, 35(3): 421-426. DOI: 10.11913/PSJ.2095-0837.2017.30421
    [3]WANG Xue-Jing, BU Hai-Yan, ZHOU Xian-Hui, XU Dang-Hui, LIU Wei, QI Wei, GE Wen-Jing. Effect of Seed Shape on Germination in an Alpine Meadow on the Eastern Qinghai-Tibet Plateau[J]. Plant Science Journal, 2016, 34(3): 391-396. DOI: 10.11913/PSJ.2095-0837.2016.30391
    [4]PU Yun-Hai, LIU Gui-Hua, LI Wei. Seed Germination of Potamogeton malaianus[J]. Plant Science Journal, 2005, 23(2): 179-182.
    [5]HONG Lan, SHEN Hao, YANG Qi-He, CAO Hong-Lin, YE Wan-Hui. Studies on Seed Germination and Storage of the Invasive Alien Species Bidens pilosa L.[J]. Plant Science Journal, 2004, 22(5): 433-437.
    [6]ZHOU Yuan, SUN Wei-Bang, Li Cong-Ren. Preliminary Study on Germination of Trigonobalanus doichangensis[J]. Plant Science Journal, 2003, 21(1): 73-76.
    [7]YANG Qi-He, YIN Shou-Hua, XIA Yong-Mei, LAN Qin-Ying. Preliminary Study on Germination Capacity and Desiccation-tolerance of Cassia hirsuta Seeds at Various Developmental Stages[J]. Plant Science Journal, 2002, 20(4): 288-292.
    [8]Xu Zhenxiu, Hu Chunkui, Lan Shengyin. A TECHNIQUE OF OBSERVATION ON PARAFFIN SECTIONS WITH SCANNING ELECTRON MICROSCOPE[J]. Plant Science Journal, 1992, 10(4): 377-380.
    [9]Xia Renxue, Peng Shuang, Chen Guilin, Hu Shiquan. STUDIES ON THE FACTORS INFLUENCING POLLEN GERMINATION OF CASTANEA MOLLISSIMA BL.[J]. Plant Science Journal, 1989, 7(4): 351-355.
    [10]Wan Caigan. A STUDY ON DORMANCY AND PREGERMINATION OF THE VIBURNUM BETULIFOLIUM SEEDS[J]. Plant Science Journal, 1985, 3(2): 197-202.
  • Cited by

    Periodical cited type(3)

    1. 张萌,陈着,何瑞瑞,徐杰,聂宏. 荷叶及其有效成分治疗代谢综合征研究进展. 辽宁中医药大学学报. 2025(01): 115-120 .
    2. 罗淑芬,杨何,孙露,胡花丽,周宏胜,刘雪松,凌军,张映曈,李鹏霞. NO参与褪黑素延缓莲子采后褐变的作用机理. 农业工程学报. 2024(21): 263-273 .
    3. 阎莹莹,张陈,张文会. 西藏蕨麻黄酮组成鉴定及抗氧化能力的研究. 食品安全质量检测学报. 2023(20): 86-95 .

    Other cited types(3)

Catalog

    Article views PDF downloads Cited by(6)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return