Effects of the broken <i>rps2</i> gene cluster on evolutionary rates in Campanulaceae

Li Jin-Ye; Ping Jing-Yao; Cui Gui-Feng; Su Ying-Juan; Wang Ting

doi:10.11913/PSJ.2095-0837.22231

Plant Science Journal > 2023 > 41(3): 333-342. > DOI: 10.11913/PSJ.2095-0837.22231 CSTR: 32231.14.PSJ.2095-0837.22231

Li JY，Ping JY，Cui GF，Su YJ，Wang T. Effects of the broken rps2 gene cluster on evolutionary rates in Campanulaceae[J]. Plant Science Journal，2023，41（3）：333−342. DOI: 10.11913/PSJ.2095-0837.22231

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Effects of the broken rps2 gene cluster on evolutionary rates in Campanulaceae

1.
College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
2.
Nanzhao State - owned Forest Farms (Funiu Mountain National Nature Reserve Nanzhao Baotianman Administration in Henan), Nanzhao, Henan 474665, China
3.
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
4.
Research Institute of Sun Yat-sen University in Shenzhen, Shenzhen, Guangdong 518057, China

Funds: This work was supported by a grant from the National Natural Science Foundation of China (31770587)

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Received Date: September 02, 2022
Revised Date: November 08, 2022
Available Online: July 02, 2023

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Abstract

Abstract

The conserved rps2 gene cluster (rps2-atpI-atpH-atpF-atpA) is broken in the chloroplast genome of Campanulaceae. To explore whether the evolutionary rates of the related genes changed after the cluster was broken, we selected the chloroplast genome sequences of 50 Campanulaceae and two Menyanthaceae species to construct their phylogenetic relationships, and further analyzed the evolutionary rate, selection pressure, and adaptive evolutionary process of the rps2 gene cluster-related genes. Results showed that all species with a broken rps2 gene cluster in Campanulaceae were from Campanuloideae and formed monophyletic branches on the phylogenetic tree. Compared with the unbroken species, the mean evolutionary rate of the related genes decreased in the broken species. There were significant differences in the nonsynonymous substitution rate between genes. In adaptive evolutionary analysis, the site model detected positive-selection sites in the atpI and rps2 genes. Our findings suggest that the fragmentation of the gene cluster holds phylogenetic implications, leading to alterations in evolutionary rates and diverse evolutionary trajectories among individual genes.
- rps2 gene cluster,
- Campanulaceae,
- Evolutionary rate,
- Selection pressure,
- Adaptive evolution

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References (37)

References

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Effects of the broken rps2 gene cluster on evolutionary rates in Campanulaceae