Identification and functional analysis of <i>Euphorbia lathyris</i> L. transcription factor ElWRI1

Niu Ting-Feng; Ge Li-Ping; Su Yun-Ting; Wang Zhuang-Lin; Li Run-Zhi

doi:10.11913/PSJ.2095-0837.22250

Plant Science Journal > 2023 > 41(4): 458-466. > DOI: 10.11913/PSJ.2095-0837.22250 CSTR: 32231.14.PSJ.2095-0837.22250

Niu TF，Ge LP，Su YT，Wang ZL，Li RZ. Identification and functional analysis of Euphorbia lathyris L. transcription factor ElWRI1[J]. Plant Science Journal，2023，41（4）：458−466. DOI: 10.11913/PSJ.2095-0837.22250

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Identification and functional analysis of Euphorbia lathyris L. transcription factor ElWRI1

1.
College of Forestry, Shanxi Agricultural University, Taigu, Shanxi 030801, China
2.
Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China

Funds: This work was supported by grants from Shanxi Natural Science Foundation Project (201901D111223) and Shanxi Province Graduate Education Innovation Project (J202282045).

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Received Date: November 20, 2022
Revised Date: December 23, 2022
Available Online: September 06, 2023

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Abstract

Abstract

Seeds of Euphorbia lathyris L. accumulate a substantial amount of oil enriched with oleic acid. The plant transcription factor WRINKLED1 (WRI1) is a member of the AP2/EREBP transcription factor family, which is involved in the regulation of fatty acid synthesis and seed development. Based on the whole genome data of E. lathyris, 11 ElWRI1 genes were identified, namely ElWRI1-1–ElWRI1-11, respectively. All identified ElWRI1s contained two AP2 domains. The ElWRI1 proteins were unstable hydrophilic proteins, lacking a transmembrane structure. The 11 ElWRI1 proteins were predicted to localize in the nucleus. Heatmap analysis revealed that ElWRI1-6 and ElWRI1-8 exhibited higher expression levels, with the expression pattern of ElWRI1-8 mirroring that of seed oil accumulation. Heterologous overexpression of ElWRI1-8 in Nicotiana benthamiana L. leaf tissues led to a significant increase in total oil and oleic acid contents in the leaf. This study indicates that ElWRI1-8 is an important transcription factor in regulating biosynthesis of oil and oleic acid in E. lathyris seeds, thus providing a new scientific basis for the high-yield and high-quality breeding of E. lathyris and the development of high-value oil products.
- Euphorbia lathyris,
- Transcription factors,
- WRI1,
- Oil synthesis,
- Leaf transient expression

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References (23)

References

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Identification and functional analysis of Euphorbia lathyris L. transcription factor ElWRI1