Regulatory effects of fertilization on root morphology and needle physiological characteristics of <i>Pinus armandii</i> Franch. container seedlings

Feng Wei; Chen Shi; Shan Chang-Dan; Zhang Hui; Yao Zeng-Yu

doi:10.11913/PSJ.2095-0837.22283

Plant Science Journal > 2023 > 41(4): 521-530. > DOI: 10.11913/PSJ.2095-0837.22283 CSTR: 32231.14.PSJ.2095-0837.22283

Feng W，Chen S，Shan CD，Zhang H，Yao ZY. Regulatory effects of fertilization on root morphology and needle physiological characteristics of Pinus armandii Franch. container seedlings[J]. Plant Science Journal，2023，41（4）：521−530. DOI: 10.11913/PSJ.2095-0837.22283

Citation:

PDF (654 KB)

Regulatory effects of fertilization on root morphology and needle physiological characteristics of Pinus armandii Franch. container seedlings

1.
Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Southwest Forestry University, Kunming 650224, China
2.
Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China

Funds: This work was supported by a grant from the Joint Special Project on Basic Agricultural Research of Yunnan Province (2018FG001-008).

More Information

Received Date: October 26, 2022
Revised Date: December 27, 2022
Available Online: September 06, 2023

Graphical Abstract

Abstract

Abstract

This study explored the growth, root morphology, and physiological characteristics of Pinus armandii Franch. container seedlings under different fertilization treatments to reveal the regulatory effects of fertilization on seedling quality. The P. armandii container seedlings were grown under four fertilizer rates (100, 200, 300, and 400 mg N/seedling) with either conventional or exponential fertilization, with no fertilization serving as a control. The effects of fertilization on growth, root morphology, and physiological characteristics were then analyzed. Results showed that under both conventional and exponential fertilization, seedling height, root collar diameter, biomass, root length, root surface area, and root volume all initially increased and then decreased, while root tissue density and soluble sugar and malondialdehyde contents initially decreased and then increased with the increase in fertilizer application rates. Furthermore, as fertilizer application increased, specific root length and specific surface area decreased while root average diameter and free proline and soluble protein contents increased. Most indices were higher under exponential fertilization than conventional fertilization at the same fertilizer rate. The E300 treatment (exponential fertilization at 300 mg N per plant) yielded the highest comprehensive score. In conclusion, rational fertilization promoted seedling growth and root development and improved the physiology of P. armandii container seedlings, with the optimal fertilization treatment being exponential fertlization at 300 mg N per plant.
- Pinus armandii,
- Exponential fertilization,
- Seedling growth,
- Root morphology,
- Physiological characteristics

FullText(HTML)

References (31)

References

[1]	中国科学院昆明植物研究所. 云南植物志（第11卷-种子植物）[M]. 北京: 科学出版社, 2000: 83-87.
[2]	中国科学院中国植物志编辑委员会. 中国植物志: 第7卷[M]. 北京: 科学出版社, 1978: 217.
[3]	王若楠,白吉庆,王玛丽,阎晓昊,董鹏斌,等. 中国特有森林树种华山松的群体进化历史研究[J]. 西北植物学报,2019,39(1):42−51. Wang RN,Bai JQ,Wang ML,Yan XH,Dong PB,et al. Evolutionary history of an endemic conifer tree Pinus armandii in China[J]. Acta Botanica Boreali-Occidentalia Sinica,2019,39 (1):42−51.
[4]	Grossnickle SC. Why seedlings survive:influence of plant attributes[J]. New Forests,2012,43 (5):711−738.
[5]	李国雷,刘勇,祝燕,蒋乐,史文辉. 国外容器苗质量调控技术研究进展[J]. 林业科学,2012,48(8):135−142. Li GL,Liu Y,Zhu Y,Jiang L,Shi WH. A review on the abroad studies of techniques in regulating quality of container seedling[J]. Scientia Silvae Sinicae,2012,48 (8):135−142.
[6]	Ingestad T,Lund AB. Theory and techniques for steady state mineral nutrition and growth of plants[J]. Scand J Forest Res,1986,1 (1-4):439−453. doi: 10.1080/02827588609382436
[7]	Timmer VR. Exponential nutrient loading:a new fertilization technique to improve seedling performance on competitive sites[J]. New Forests,1997,13 (1-3):279−299. doi: 10.1023/A:1006502830067
[8]	Pokharel P,Kwak JH,Chang SX. Growth and nitrogen uptake of jack pine seedlings in response to exponential fertilization and weed control in reclaimed soil[J]. Biol Fertil Soils,2017,53 (6):701−713. doi: 10.1007/s00374-017-1213-1
[9]	Uscola M,Salifu KF,Oliet JA,Jacobs DF. An exponential fertilization dose−response model to promote restoration of the Mediterranean oak Quercus ilex[J]. New Forests,2015,46 (5):795−812.
[10]	Hu YL,Fan HH,Xuan HF,Mgelwa AS,Chen SP. Distinct growth and nutrient status responses to fertilization regimes in two generations of Chinese fir seedlings[J]. Forests,2019,10 (9):719. doi: 10.3390/f10090719
[11]	郝龙飞,刘婷岩,张连飞,贾斌英,李宁,李开隆. 氮素指数施肥对白桦播种苗养分承载和光合作用的影响[J]. 北京林业大学学报,2014,36(6):17−23. Hao LF,Liu TY,Zhang LF,Jia BY,Li N,Li KL. Effects of N exponential fertilization on nutrient loading and photosynthesis of Betula platyphylla seedlings[J]. Journal of Beijing Forestry University,2014,36 (6):17−23.
[12]	王益明,卢艺,张慧,鞠昌华,裴文明,等. 指数施肥对美国山核桃幼苗生长及叶片养分含量的影响[J]. 中国土壤与肥料,2018(6):136−140. Wang YM,Lu Y,Zhang H,Ju CH,Pei WM,et al. Effects of exponential fertilization on growth and foliar nutrient status of pecan seedlings[J]. Soils and Fertilizers Sciences in China,2018 (6):136−140.
[13]	闫小莉,林智熠,胡文佳,马祥庆,曲鲁平. 杉木根系形态特征及其觅养策略研究进展[J]. 世界林业研究,2022,35(1):26−31. Yan XL,Lin ZY,Hu WJ,Ma XQ,Qu LP. Root morphological characteristics of Cunninghamia lanceolata and its foraging strategies[J]. World Forestry Research,2022,35 (1):26−31.
[14]	王妍,冯金玲,吴小慧,黄蓝明,吴娟,等. 施肥对闽楠根系形态及苗木质量的调控作用[J]. 西北农林科技大学学报(自然科学版),2022,50(10):44−56. Wang Y,Feng JL,Wu XH,Huang LM,Wu J,et al. Effects of fertilization on root morphology and seedling quality of Phoebe bournei[J]. Journal of Northwest A& F University (Natural Sciences Edition),2022,50 (10):44−56.
[15]	程洁茹, 钟全林, 邹宇星, 常云妮, 廖鹏飞. 氮磷配施对刨花楠幼苗相对生长速率与细根性状关系的影响[J/OL]. 生态学杂志, 2022. https://kns.cnki.net/kcms/detail/21.1148.Q.20221008.1539.002.html. Cheng JR, Zhong QL, Zou YX, Chang YN, Liao PF. Effects of different ratios of nitrogen and phosphorus addition on the relationship between the relative growth rate and fine root traits of Machilus pauhoi seedlings[J/OL]. Chinese Journal of Ecology, 2022. https://kns.cnki.net/kcms/detail/21.1148.Q.20221008.1539.002.html.
[16]	杨阳,张德鹏,及利,张洁,杨立学. 配比施肥对紫椴播种苗生长、养分积累及根系形态的影响[J]. 中南林业科技大学学报,2021,41(9):63−70. Yang Y,Zhang DP,Ji L,Zhang J,Yang LX. Effects of formula fertilization on growth,nutrient accumulation and root morphology of Tilia amurensis seedlings[J]. Journal of Central South University of Forestry & Technology,2021,41 (9):63−70.
[17]	Wang GL,Liu F,Xue S. Nitrogen addition enhanced water uptake by affecting fine root morphology and coarse root anatomy of Chinese pine seedlings[J]. Plant Soil,2017,418 (1):177−189.
[18]	Ni M,Gao ZZ,Chen H,Chen C,Yu FY. Exponential fertilization regimes improved growth and nutrient status of Quercus nuttallii container seedlings[J]. Agronomy,2022,12 (3):669. doi: 10.3390/agronomy12030669
[19]	荣俊冬,凡莉莉,陈礼光,张迎辉,何天友,等. 不同施氮模式和施氮量对福建柏幼苗生物量分配和根系生长的影响[J]. 林业科学,2020,56(7):175−184. Rong JD,Fan LL,Chen LG,Zhang YH,He TY,et al. Impacts on biomass allocation and root growth of Fokienia hodginsii seedlings of different patterns and quantities of nitrogen application[J]. Scientia Silvae Sinicae,2020,56 (7):175−184.
[20]	周樊,陈文静,曹凡,冯刚,李永荣,彭方仁. 配比施肥对薄壳山核桃幼苗生长和生理特性的影响[J]. 中南林业科技大学学报,2020,40(9):96−103. Zhou F,Chen WJ,Cao F,Feng G,Li YR,Peng FR. Effects of fertilization on growth and physiological characteristics of pecan seedlings[J]. Journal of Central South University of Forestry & Technology,2020,40 (9):96−103.
[21]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000: 17-90.
[22]	马道承, 余注光, 王凌晖, 林泳志, 潘媛媛. 氮磷钾配比施肥对赤苍藤生理及生物量积累的影响[J]. 植物科学学报, 2022, 40（6）: 839-852. Ma DC, Yu ZG, Wang LH, Lin YZ, Pan YY. Effects of nitrogen-phosphorus-potassium ratio fertilization on physiology and biomass accumulation of Erythropalum scandens Bl. [J]. Plant Science Journal, 40（6）: 839-852.
[23]	乔栋,刘勇,田书勇,张锋,冯雪瑾,等. 不同土壤水势对毛白杨苗木生长节律和苗木质量的影响[J]. 北京林业大学学报,2022,44(4):12−23. Qiao D,Liu Y,Tian SY,Zhang F,Feng XJ,et al. Effects of different soil water potentials on seedling growth rhythm and seedling quality of Populus tomentosa[J]. Journal of Beijing Forestry University,2022,44 (4):12−23.
[24]	王苗苗,刘勇,李国雷,彭玉信,刘春和,等. 秋季施肥对毛白杨苗木质量、造林效果和养分回流的影响[J]. 林业科学,2021,57(7):51−60. Wang MM,Liu Y,Li GL,Peng YX,Liu CH,et al. Effects of autumn fertilization on quality,field performance and nutrient resorption of Populus tomentosa seedling[J]. Scientia Silvae Sinicae,2021,57 (7):51−60.
[25]	杨阳,施皓然,及利,杨立学. 指数施肥对紫椴实生苗生长和根系形态的影响[J]. 南京林业大学学报(自然科学版),2020,44(2):91−97. Yang Y,Shi HR,Ji L,Yang LX. Effects of exponential fertilization on growth and root morphology of Tilia amurensis seedlings[J]. Journal of Nanjing Forestry University (Natural Sciences Edition),2020,44 (2):91−97.
[26]	王燕,晏紫依,苏艳,何茜,李吉跃,等. 不同施肥方法对欧洲云杉生长生理和根系形态的影响[J]. 西北林学院学报,2015,30(6):15−21. Wang Y,Yan ZY,Su Y,He Q,Li JY,et al. Effects of different fertilizing methods on growth,physiological characteristics and root morphological traits of Picea abies[J]. Journal of Northwest Forestry University,2015,30 (6):15−21.
[27]	Montagnoli A,Terzaghi M,Di Iorio A,Scippa GS,Chiatante D. Fine-root morphological and growth traits in a Turkey-oak stand in relation to seasonal changes in soil moisture in the Southern Apennines,Italy[J]. Ecol Res,2012,27 (6):1015−1025. doi: 10.1007/s11284-012-0981-1
[28]	Margolis HA,Waring RH. Carbon and nitrogen allocation patterns of Douglas-fir seedlings fertilized with nitrogen in autumn. Ⅰ. Overwinter metabolism[J]. Can J Forest Res,1986,16 (5):897−902. doi: 10.1139/x86-160
[29]	Hong Z,Guo JY,Zhang NN,Yang ZJ,Liu XJ,et al. Polysaccharide,proline,and anti-oxidation enzyme activities of Thailand rosewood (Dalbergia cochinchinensis) seedlings exposed to exponential fertilization[J]. J Forest Res,2022,33 (1):75−87. doi: 10.1007/s11676-021-01300-4
[30]	王睿照,毛沂新,云丽丽,尤文忠,张慧东. 氮添加对蒙古栎叶片碳氮磷化学计量与非结构性碳水化合物的影响[J]. 生态学杂志,2022,41(7):1369−1377. Wang RZ,Mao YX,Yun LL,You WZ,Zhang HD. Effects of nitrogen addition on leaf carbon,nitrogen and phosphorus stoichiometry and nonstructural carbohydrates in Mongolian oak (Quercus mongolica) stands[J]. Chinese Journal of Ecology,2022,41 (7):1369−1377.
[31]	唐新瑶,亢亚超,梁喜献,马道承,王凌晖. 氮磷钾配比施肥对观光木幼苗生理与光合特性的影响[J]. 西北林学院学报,2022,37(4):37−42. Tang XY,Kang YC,Liang XX,Ma DC,Wang LH. Effects of N,P and K proportional fertilization on the physiological and photosynthetic characteristics of Tsoongiodendron odorum seedlings[J]. Journal of Northwest Forestry University,2022,37 (4):37−42.

[1]	Li Lin, Fu Qiang, Yang Tao, Mo Xiaolian, Chen Xiaolong, Zhao Jiehong, Zou Jie. cDNA yeast library construction of Dendrobium officinale Kimura et Migo seeds and screening and analysis of DELLA interacting proteins[J]. Plant Science Journal, 2025, 43(2): 221-229. DOI: 10.11913/PSJ.2095-0837.24215
[2]	Jia Yanru, Jin Yufan, Jiao Yuan, Zhou Yingxu, Shi Yang, Chen Ji, Chen Yani, Huang Yanyan, Huang Jin. A review on the research progress of the Phytocyanin (PC) protein family[J]. Plant Science Journal, 2024, 42(5): 644-653. DOI: 10.11913/PSJ.2095-0837.23290
[3]	Kang Ya-Chao, Liu Ping, Wang Ling-Hui, Mo Zhe, Qin Li-Ting, Teng Wei-Chao. Physiological responses of Erythrophleum fordii seedlings under SNP-AlCl₃ interaction[J]. Plant Science Journal, 2019, 37(4): 521-529. DOI: 10.11913/PSJ.2095-0837.2019.40521
[4]	Liu Ya-Lin, Wu Xiu-Wen, Yan Lei, Du Chen-Qing, Jiang Cun-Cang. Research progress on the effect of boron and calcium on plants and the interaction mechanism in the cell wall[J]. Plant Science Journal, 2018, 36(5): 767-773. DOI: 10.11913/PSJ.2095-0837.2018.50767
[5]	Jin Chun-Mei, Zhou Kun, Zhang Jin-Jin. Interactions of MADS-box transcription factors CsGLO1, CsGLO2 and CsAG in Camellia sinensis flower development[J]. Plant Science Journal, 2017, 35(1): 79-86. DOI: 10.11913/PSJ.2095-0837.2017.10079
[6]	GAO Shuang, LIU Xiao-Chen, DONG Zheng, LIU Mao-Yan, DAI Liang-Ying. Advance of Phyllosphere Microorganisms and Their Interaction with the Outside Environment[J]. Plant Science Journal, 2016, 34(4): 654-661. DOI: 10.11913/PSJ.2095-0837.2016.40654
[7]	OUYANG Mo, TANG Xiao, HUANG Xi, YUAN Hong-Mei. Construction of Yeast Two-hybrid Bait Vector and the Screening of Proteins Interacting with HbICE1 in Hevea brasiliensis[J]. Plant Science Journal, 2016, 34(2): 255-262. DOI: 10.11913/PSJ.2095-0837.2016.20255
[8]	YANG Cheng-Li, LIU De-Li. Progress in the Studies of Sweet Protein Thaumatin[J]. Plant Science Journal, 2001, 19(2): 153-157.
[9]	Sun Delan, Dong Yunzhou. ANALYSES OF ELECTROPHORETIC PATTERN OF AMYLOPLAST PROTEIN AND TOTAL PROTEIN IN PLANT STORE ORGANS[J]. Plant Science Journal, 1999, 17(4): 375-377.
[10]	Sun Guchou. SUBUNITS OF PHYCOCYANIN AND F₆₇₈ PIGMENT-PROTEIN IN ANABAENA CYLINDRICA[J]. Plant Science Journal, 1987, 5(4): 383-390.

Cited By

Cited by

Periodical cited type(1)

冷凇凝，谭延肖，张煜，吴玉森，韩双，陈晓乐. 楸子叶片酵母文库构建及甘油二酯激酶MpDGK7互作蛋白鉴定. 核农学报. 2024(09): 1691-1698 .

Other cited types(0)

Get Citation

PDF

XML

Article views (166) PDF downloads (26) Cited by(1)

Turn off MathJax

Article Contents

Abstract

References

Regulatory effects of fertilization on root morphology and needle physiological characteristics of Pinus armandii Franch. container seedlings