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JIN Tong-Xia, DONG Wen-Jing, GUO Meng, DAI Ke-Yan, YANG Cheng, XU Ting-Ting, MA Jian-Min. Effects of Carbon,Nitrogen,and Phosphorus Concentration in Eutrophic Wastewater on Total Lipids and Total Hydrocarbons of Botryococcus braunii[J]. Plant Science Journal, 2012, (3): 293-298. DOI: 10.3724/SP.J.1142.2012.30293
Citation: JIN Tong-Xia, DONG Wen-Jing, GUO Meng, DAI Ke-Yan, YANG Cheng, XU Ting-Ting, MA Jian-Min. Effects of Carbon,Nitrogen,and Phosphorus Concentration in Eutrophic Wastewater on Total Lipids and Total Hydrocarbons of Botryococcus braunii[J]. Plant Science Journal, 2012, (3): 293-298. DOI: 10.3724/SP.J.1142.2012.30293

Effects of Carbon,Nitrogen,and Phosphorus Concentration in Eutrophic Wastewater on Total Lipids and Total Hydrocarbons of Botryococcus braunii

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  • Received Date: December 21, 2011
  • Revised Date: March 05, 2012
  • Published Date: June 29, 2012
  • Eutrophication wastewater from twice filtered fishponds was used as the culture medium,into which carbon,nitrogen,and phosphorus were added.The influences of different concentrations of inorganic carbon,total nitrogen,and total phosphorus of sewage on biomass,li-pid content,and hydrocarbon content of Botryococcus braunii were studied.The following results were reported: (1) Using NaHCO3 as the carbon source,the biomass and lipid content of B. braunii were the highest at the inorganic carbon concentration of 5-10 mg/L,while the highest hydrocarbon content was at the inorganic carbon concentration of 15 mg/L.(2) Using KNO3 as the nitrogen source,the biomass of B. braunii was the highest at the total nitrogen concentration of 15 mg/L; the highest lipid content was at the total nitrogen concentration of 2 mg/L; the highest hydrocarbon content was at the total nitrogen concentration of 20 mg/L.(3) Using KH2PO4 as the phosphorus source,the biomass of B. braunii was the highest at a total phosphate concentration of 2 mg/L while the highest lipid content and the highest hydrocarbon content were at a total phosphorus concentration of 1.5 mg/L.
  • [1]
    梅洪,张成武,殷大聪,耿亚红,欧阳峥嵘,李夜光. 利用微藻生产可再生能源研究概况[J]. 武汉植物学研究, 2008, 26(6): 650-660.
    [2]
    金相灿,屠清瑛.湖泊富营养化调查规范[M].北京: 中国环境科学出版社, 1990: 10-11.
    [3]
    王军,杨素玲,丛威,蔡昭铃.营养条件对产烃葡萄藻生长的影响[J].过程工程学报, 2003, 3(2): 141-145.
    [4]
    Banerjee A,Sharma R,Chisti Y,Banerjee U C.Botryococcus braunii: a renewable source of hydrocarbons and other chemicals [J].Crit Rev Biotechnol, 2002, 22(3): 245-279.
    [5]
    马梅,王朋云.布朗葡萄藻培养方面的研究概况[J].现代农业科技, 2008(15): 35-38.
    [6]
    Casadevall E,Dif D,Largeau C.Studies on batch and continuous cultures of Botryococcus braunii[J].Biotechnol Bioeng, 1985, 27(3): 286-295.
    [7]
    王修垣.高产烃的丛粒藻研究概况[J].微生物学报, 1997, 37(5): 405-409.
    [8]
    Bligh E G,Dyer W J.A rapid method of total lipid extraction and purification [J].Can J Biochem Phycol, 1959,37(8): 911-917.
    [9]
    Chen F,John M R.Effect of C/N Ratio and aeration on the fatty acid composition of heterotrophic Chlorella sorokiniana[J].J Appl Phycol, 1991, 3(3): 203-209.
    [10]
    Sawayama S,Minowa T,Dotc Y.Growth of the hydrocarbon rich microalga Botryococcus braunii in secondarily treated sewage [J].Appl Microbiol Biotechnol, 1992, 38(2): 135-138.
    [11]
    Sheehan J,Dunnahay T,Benemann J,Roessler P.A Look Back at the U.S.Department of Energy’s Aquatic Species Program: Biodiesel From Algae.The National Renewable Energy Laboratory of the US Department of Energy, 1998: 328.
    [12]
    Wolf F R,Nanomura A M,Bassh J A.Growth and branched hydrocarbon production a strain of Botryococcus braunii[J]. J Phycol, 1985, (21): 388-396.
    [13]
    Liliana R,Graziella C Z,Niccolò B,Giulia P,Natascia B,Gimena B,Mario R T.Microalgae for oil: strain selection,induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor [J]. Biotechnol Bioeng, 2009, 102(1): 100-102.
    [14]
    Singh Y,Kumar H D.Lipid and hydrocarbon production by Botryococcus spp.under nitrogen limitation and an aerobiosis [J]. World J Microbiol Biotechno, 1992, 8: 121-124.
    [15]
    孙凯峰,胡章喜,段舜山. N、P营养盐胁迫对两株布朗葡萄藻生长的影响[J]. 水生生物学报, 2010, 34(3): 517-524.
    [16]
    吴夏芫,李环,韦萍.能源微藻——葡萄藻培养的研究进展[J].安徽农业科学, 2008, 36(20): 8755-8756,8760.
    [17]
    华汝成.单细胞藻类的培养与利用[M].北京: 农业出版社, 1983: 135-137.
    [18]
    Kojima E,Zhang K.Growth and hydrocarbon production by microalga Botryococcus braunii in bubble column photobioreactor [J].J Bio SCI Bioeng, 1999(87): 811-815.
    [19]
    Órpez R,Martínez M E,Gassan H,Yousfi F E,Jbari N,Sónchez S.Growth of the microalga Botryococcus braunii in secondarily treated sewage [J].Desalination, 2009, 246: 625-630.
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