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QIN Min, WU Ping-Ping, YAO Ming-Jing. Plant Regeneration from Different Explants of Perilla frutescens L.Britton[J]. Plant Science Journal, 2013, 31(2): 151-157. DOI: 10.3724/SP.J.1142.2013.20151
Citation: QIN Min, WU Ping-Ping, YAO Ming-Jing. Plant Regeneration from Different Explants of Perilla frutescens L.Britton[J]. Plant Science Journal, 2013, 31(2): 151-157. DOI: 10.3724/SP.J.1142.2013.20151
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Plant Regeneration from Different Explants of Perilla frutescens L.Britton

  • Institute of Medicinal Plant, College of Plant Science & Technology of Huazhong Agricultural University, Wuhan 430070, China

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  • Received Date: May 15, 2012
  • Revised Date: September 17, 2012
  • Published Date: April 29, 2013
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    • Abstract
  • Tissue culture and regeneration of the medicinal plant, Perilla frutescens, were investigated from different explants with different concentrations and combinations of hormones, and an efficient regeneration system was established. Explants showed high to low regeneration in the order terminal bud, lamina, cotyledon and hypocotyl. The regeneration rate of terminal bud was above 85% with or without hormone, and the addition of appropriate hormone promoted the proliferation rate. The regeneration rate of lamina was 70% on average with 0.05-0.1 mg/L TDZ. Optimal hormone combinations of cotyledon and hypocotyl were MS+KT 1.0 mg/L + IAA 1.0 mg/L and MS + 6-BA 1.0 mg/L + IAA 1.0 mg/L, with regeneration rates of 22.22% and 14.61%, respectively. The best rooting medium was 1/2 MS medium without hormone, leading to rooting rate of 86.67% and root length of 7.36 cm, on average. Explants from lamina could form leaf clusters, which induced adventitious buds repeatedly and proliferated quickly.
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    • References (14)
  • [1]
    方嘉禾, 常汝镇. 中国作物及其野生近缘植物: 经济作物卷[M]. 北京: [JP2]中国农业出版社, 2007: 222-223.
    [2]
    蒲海燕, 李影球, 李梅. 紫苏的功能性成分及其产品开发[J]. 中国食品添加剂, 2009(2): 133-137.
    [3]
    黄丹, 刘达玉. 紫苏提取物抑菌特性研究[J]. 食品工业, 2007(3): 11-13.
    [4]
    Kim J A, Choi H J, Park S K, Kim D U. Callus induction and plant regeneration from leaf segments and cotyledonary explants of Perilla frutescens[J]. Korean J Plant Tissue Cult, 1993, 20(1): 47-50.
    [5]
    Kim K H, Lee Y H, Kim D, Park Y H, Lee J Y, Hwang Y S, Kim Y H. Agrobacterium-mediated genetic transformation of Perilla frutescens[J]. Plant Cell Rep, 2004, 23(6): 386-390.
    [6]
    Hou S W, Jia J F. In vitro regeneration of Perilla frutescens from hypocotyl and cotyledon explants[J]. Biol Plant, 2005, 49(1): 129-132.
    [7]
    Zhang T, Wang X Y, Cao Z Y. Plant regeneration in vitro directly from cotyledon and hypocotyl explants of Perilla frutescens and their morphological aspects[J]. Biol Plant, 2005, 49(3): 423-426.
    [8]
    牛炳韬. 紫苏离体再生体系的建立及体细胞无性系变异分析 [D]. 兰州:兰州大学, 2005.
    [9]
    黄海泉,张蕾,黄美娟. 紫苏组织培养及植株再生[J]. 亚热带植物科学, 2007, 36(4): 56.
    [10]
    于淑玲,石晓云. 紫苏组织培养体系的研究[J]. 邢台学院学报, 2008, 23(2): 118-125.
    [11]
    周川云, 戴向辰,胡彦. 紫苏不同外植体组培灭菌条件研究[J]. 广西农业科学, 2009, 40(4): 340-343.
    [12]
    刘培培. 紫苏离体再生体系的建立及其遗传转化初步研究[D].太原: 中北大学, 2011.
    [13]
    杨念, 曾宋君, 吴坤林, 陈之林, 段俊. 广藿香愈伤组织的诱导和增殖[J]. 福建林业科技, 2006, 33(3): 68-72.
    [14]
    Hossain H M M T, Kim Y H, Lee Y S. The apical bud as a novel explant for high-frequency in vitro plantlet regeneration of Perilla frutescens L. Britton[J]. Plant Biotechnol Rep, 2010, 4(3):229-235.
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