Recent Advances in Fluorescence in situ Hybridization

HE Shi-Bin; CHAI Lian-Qin; TAN Jun-Jun; SHEN Yao; ZHOU Ping; MA Yun-Feng; LI Li-Jia

doi:10.3724/SP.J.1142.2014.20199

Plant Science Journal > 2014 > 32(2): 199-204. > DOI: 10.3724/SP.J.1142.2014.20199 CSTR: 32231.14.SP.J.1142.2014.20199

HE Shi-Bin, CHAI Lian-Qin, TAN Jun-Jun, SHEN Yao, ZHOU Ping, MA Yun-Feng, LI Li-Jia. Recent Advances in Fluorescence in situ Hybridization[J]. Plant Science Journal, 2014, 32(2): 199-204. DOI: 10.3724/SP.J.1142.2014.20199

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Recent Advances in Fluorescence in situ Hybridization

1. College of Life Sciences, Henan University, Kaifeng, Henan 475004, China;
2. College of Life Sciences, Wuhan University, Wuhan 430072, China

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Received Date: January 09, 2014
Revised Date: March 04, 2014
Available Online: November 01, 2022
Published Date: April 29, 2014

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Abstract

Abstract

Fluorescence in situ hybridization(FISH) is an effective tool for mapping specific DNA sequences on chromosomes, interphase nuclei, and DNA fibers. In recent years, rapid development of FISH has been made by improving resolution and sensitivity and by combining immunostaining, quantum dots and microfluidic chips, and other physical and chemical technologies. We reviewed the basic principles and research advances in FISH, especially the most recent technologies of immuno-FISH, quantum dot-FISH, and FISH on microchip. Their rapid, sensitive, dynamic, and diverse characteristics were also discussed. With its continued improvement and development, FISH will play an increasingly important role in cytogenetics, epigenetics, molecular biology and other research areas.
- Fluorescence in situ hybridization(FISH),
- Immunostaining,
- Quantum dot(QD),
- Microfluidic chip

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References

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