Analysis on Volatile Components of Caryophylli Flos from Different Habitats

We analyzed the volatile chemical components of Caryophylli Flos from different habitats using headspace solid phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and roughly quantified by peak area normalization. In total, 72 peaks were identified from 13 batches of Caryophylli Flos. Among the volatile chemical components, Eugenol and (-)-α-Selinene accounted for the first two main components, with third-ranked components of the four habitats being cis-α-bisabolene (Madagascar Tamatave, average 3.75%), Ocimene (Guangdong Province, China, average 4.21%), Santolinatriene (Guangxi Province, China, average 3.74%), Humulene (Indonesia Java, average 3.60%). These results suggest that the volatile components of Caryophylli Flos differed in different regions, but showed similarities within the same region. Using principal component analysis (PCA) and cluster analysis, results showed that the four habitat samples of Caryophylli Flos were not only easily distinguished, but also reflected a genetic relationship between them. HS-SPME-GC-MS combined with stoichiometry (PCA and cluster analysis) was able to distinguish the four origins of Caryophylli Flos. This study provided a new approach for the comparison and quality assessment of Caryophylli Flos from different habitats.