Dynamic Changes of Methylcytosine in Polarized Cell Growth and Their Response to GABA Signals

CUI Guo-Peng; WANG Sheng-Jie; QIN Rui; LIU Hong; GONG Han-Yu; LI Gang; YU Guang-Hui

doi:10.11913/PSJ.2095-0837.2015.40521

Plant Science Journal > 2015 > 33(4): 521-527. > DOI: 10.11913/PSJ.2095-0837.2015.40521 CSTR: 32231.14.PSJ.2095-0837.2015.40521

CUI Guo-Peng, WANG Sheng-Jie, QIN Rui, LIU Hong, GONG Han-Yu, LI Gang, YU Guang-Hui. Dynamic Changes of Methylcytosine in Polarized Cell Growth and Their Response to GABA Signals[J]. Plant Science Journal, 2015, 33(4): 521-527. DOI: 10.11913/PSJ.2095-0837.2015.40521

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Dynamic Changes of Methylcytosine in Polarized Cell Growth and Their Response to GABA Signals

Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Engineering Research Centre for the Protection and Utilization of Bioresource in Ethnic Area of Southern China, College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China

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Received Date: January 18, 2015
Published Date: August 27, 2015

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Abstract

Abstract

DNA cytosine methylation (5mC) plays an important role in plant development. A variety of environmental factors, such as cellular endogenous and exogenous stress factors, can trigger changes in DNA methylation. To explore the possible mechanism regulated by γ-aminobutyric acid (GABA) in plant development, we analyzed the content of 5mC and its response to GABA signals in the polarized growth of tobacco pollen tubes and Arabidopsis roots. Results showed that GABA (1.0 mmol/L) significantly promoted the growth of tobacco pollen tubes and Arabidopsis roots, significantly decreased the content of 5mC in the genomic DNA of tobacco pollen tubes and Arabidopsis roots, and increased the content of 5-hydroxyl cytosine (5hmC). 5hmC is an important intermediate of 5mC active demethylation via its oxidative metabolic pathway. Our results demonstrated that GABA, as an important exogenous signal, could regulate dynamic changes in DNA methylation.
- γ-Aminobutyric acid(GABA),
- Methylated DNA,
- 5-Methylcytosine(5mC),
- 5-Hydroxyl methylcytosine(5hmC)

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References

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