Preparation and Structural Characterization of wPNP-a1 from <i>Pholiota nameko</i>

GUO Guo-Yun; SUN Run-Guang; GUO Qi; ZHANG Xiao-Fei; DOU Pei-Juan

doi:10.3724/SP.J.1142.2012.40423

Plant Science Journal > 2012 > 30(4): 423-430. > DOI: 10.3724/SP.J.1142.2012.40423 CSTR: 32231.14.SP.J.1142.2012.40423

GUO Guo-Yun, SUN Run-Guang, GUO Qi, ZHANG Xiao-Fei, DOU Pei-Juan. Preparation and Structural Characterization of wPNP-a1 from Pholiota nameko[J]. Plant Science Journal, 2012, 30(4): 423-430. DOI: 10.3724/SP.J.1142.2012.40423

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Preparation and Structural Characterization of wPNP-a1 from Pholiota nameko

Laboratory of Biophysics and Biomedical Technology, College of Physics and Information Technology, Shaanxi Normal University, Xi’an 710062, China

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Received Date: November 02, 2011
Revised Date: February 18, 2012
Published Date: August 29, 2012

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Abstract

Abstract

Water-soluble crude polysaccharide (wPNP) was extracted from cultured mycelium of the fungus Pholiota nameko by hot water extraction.The extraction rates of polysaccharide were 2.463%.The content of total polysaccharide was determined by the phenol-sulphuric acid method,and the value was 64.98%.The crude polysaccharides were purified by CTAB and Sephadex-150 in sequence,with the obtained one designated as wPNP-a1.The structure of wPNP-a1 was analysed by HPLC,FTIR,GC,NMR,ESEM,and AFM.The results showed that the average molecular weight of wPNP-a1 was 419310 Da.It had the general characteristics of polysaccharide absorption peaks,β-glycoside linkage,and no acetyl.The GC analysis indicated wPNP-a1 was composed of xylose,mannose,galactose,and glucose in molar ratios of 3.91∶2.77∶1.91∶1.The repelling forces between the molecules were stronger than the attractive forces. The wPNP-a1 formed a linear chain on the surface of mica,the width was 23 nm,and the height was 0.7-0.8 nm.
- Pholiota nameko,
- Pholiota nameko polysaccharides,
- Hot water extraction,
- Structure characterization

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