Influence of Salicylic Acid and Methyl Jasmonate on Leaf Microstructure,Leaf Photosynthesis,and Non-structure Sugar Accumulation of <i>Salvia miltiorrhiza</i> Bunge Seedlings

WANG Chun-Li; LIANG Zong-Suo; LI Dian-Rong; YANG Jian-Li

doi:10.3724/SP.J.1142.2012.50501

Plant Science Journal > 2012 > 30(5): 501-510. > DOI: 10.3724/SP.J.1142.2012.50501

WANG Chun-Li, LIANG Zong-Suo, LI Dian-Rong, YANG Jian-Li. Influence of Salicylic Acid and Methyl Jasmonate on Leaf Microstructure,Leaf Photosynthesis,and Non-structure Sugar Accumulation of Salvia miltiorrhiza Bunge Seedlings[J]. Plant Science Journal, 2012, 30(5): 501-510. DOI: 10.3724/SP.J.1142.2012.50501

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Influence of Salicylic Acid and Methyl Jasmonate on Leaf Microstructure,Leaf Photosynthesis,and Non-structure Sugar Accumulation of Salvia miltiorrhiza Bunge Seedlings

1.
College of Life Sciences,Northwest Agriculture & Forestry University, Yangling, Shaanxi 712100, China
2. Hybrid Rapeseed Research Center of Shaanxi Province, Dali, Shaanxi 715105, China

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Received Date: March 06, 2012
Revised Date: April 18, 2012
Published Date: October 29, 2012

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Abstract

Abstract

We investigated the effects of salicylic acid (SA) and methyl jasmonate (MeJA) foliar applications on photosynthesis and accumulation of non-structure sugar of Salvia miltiorrhiza Bunge seedlings.Results showed that SA treatment was favorable in developing leaf microstructure and increased leaf stomata density.Intercellular space in the mesophyll tissue,size of mesophyll cell,and number of chloroplast per mesophyll cell were also reduced,but the size of chloroplast and number of granum in the chloroplast were markedly increased.Correspondingly,contents of chlorophyll a and b in the leaves,net photosynthetic rate of the leaves,and contents of sucrose and soluble sugar in shoots of the SA-treated seedling were together significantly increased following decreased activity of acid invertase in both shoots and roots.The MeJA application to the seedlings resulted in contrary effects in decreasing density of leaf stomata,incurring a number of abnormal stomata,decreasing layer of palisade cell and increasing intercellular space in mesophyll tissue,while number and size of chloroplast was notably reduced and granum in the chloroplast was destroyed.The MeJA treatment also decreased contents of chlorophyll a and carotenoid in leaves but significantly increased activity of acid invertase in both shoots and roots of the seedlings;as a consequence leaf net photosynthetic rate,contents of sucrose and total amount of soluble sugar in roots of the seedlings were decreased. In conclusion,the SA application to S.miltiorrhiza seedling accelerated sucrose accumulation by promoting the development of leaf microstructure to accelerate photosynthesis and inhibiting sucrose hydrolysis,while MeJA application to the seedlings decreased sucrose accumulation by damaging leaf microstructure to impair photosynthesis and accelerating sucrose hydrolysis.
- Salvia miltiorrhiza Bunge seedlings,
- Salicylic acid,
- Methyl jasmonate,
- Microstructure,
- Sucrose accumulation

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References (52)

References

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Influence of Salicylic Acid and Methyl Jasmonate on Leaf Microstructure,Leaf Photosynthesis,and Non-structure Sugar Accumulation of Salvia miltiorrhiza Bunge Seedlings

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