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WANG Yan, CHEN Xi, YANG Zhong-Min, LIU Deng-Hui. Cloning of Pathogen-Related Protein Gene(SfPR-1) from Salsola ferganica and Its Expression Analysis under Salt Stress[J]. Plant Science Journal, 2013, 31(2): 164-170. DOI: 10.3724/SP.J.1142.2013.20164
Citation: WANG Yan, CHEN Xi, YANG Zhong-Min, LIU Deng-Hui. Cloning of Pathogen-Related Protein Gene(SfPR-1) from Salsola ferganica and Its Expression Analysis under Salt Stress[J]. Plant Science Journal, 2013, 31(2): 164-170. DOI: 10.3724/SP.J.1142.2013.20164
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Cloning of Pathogen-Related Protein Gene(SfPR-1) from Salsola ferganica and Its Expression Analysis under Salt Stress

  • Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China

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  • Received Date: June 25, 2012
  • Revised Date: November 16, 2012
  • Published Date: April 29, 2013
    Graphical Abstract
    • Abstract
  • A cDNA fragment was isolated from Salsola ferganica by suppression subtractive hybridization and its full-length cDNA with 817 bp was cloned by SMARTTM RACE, and was named SfPR-1 gene (GenBank accession number: JQ670917). The SfPR-1 gene consisted of a 501 bp open reading frame encoding 166 amino acids with molecular weight of 18.01 kDa and an isoelectric point of 9.37, a 65 bp 5'-UTR and 251 bp 3'-UTR. The deduced amino acid sequence of SfPR-1 showed high identity of 73.6%, 57.8%, 55.5% and 53.9% to those of pathogen-related protein 1 from Beta vulgaris, Arabidopsis thaliana, Nicotiana tabacum and Zea mays, respectively, and had a conserved six-cysteine motif. Reverse transcriptase PCR and Real-time PCR methods were used to investigate the expression profile of the SfPR-1 gene under salt stress. SfPR-1 showed up-regulated expression patterns under salt treatment. Based on our results, we concluded that the SfPR-1 gene might be involved in salt response and an important component for the salt tolerant pathway in Salsola ferganica.
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    • References (31)
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