Screening of Drought- and Salinity-responsive EST-SSR Markers in Medicago ruthenica Trautv.

To provide genetic markers for the study of ecological adaptability to responsive drought- and salinity-stress for Medicago ruthenica Trautv.,we selected eight pairs of good polymorphism and high stability primers from 70 pairs of drought-and salinity-responsive EST-SSR markers published for chickpea.DNA sequences were amplified with eight pairs of SSR primers by SSR-PCR technique.One hundred and eleven alleles were detected,on average,13.88 alleles were detected per site.The mean values of observed heterozygosity and expected heterozygosity were 0.497 and 0.687,respectively.Polymorphism information content value ranged from 0.313 to 0.883, and the mean value was 0.649.These results indicate that the eight EST-SSR markers can be used to estimate genetic diversity of M.ruthenica,and genetic diversity was at a high level.Polymorphism EST-SSR markers can be used to investigate ecological adaptive evolution.The results contribute to revealing the genetic variation of drought- and salt-tolerance genotypes and understanding the distribution pattern and ecological adaptation mechanism of M.ruthenica populations.