Advance Search
LIU Jie, HU Die, CHU Hai-Jia, YAN Juan, LI Jian-Qiang. Screening of Drought- and Salinity-responsive EST-SSR Markers in Medicago ruthenica Trautv.[J]. Plant Science Journal, 2013, 31(5): 493-499. DOI: 10.3724/SP.J.1142.2013.50493
Citation: LIU Jie, HU Die, CHU Hai-Jia, YAN Juan, LI Jian-Qiang. Screening of Drought- and Salinity-responsive EST-SSR Markers in Medicago ruthenica Trautv.[J]. Plant Science Journal, 2013, 31(5): 493-499. DOI: 10.3724/SP.J.1142.2013.50493
shu

Screening of Drought- and Salinity-responsive EST-SSR Markers in Medicago ruthenica Trautv.

  • 1.

    Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China

  • 2. University of Chinese Academy of Science, Beijing 100049, China

More Information
  • Received Date: March 10, 2013
  • Revised Date: August 12, 2013
  • Published Date: October 29, 2013
    Graphical Abstract
    • Abstract
  • To provide genetic markers for the study of ecological adaptability to responsive drought- and salinity-stress for Medicago ruthenica Trautv.,we selected eight pairs of good polymorphism and high stability primers from 70 pairs of drought-and salinity-responsive EST-SSR markers published for chickpea.DNA sequences were amplified with eight pairs of SSR primers by SSR-PCR technique.One hundred and eleven alleles were detected,on average,13.88 alleles were detected per site.The mean values of observed heterozygosity and expected heterozygosity were 0.497 and 0.687,respectively.Polymorphism information content value ranged from 0.313 to 0.883, and the mean value was 0.649.These results indicate that the eight EST-SSR markers can be used to estimate genetic diversity of M.ruthenica,and genetic diversity was at a high level.Polymorphism EST-SSR markers can be used to investigate ecological adaptive evolution.The results contribute to revealing the genetic variation of drought- and salt-tolerance genotypes and understanding the distribution pattern and ecological adaptation mechanism of M.ruthenica populations.
    • FullText(HTML)
    • References (27)
  • [1]
    汤章城.植物对水分胁迫的反应和适应性:Ⅰ. 抗逆性的一般概念和植物的抗涝性[J]. 植物生理学通讯, 1983(3): 24-29.
    [2]
    王士谦.玉米抗旱性研究进展[J].河北科技师范学院学报, 2005, 19(3): 76-80.
    [3]
    俞仁培,陈德明.我国盐渍土资源及其开发利用[J].土壤通报, 1999, 30(4): 158-159.
    [4]
    韦直,黄以之.中国植物志:第42卷,第2分册[M].北京: 科学出版社, 1998: 318-320.
    [5]
    Balabaev G A.Yellow lucernes of Siberia,Medicago ruthenica (L.) Lebd. and M.Platycarpos (L.) Lebd.[J].Bull App Bot Genet Plant Breed Service, 1934, 7: 113-123.
    [6]
    Campbell T A,Bao G,Xia Z L.Agronomic evaluation of Medicago ruthenica collected in Inner Mongolia[J].Crop Sci, 1997, 37: 599-604.
    [7]
    Varshney R K,Graner A,Sorrells M E.Genic microsatellite markers in plants: features and applications[J].Trends Biotechnol, 2005, 23(1): 48-55.
    [8]
    Kong Q,Xiang C,Yu Z.Development of EST SSRs in Cucumis sativus from sequence database[J].Mol Ecol Resour, 2006, 6(4): 1234-1236.
    [9]
    Eujay I,Sledge M K,Wang L,May G D,Chekhovskiy K,Zwonitzer J C,Mian M A R.Medicago truncatula EST-SSR reveal crosspecies genetic markers for Medicago spp.[J].Theor Appl Ge-net, 2004, 108(3): 414-422.
    [10]
    Gupta P K,Rustgis S,Sharma S,Singh R,Kumar N,Balyan H S.Transferable EST-SSR mar-kers for the study of polymorphism and genetic diversity in bread wheat[J].Mol Genet Genomics, 2003, 270: 315-323.
    [11]
    Nicot N,Chiquet V,Gandon B,Amilhat L,Legeai F,Leroy P,Bernard M,Sourdille P.Study of simply sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs)[J].Theor Appl Genet, 2004, 109: 800-805.
    [12]
    Temnykh S,Park W D,Ayree N,Cartinhour S,Hauck N,Lipovich L,Cho Y G,Ishii T,McCouch S R.Mapping and genome organization of microsatellite sequence in rice (Oryza sativa L.)[J].Theor Appl Genet, 2000, 100: 697-712.
    [13]
    Liang X Q,Chen X P,Hong Y B,Liu H Y,Zhou G Y,Li S X,Guo B Z.Utility of EST-derived SSR in cultivated peanut (Arachis hypogaea L.) and Arachis wild species[J].BMC Plant Biol, 2009, 9: 35.
    [14]
    Scott K D,Eggler P,Seaton G,Rossetto M,Ablett E M,Lee L S,Henry R J. Analysis of SSRs derived from grape ESTs[J]. Theor Appl Genet, 2000, 100: 723-726.
    [15]
    Han Z G,Guo W Z,Song X L,Zhang T Z. Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton[J]. Mol Genet Genomics, 2004, 272: 308-327.
    [16]
    Bruno S,Torben A,Ursula F. Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)[J]. Mol Breed, 2008, 21: 533-548.
    [17]
    Varshney R K,Hiremath P J,Lekha P,Kashiwagi J,Balaji J,Deokar A A,Vadez V,Xiao Y L,Srinivasan R,Gaur P M,Siddique K H M,Town C D,Hoisington D A. A comprehensive resource of drought-and salinity-responsive ESTs for gene discovery and marker development in chickpea (Cicer arietinum L.)[J].BMC Genomics, 2009, 10: 523.
    [18]
    Sanguinetti C J,Dias N E,Simpson A J. Rapid silver staining and recovery of PCR products separated on polyaerylamide gels[J].Biotechniques, 1994, 17: 915-919.
    [19]
    Kalinowski S T, Taper M L, Marshall T C. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment[J]. Mol Ecol, 2007, 16: 1099-1106.
    [20]
    Raymond M,Rousset F. GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism[J].J Hered, 1995, 86(3): 248-249.
    [21]
    Botsein D,White R L,Skolnick M C,Davis R W. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].Am J Hum Genet, 1980, 32(3): 314-331.
    [22]
    Saha M C,Mian M A R,Eujayl I,Zwonitzer J C,Wang L J,May G D.Tall fescue EST-SSR mar-kers with transferability across several grass species[J]. Theor Appl Genet, 2004, 109: 783-791.
    [23]
    Gao L F,Tang J,Li H,Jia J Z.Analysis of microsatellites in major crops assessed by computational and experimental approaches[J].Mol Breed, 2003, 12: 245-261.
    [24]
    Gupta P K,Varsheney R K.The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat[J]. Euphytica, 2000, 113: 163-185.
    [25]
    李鸿雁,米福贵,宁红梅,师文贵,赵成贵.扁蓿豆遗传多样性的SSR分析[J].中国草地学报, 2008, 30(2): 34-38.
    [26]
    常玮,赵雪,李侠,邱波,韩英鹏,縢伟丽,李文滨.大豆EST-SSR标记开发及与Genomic SSR的比较研究[J].中国油料作物学报, 2009, 31(2): 149-156.
    [27]
    Frankham R,Ballou J D,Briscoe D A.Introduction to Conservation Genetics[M].Cambridge: Cambridge University Press, 2002: 29-62.
    • Related Articles

  • Related Articles

    [1]Lou Yong-Feng, Gan Ran, Zhu Cheng-Lei, Xiao Fu-Ming, Gao Zhi-Min. Development and application of EST-SSR markers based on transcriptome of Phyllostachys vivax McClure f. aureocaulis N. X. Ma[J]. Plant Science Journal, 2022, 40(3): 355-364. DOI: 10.11913/PSJ.2095-0837.2022.30355
    [2]Liu Fen-Fen, Xu Gang-Biao, Li Tian-Tian, Luo Chang-Sha. Characterization and development of SSR markers from transcriptome sequence of Bretschneidera sinensis Hemsl.[J]. Plant Science Journal, 2021, 39(3): 288-296. DOI: 10.11913/PSJ.2095-0837.2021.30288
    [3]Wagutu Godfrey Kinyori, Njeri Henry Kariuki, Fan Xiang-Rong, Chen Yuan-Yuan. Development and transferability analysis of SSR primers in wild rice Zizania latifolia (Poaceae)[J]. Plant Science Journal, 2020, 38(1): 105-111. DOI: 10.11913/PSJ.2095-0837.2020.10105
    [4]Li Rui-Yuan, Shi Tao-Xiong, Chen Qi-Jiao, Pan Fan, Chen Qing-Fu. Construction of EST-SSR fingerprinting and analysis of genetic diversity of thirty-five registered tartary buckwheat cultivars (Fagopyrum tataricum) in China[J]. Plant Science Journal, 2017, 35(2): 267-275. DOI: 10.11913/PSJ.2095-0837.2017.20267
    [5]GUO Hong-Yuan, JIA Ju-Qing, ZHANG Xian-Hong, LA Shi-Xiao, YANG Wu-De. Development and Application of EST-SSR Markers in Oat[J]. Plant Science Journal, 2014, 32(3): 240-250. DOI: 10.3724/SP.J.1142.2014.30240
    [6]CHENG Xue-Ni, WANG Ying, PANG Yu-Hui, CHEN Xin-Hong, WU Jun, ZHAO Ji-Xin. Primer Transferability Analysis on SSR and EST-SSR Markers from Triticum aestivum to Bromus inermis[J]. Plant Science Journal, 2014, 32(1): 27-33. DOI: 10.3724/SP.J.1142.2014.10027
    [7]CHEN Jin-Jin, PENG Jun-Hua. Transferability of Wheat(Triticum aestivum)EST-derived SSR Markers to Several Potential Energy Plants in Gramineae[J]. Plant Science Journal, 2011, 29(6): 696-703.
    [8]LONG Song-Hua, LI Xiang, DENG Xin, WANG Yu-Fu, WANG Jing, HE Dong-Feng, CHEN Xin-Bo. Flax (Linum sitatissimum L.) EST-SSR Information Analysis and Marker Development[J]. Plant Science Journal, 2010, 28(5): 634-638.
    [9]LI Yan, JIANG Yu-Mei, LU Shun-Bao, PENG Jiu-Sheng, ZHU Du. Optimization of Experiment Conditions and Primer Screening with ISSR Markers for Chimonanthus grammatus[J]. Plant Science Journal, 2008, 26(3): 245-250.
    [10]ZHANG Jun-E, LI Fen, SUN Meng-Xiang. Analysis of EST-SSR Loci in Tobacco (Nicotiana tabacum L.)[J]. Plant Science Journal, 2007, 25(5): 427-431.

Catalog

    Get Citation
    PDF
    XML
    Article views (1387) PDF downloads (1704) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return