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TANG Shao-Qing, DU Lin-Fang, WANG Yan. AFLP Analysis of Ser. Chrysantha Chang (Camellia, Sect. Chrysantha)[J]. Plant Science Journal, 2004, 22(1): 44-48.
Citation: TANG Shao-Qing, DU Lin-Fang, WANG Yan. AFLP Analysis of Ser. Chrysantha Chang (Camellia, Sect. Chrysantha)[J]. Plant Science Journal, 2004, 22(1): 44-48.
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AFLP Analysis of Ser. Chrysantha Chang (Camellia, Sect. Chrysantha)

  • 1.

    Biology Department, Guangxi Normal University, Guilin 541005, China

  • 2.

    College of Life Sciences, Sichuan University, Chengdu 610064, China

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  • Received Date: May 09, 2003
  • Revised Date: July 14, 2003
  • Published Date: February 25, 2004
    Graphical Abstract
    • Abstract
  • Amplified fragment length polymorphism (AFLP) was assessed in 35 accessions of Ser. Chrysantha Chang (Camellia, Sect. Chrysantha) collected from China, including 14 species and 1 variety following Flora of China and other 7 species being controversial in taxonomy. Four primer combinations E-ACG/M-CAG,E-ACG/M-CTG,E-AGG/M-CTG and E-AGG/M-CAT were used for selective amplification, from which Eco RⅠ primers were fluorescent dye-labeled. A total of 298 fragments were detected, in which 278 are polymorphic. We analyzed the AFLP data using Nei & Li similarity coefficient and UPGMA Cluster method. The result suggested that (1) C. huana was a distinctive species; (2) C. longgangensis, C. longruiensis, C. ptilosperma, and C. grandis should be merged into C. flavida .; (3) C. chrysanthoides, C. micrantha, C. xiashiensis and C. parvipetala are closely related and probably conspecific.
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