Isolation and Identification of Metal Resistance Green Alga

A total of 62 green alga strains were isolated from the soils of Nanshan Mountain,Xinjiang.Used the blot method to characterize these metal resistance,the results indicated that XJU3、XJU28 and XJU36 have resistance to 01 mmol•L-1Co2+;XJU28 has resistance to 1 mmol•L-1Zn2+ and Fe3+，XJU36 has resistance to 005 mmol•L-1Cu2+.Taxonomic evaluation of the three strains were investigated based on the morphology and the internal transcribed spacer (ITS) regions(including the 58S).Based on morphological characteristics,the three strains were likely to Chlamydomonas.Phylogenetic reconstruction with the Neighborjoining (NJ) method using sequences of ITS(including the 58S) indicated that XJU3 and XJU28 are closed to Chlamydomonas zebra.XJU36 is closed to Chlamydomonas petasua.