Isolation of High Molecular Weight Glutenin Subunit Gene 1Dy12^* from Yunnan Wheat(Triticum aestivum subsp.yunnanense King)

A novel y-type high molecular weight glutenin subunit(HMW-GS) possessing a slightly faster mobility than that of subunit 1Dy12 was identified through SDS-PAGE analysis,designated 1Dy12^* in Yunnan wheat(Triticum aestivum subsp.yunnanense King).The coding region of HMW-GS gene 1Dy12^* was isolated and sequenced with Glu-Dy locus specific primers.The complete open reading frame(ORF) of 1Dy12^* was 1980 bp,encoding 658 amino acids.Comparison of amino acid sequences showed there were 3 amino acid differences between subunits 1Dy12^* and 1Dy12 and a deletion of dipeptide(GQ) in subunit 1Dy12^*.Also,there were fifteen amino acid differences between subunits 1Dy12^* and 1Dy10,two hexapeptides(IGQGQQ) insertions and a deletion of dipeptide(GQ) in subunit 1Dy12^*.This showed that subunit 1Dy12^* is a novel HMW-GS,and it is in process of evaluating subunit 1Dy12^* to effect wheat processing quality.