Studies on Morphogenesis and Development of Arabidopsis thaliana Stomata Using Confocal Laser Scanning Microscope

The morphogenesis and development of lamina stomata in Arabidopsis thaliana was studied,usingconfocal laser scanning microscope/Uhe results showed that all types of cells,such as trichomes,subsidiary,guard and epidermal pavement cells,and their morphogenesis variation were observed clearly under UV laser(351 nm).During the formation of stomata,one protodermal cell produced a meristemoid and subsidiary cells by asymmetric division,followed by the formation of guard mother cell,which became two guard cells.Stomata were terminally differentiated.After the formation of stomata,guard cells could not emit fluorescence under UV laser(351 nm).So blue laser(488 nm)with staining by fluorescein was adopted and guard cells were seen clearly.It indicated that CLSM was a very novel tool to study the morphogenesis and development of lamina stomata.