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LI Xiu-Ling, HUANG Chang-Yan, SONG Qian, ZHOU Jin-Ye, WANG Xiao-Guo, QU Ting-Ting, HUANG Feng-Ling, BU Zhao-Yang. In vitro Asymbiotic Germination and Propagation of Paphiopedilum concolor (Lindl.) Pfitz.[J]. Plant Science Journal, 2016, 34(1): 127-134. DOI: 10.11913/PSJ.2095-0837.2016.10127
Citation: LI Xiu-Ling, HUANG Chang-Yan, SONG Qian, ZHOU Jin-Ye, WANG Xiao-Guo, QU Ting-Ting, HUANG Feng-Ling, BU Zhao-Yang. In vitro Asymbiotic Germination and Propagation of Paphiopedilum concolor (Lindl.) Pfitz.[J]. Plant Science Journal, 2016, 34(1): 127-134. DOI: 10.11913/PSJ.2095-0837.2016.10127
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In vitro Asymbiotic Germination and Propagation of Paphiopedilum concolor (Lindl.) Pfitz.

  • Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

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This work was supported by grants from the Guangxi Science and Technology Development Plan (Guangxi cooperation, 15104001-13) and the Basic Science Funds from Guangxi Academy of Agricultural Sciences (GXAAS, 2014YQ03 and 2015YT89).

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  • Received Date: August 31, 2015
  • Available Online: October 31, 2022
  • Published Date: February 27, 2016
    Graphical Abstract
    • Abstract
  • Different embryo-aged Paphiopedilum concolor seeds were collected and their structure, germination process, and effective regeneration conditions were investigated. Results showed that the seeds collected from 210-240 days after pollination (DAP) were optimum for culture in vitro, and the seed germination percentage of 210 DAP was the highest (77.79%). Furthermore, 1/4 MS (Murashige and Skoog culture medium) and 1/2 MS were both suitable for germination, and the addition of 100 mL/L coconut water (CW) and 1 g/L peptone had a positive effect on seed germination and protocorm differentiation. The addition of 1 g/L activated charcoal (AC) inhibited protocorm browning and death, while the addition of 2 g/L AC increased the browning and mortality rates. Banana juice and apple juice had a negative effect on seed germination and protocorm differentiation. After culturing of transferred differentiation protocorm on rooting medium for 120 days, the healthy robust seedlings were 3-5 cm tall and had 5-6 leaves.
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