Cloning of miR164b precursor from <i>Phyllostachys edulis</i> and analysis of its function in leaf morphogenesis

Wang Li-Li; Li Li-Chao; Sun Hua-Yu; Zhao Han-Sheng; Gao Zhi-Min

doi:10.11913/PSJ.2095-0837.2017.40551

Plant Science Journal > 2017 > 35(4): 551-557. > DOI: 10.11913/PSJ.2095-0837.2017.40551 CSTR: 32231.14.PSJ.2095-0837.2017.40551

Wang Li-Li, Li Li-Chao, Sun Hua-Yu, Zhao Han-Sheng, Gao Zhi-Min. Cloning of miR164b precursor from Phyllostachys edulis and analysis of its function in leaf morphogenesis[J]. Plant Science Journal, 2017, 35(4): 551-557. DOI: 10.11913/PSJ.2095-0837.2017.40551

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Cloning of miR164b precursor from Phyllostachys edulis and analysis of its function in leaf morphogenesis

1. International Center for Bamboo and Rattan Key Laboratory on the Science and Technology of Bamboo and Rattan, Beijing 100102, China;
2. Lianyungang Academy of Agricultural Sciences, Lianyungang, Jiangsu 222006, China

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This work was funded by a grant from the National Sci-Tech Support Plan of the Twelfth Five-year (2015BAD04B01).

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Received Date: December 19, 2016
Available Online: October 31, 2022
Published Date: August 27, 2017

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Abstract

Abstract

MicroRNA (miRNA) plays a crucial role in the regulation of plant morphogenesis by participating in a variety of physiological metabolic processes.The miR164 family is a group of plant-specific miRNAs,whose target genes are mainly the NAC transcription factors involved in the differentiation and development of apical meristems in stems and leaves,as well as in plant senesce.In this study,the precursor sequence of miR164b isolated from Phyllostachys edulis was 82 bp,which could form a stable stem-loop structure with the generated mature sequence on the 5'end of the arm.The mature sequences of the miR164 family were highly conserved.The expression vector with the target fragment harboring the miR164b precursor driven by CaMV 35S was constructed and transformed into Arabidopsis(Col-0).The phenotypes demonstrated that the transgenic Arabidopsis plants grew smaller and weaker,with fewer leaves,later flowering,and smoother leaf margins than those of Col-0.The real-time PCR results showed that the expression of miR164b was significantly increased (P < 0.01) and the expressions of CUC1 and CUC2 were significantly down-regulated (P < 0.01) in transgenic plants compared with Col-0.These results indicate that miR164b plays an important role in leaf morphogenesis by regulating the expression of CUC1 and CUC2.This study provides a useful reference for bamboo molecular breeding using miRNA strategies.
- Phyllostachys edulis,
- MiR164b,
- Precursor,
- Leaf morphogenesis

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References (30)

References

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Cloning of miR164b precursor from Phyllostachys edulis and analysis of its function in leaf morphogenesis

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