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Liu Hai-Tao, Wang Xi, Liu Ling, Gao Yan-Hua, Mu Dan-Ping, Chen Yu-Di. Effects of carboxylated multi-walled carbon nanotubes, mixed salt, and their combination on physiological characteristics of Oryza sativa seedlings[J]. Plant Science Journal, 2019, 37(4): 540-550. DOI: 10.11913/PSJ.2095-0837.2019.40540
Citation: Liu Hai-Tao, Wang Xi, Liu Ling, Gao Yan-Hua, Mu Dan-Ping, Chen Yu-Di. Effects of carboxylated multi-walled carbon nanotubes, mixed salt, and their combination on physiological characteristics of Oryza sativa seedlings[J]. Plant Science Journal, 2019, 37(4): 540-550. DOI: 10.11913/PSJ.2095-0837.2019.40540
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Effects of carboxylated multi-walled carbon nanotubes, mixed salt, and their combination on physiological characteristics of Oryza sativa seedlings

  • 1. School of Biological Engineering, Huainan Normal University, Huainan, Anhui 232038, China;

  • 2. Key Laboratory of Bioresource and Environmental Biotechnology of Anhui Higher Education Institutes, Huainan, Anhui 232038, China;

  • 3. Huainan Municipal Gardening Administration Bureau, Huainan, Anhui 232001, China

Funds: 

This work was supported by grants from the Natural Science Foundation of Anhui Province (1608085QC50), Program for Outstanding Young Talents in the University of Anhui Province (gxyq2019078), Domestic or Foreign Visiting and Studying Key Program of the Young and Middle-Aged Backbone Teachers for Higher Education Institutions from the Education Bureau of Anhui Province (gxfxZD2016202), Science and Technology Program of Huainan City(2018A29), and Science Research Foundation for Doctors of Huainan Normal University (51306).

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  • Received Date: January 07, 2019
  • Revised Date: January 20, 2019
  • Available Online: October 31, 2022
  • Published Date: August 27, 2019
    Graphical Abstract
    • Abstract
  • Rice (Oryza sativa L.) seedlings were hydroponically cultivated in different concentrations of carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) (0, 2.5, 5.0, and 10.0 mg/L), 50 mmol/L mixed salt (1NaCl:9Na2SO4:9NaHCO3:1Na2CO3), and mixed salt + MWCNTs-COOH for 10 d, respectively. Several physiological and biochemical parameters were then determined to investigate the phytotoxicity and ecotoxicological risks of MWCNTs-COOH and joint exposure with mixed salt on the seedling leaves. Results showed that reactive oxygen species (ROS), including superoxide radical (O2·-) and hydrogen peroxide (H2O2), were induced non-significantly by single MWCNTs-COOH treatment compared with the control. However, O2·- and H2O2 were overproduced in the mixed salt treatment group and in the combined treatment group. Mixed salt combined with MWCNTs-COOH aggravated the accumulation of O2·- and H2O2, with obvious concentration effects. As signaling molecules, ROS enhanced, at least to some extent, the total activities of antioxidant enzymes (i.e., SOD, CAT, POD, and APX) in the different treatment groups. The contents of chlorophyll-a and carotene somewhat increased under low concentration combined treatment compared with mixed salt treatment. After combination with mixed salt, the synthesis of soluble sugar and proline were inhibited, whereas the relative electrical conductivity and production of malondialdehyde (MDA) were significantly enhanced. The increased activity of antioxidant enzymes and production of chlorophyll-a and carotene are likely crucial defense mechanisms, which are beneficial for the alleviation of oxidative stress and damage, as well as for the maintenance of photosynthetic electron transport and thermal dissipation of excessive light energy in O. sativa seedlings. This study demonstrated that single MWCNTs-COOH treatment caused a certain level of oxidative stress and defense response in the leaves of O. sativa seedlings; furthermore, combined MWCNTs-COOH and mixed salt treatment aggravated oxidative stress and damage.
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