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Ji Yu-Lu, Yang Wei, Li Han, Cao Hua, Lu Lin, Tian Min, Sun Dan, Li Dong-Hui. Study on chloroplast ultrastructure, photosynthetic pigments, and chlorophyll fluorescence characteristics of leaf color mutants in Dendrobium officinale Kimura et Migo[J]. Plant Science Journal, 2020, 38(2): 260-268. DOI: 10.11913/PSJ.2095-0837.2020.20260
Citation: Ji Yu-Lu, Yang Wei, Li Han, Cao Hua, Lu Lin, Tian Min, Sun Dan, Li Dong-Hui. Study on chloroplast ultrastructure, photosynthetic pigments, and chlorophyll fluorescence characteristics of leaf color mutants in Dendrobium officinale Kimura et Migo[J]. Plant Science Journal, 2020, 38(2): 260-268. DOI: 10.11913/PSJ.2095-0837.2020.20260
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Study on chloroplast ultrastructure, photosynthetic pigments, and chlorophyll fluorescence characteristics of leaf color mutants in Dendrobium officinale Kimura et Migo

  • 1. College of Landscape Architecture, Yunnan Agricultural University, Kunming 650201, China;

  • 2. Institute of Flowers, Yunnan Academy of Agricultural Sciences, National Research Center for Ornamental Horticulture Engineering, Kunming 650100, China

Funds: 

This work was supported by grants from the Key R & D Plan of the Science and Technology Department of Yunnan Province (2018IB012), Major Science and Technology Special Plan of Yunnan Province (2017ZF016), Youth Project of Yunnan Provincial Department of Science and Technology (2017FD199), and Yunnan Agricultural University Student Science and Technology Innovation and Entrepreneurship Action Fund Project (2019ZKX078).

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  • Received Date: July 21, 2019
  • Revised Date: September 27, 2019
  • Available Online: October 31, 2022
  • Published Date: April 27, 2020
    Graphical Abstract
    • Abstract
  • The naturally mutated white-green chimera mutant (‘TP-MA’) and space-mutated green-yellow chimera mutant ('TP-MG’) of Dendrobium officinale (‘TP35’) were used as research materials. We investigated changes in chloroplast ultrastructure, photosynthetic pigment content, and chlorophyll fluorescence kinetic parameters of leaves after treatment with different light intensities (0, 50, 100 μmol·m-2·s-1), and clarified the differences in photo-synthetic characteristics between leaf color mutants and normal plants. Results showed that the chloroplast morphology of ‘TP-MA’ and ‘TP-MG’ both had a certain degree of deletion. The chloroplasts were unevenly distributed and irregular, and the basal layer structure was incomplete and loosely arranged, which was basically consistent with phenotypic traits. The photosynthetic pigment content and chlorophyll fluorescence parameters Fv/Fm， ΦPSⅡ and Fv'/Fm' of ‘TP-MA’ were significantly lower than those of ‘TP35’, but the chlorophyll quenching coefficients (NPQ and qP) were relatively higher. The photosynthetic pigment content and chlorophyll fluorescence parameters of ‘TP-MG’ were lower than those of ‘TP35’, but the differences were not significant, and it exhibited a certain adaptability to strong illumination (100 μmol·m-2·s-1). Thus, results showed that under different light intensities, the chloroplast structure and photosynthetic physiological indices of the D. officinale mutants changed to varying degrees and had certain effects on the chlorophyll content and fluorescence parameters of the plants. Too low or too high light intensity was not conducive to the normal growth of the plants.
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    • References (35)
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