Comparison of floral nectar extraction, preservation, and detection methods

Lun Han-Ning; Ye Zhong-Ming; Yang Chun-Feng

doi:10.11913/PSJ.2095-0837.2021.20201

Plant Science Journal > 2021 > 39(2): 201-207. > DOI: 10.11913/PSJ.2095-0837.2021.20201 CSTR: 32231.14.PSJ.2095-0837.2021.20201

Lun Han-Ning, Ye Zhong-Ming, Yang Chun-Feng. Comparison of floral nectar extraction, preservation, and detection methods[J]. Plant Science Journal, 2021, 39(2): 201-207. DOI: 10.11913/PSJ.2095-0837.2021.20201

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Comparison of floral nectar extraction, preservation, and detection methods

1. Research Center for Ecology and Environment of Qinghai-Tibetan Plateau, Tibet University, Lhasa 850000, China;
2. College of Science, Tibet University, Lhasa 850000, China;
3. Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China

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This work was supported by grants from the National Natural Science Foundation of China (31970253, 31800194).

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Received Date: January 04, 2021
Revised Date: January 20, 2021
Available Online: October 31, 2022
Published Date: April 27, 2021

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Abstract

Abstract

Nectar is rich in organic compounds and microelements. However, nectar volume and chemical composition can vary substantially among plant species. Thus, nectar extraction and storage methods may affect component analysis. Based on a systematic review of the current literature, we summarizes six common nectar sampling methods in the field, including micropipettes and microsyringes, microcapillary tubes, filter paper, solution dilution, centrifugation, and aspiration; six nectar storage methods, including refrigeration at 4 ℃, freezing at -20 ℃, ultra-low freezing at -80 ℃, liquid nitrogen storage at -198 ℃, antimicrobial preservation, and filter paper storage; and three nectar characteristic measurement methods, including handheld refractometry, colorimetry, and chromatography. We further discuss the advantages and disadvantages of these methods and their potential application for different plant species. Under appropriate conditions, the most commonly used methods for nectar collection, storage, and analysis are microcapillary tubes, ultra-low freezing at -80 ℃, and chromatography.
- Nectar,
- Nectar sampling,
- Nectar storage,
- Nectar measurement,
- Nectar composition

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References (39)

References

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