Accumulation regularity of polysaccharides and lignin and identification of lignin monomer types in stems of <i>Dendrobium</i> <i>officinale</i> Kimura et Migo

Wang Jian; Zhen Cai-Yun; Wei Lian-Ping; Chang Yan; Cai Yong-Ping; Jin Qing

doi:10.11913/PSJ.2095-0837.2021.30306

Plant Science Journal > 2021 > 39(3): 306-315. > DOI: 10.11913/PSJ.2095-0837.2021.30306 CSTR: 32231.14.PSJ.2095-0837.2021.30306

Wang Jian, Zhen Cai-Yun, Wei Lian-Ping, Chang Yan, Cai Yong-Ping, Jin Qing. Accumulation regularity of polysaccharides and lignin and identification of lignin monomer types in stems of Dendrobium officinale Kimura et Migo[J]. Plant Science Journal, 2021, 39(3): 306-315. DOI: 10.11913/PSJ.2095-0837.2021.30306

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Accumulation regularity of polysaccharides and lignin and identification of lignin monomer types in stems of Dendrobium officinale Kimura et Migo

College of Life Science, Anhui Agricultural University, Hefei 230036, China

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This work was supported by grants from the Universities Natural Science Research Project of Anhui (KJ2016A224), University Synergy Innovation Program of Anhui (GXXT-2019-043), Major Science and Technology Project in Anhui (17030701031), and Provincial University Student Innovation and Entrepreneurship Training Programs (201910364255).

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Received Date: November 02, 2020
Revised Date: January 11, 2021
Available Online: October 31, 2022
Published Date: June 27, 2021

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Abstract

Abstract

The stems of Dendrobium officinale Kimura et Migo in different years were used as study materials. The contents of polysaccharides, monosaccharides, and lignin were determined, and the relationships among them were analyzed by multiple linear regression analysis. The D. officinale stems were stained using saffron, fast green, and Mäule staining, and the lignin monomer types were determined by Fourier transform infrared (FTIR) and proton nuclear magnetic resonance (¹H-NMR) spectroscopy. As a result, lignin accumulation was significantly positively correlated with glucose content, and significantly negatively correlated with polysaccharide and mannose content and the mannose to glucose ratio. These results suggested that the accumulation of polysaccharides was influenced by the deposition of lignin. The xylem and sheath of the vascular bundles in the D. officinale stems contained lignin. With the increase in years, the thickness of the vascular thick-walled cells in the D. officinale stems increased, and the degree of development of the vascular bundle sheaths increased. Mäule staining and FTIR and ¹H-NMR spectrometry showed that the stems of D. officinale mainly contained guaiacyl lignins (G lignins) and syringyl lignins (S lignins), as well as trace hydroxyl-phenyl lignins (H lignins). G lignins were mainly distributed in the xylem, while S lignins were mainly distributed in the thick-walled cells. The S/G ratio in the D. officinale stems showed an upward trend with increasing years.
- Dendrobium officinale,
- Polysaccharides,
- Lignin,
- Lignin monomer types

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References (31)

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Accumulation regularity of polysaccharides and lignin and identification of lignin monomer types in stems of Dendrobium officinale Kimura et Migo

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Accumulation regularity of polysaccharides and lignin and identification of lignin monomer types in stems of Dendrobium officinale Kimura et Migo

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