Advance Search
Da Xiao-Wei, Sun Min, Wang Xin, Pang Hai-Long, Jia Ling-Yun, Feng Han-Qing. Detection of the effects of exogenous signal molecules on ATP levels in Arabidopsis thaliana(L.)Heynh. using fluorescence resonance energy transfer[J]. Plant Science Journal, 2022, 40(3): 390-397. DOI: 10.11913/PSJ.2095-0837.2022.30390
Citation: Da Xiao-Wei, Sun Min, Wang Xin, Pang Hai-Long, Jia Ling-Yun, Feng Han-Qing. Detection of the effects of exogenous signal molecules on ATP levels in Arabidopsis thaliana(L.)Heynh. using fluorescence resonance energy transfer[J]. Plant Science Journal, 2022, 40(3): 390-397. DOI: 10.11913/PSJ.2095-0837.2022.30390

Detection of the effects of exogenous signal molecules on ATP levels in Arabidopsis thaliana(L.)Heynh. using fluorescence resonance energy transfer

Funds: 

This work was supported by grants from the National Natural Science Foundation of China (31870246), Special Fund for Guiding Scientific and Technological Innovation Development of Gansu Province (2019ZX-05), Open Fund of New Rural Development Institute of Northwest Normal University, Key Research and Development Program of Gansu Province (18YF1NA051).

More Information
  • Received Date: October 11, 2021
  • Revised Date: December 17, 2021
  • Available Online: October 31, 2022
  • Published Date: June 27, 2022
  • We used an adenosine triphosphate (ATP) fluorescence protein-sensor (Ateam1.03-nD/nA) based on fluorescence resonance energy transfer (FRET) to analyze the effects of extracellular ATP, Ca2+, H2O2, and NO on ATP levels in the cytoplasm and chloroplasts of Arabidopsis thaliana seedlings. Results showed that ATP levels were higher in the cytoplasm than in the chloroplasts. Under treatment with different concentrations of the signal molecules, the FRET ratio of plastid-localized Ateam 1.03-nD/nA fluctuated non-significantly between 1.2 and 1.8. The FRET ratio of cytosol-localized Ateam1.03-nD/nA fluctuated non-significantly between 2.2 and 3.0. These results indicate that both cytoplasmic ATP and chloroplast ATP maintained relatively stable levels upon stimulation by signal molecules at different intensities.
  • [1]
    Stella N, Si LH, Ma Q, Gao Z. Optical aptasensors for adenosine triphosphate[J]. Theranostics, 2016, 6(10):1683-1702.
    [2]
    Valentina DC, Philippe F, Thomas N, Marlene E, Pao VC, et al. ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology[J]. eLife, 2017, 6(6):e26770.
    [3]
    Chivasa S, Tomé DF, Hamilton JM, Slabas AR. Proteomic analysis of extracellular ATP-regulated proteins identifies ATP synthase beta-subunit as a novel plant cell death regulator[J]. Mol Cell Proteomics, 2011, 10(3).
    [4]
    Manfredi G, Yang L, Gajewski CD, Mattiazzi M. Measurements of ATP in mammalian cells[J]. Methods, 2002, 26(4):317-326.
    [5]
    Khlyntseva SV, Bazel'YR, Vishnikin AB, Andruch V. Methods for the determination of adenosine triphosphate and other adenine nucleotides[J]. J Anal Chem, 2009, 64(7):657-673.
    [6]
    Corriden R, Insel PA. Basal release of ATP:an autocrine-paracrine mechanism for cell regulation[J]. Sci Signal, 2010, 3(104):re1.
    [7]
    Conley JM, Saranya R, Valentino SA, Mathew T, Marco I. Imaging extracellular ATP with a genetically-encoded, ratiometric fluorescent sensor[J]. PLoS One, 2017, 12(11):e0187481.
    [8]
    Lmamura H, Nhat K, Togawa H, Saito K, Lino R, et al. Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators[J]. Proc Natl Acad Sci USA, 2009, 106(37):15651-15656.
    [9]
    Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T. Reversible dimerization of aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators[J]. ACS Chem Biol, 2010, 5(2):215-222.
    [10]
    Logan DC, Leaver CJ. Mitochondria-targeted GFP highlights the heterogeneity of mitochondrial shape, size and movement within living plant cells[J]. J Exp Bot, 2000(346):865-871.
    [11]
    Ando T, Imamura H, Suzuki R, Aizaki H, Suzuki T. Visua-lization and measurement of ATP levels in living cells replicating hepatitis C virus genome RNA[J]. PLoS Pathog, 2012, 8(3):e1002561.
    [12]
    Lerchundi R, Huang N, Rose CR. Quantitative imaging of changes in astrocytic and neuronal adenosine triphosphate using two different variants of ateam[J]. Front Cell Neurosci, 2020, 14(14).
    [13]
    Tang D, Wei F, Khan A, Munsif F, Zhou R, et al. Degradation of mitochondrial structure and deficiency of complexⅠ[STXFZ] were associated with the transgenic CMS of rice[J]. Biol Res, 2021, 54(1):6.
    [14]
    Luo D, Hong X, Liu Z, Guo J, Liu YG. A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice[J]. Nat Genet, 2013, 45(5):573.
    [15]
    Lang T, Deng C, Yao J, Zhang H, Deng S. A salt-signaling network involving ethylene, extracellular ATP, hydrogen peroxide, and calcium mediates K+/Na+ homeostasis in Arabidopsis[J]. Int J Mol Sci, 2020,21(22):8683.
    [16]
    Kim SY, Sivaguru M, Stacey G. Extracellular ATP in plants. visualization, localization, and analysis of physiological significance in growth and signaling[J]. Plant Physiol, 2006,142(3):984-992.
    [17]
    Lu Y, Li N, Sun J, Hou P, Jing X, et al. Exogenous hydrogen peroxide, nitric oxide and calcium mediate root ion fluxes in two non-secretor mangrove species subjected to NaCl stress[J]. Tree Physiol, 2013,33(1):81-95.
    [18]
    Wei Z, Miao Q, Sun D, Yang G, Wu C, et al. The mitochondrial phosphate transporters modulate plant responses to salt stress via affecting ATP and gibberellin metabolism in Arabidopsis thaliana[J]. PLoS One, 2012, 7(8):e43530.
    [19]
    Noriyuki H, Vadim PK, Hiromi I, Hiroyuki N, Takeharu N. Changes in cytosolic ATP levels and intracellular morpho-logy during bacteria-induced hypersensitive cell death as revealed by real-time fluorescence microscopy imaging[J]. Plant Cell Physiol, 2012,53(10):1768.
    [20]
    Elsa M, Sun J, Wang L, Bhat MG, Mohammad-Sidik AB, et al. DORN1/P2K1 and purino-calcium signalling in plants; making waves with extracellular ATP[J]. Ann Bot, 2020,124(7):1227-1242.
    [21]
    Voon CP, Guan X, Sun Y, Sahu A, Lim BL. ATP compartmentation in plastids and cytosol of Arabidopsis thaliana revealed by fluorescent protein sensing[J]. Proc Natl Acad Sci USA, 2018, 115(45):E10778-E10787.
    [22]
    Tripathi D, Tanaka K. A crosstalk between extracellular ATP and jasmonate signaling pathways for plant defense[J]. Plant Signal Behav, 2018,13(5):511-523.
    [23]
    Choi J, Tanaka K, Cao Y, Yue Q, Jing Q, et al. Identification of a plant receptor for extracellular ATP[J]. Science, 2014, 343(6168):290-294.
    [24]
    Riveras E, Alvarez JM, Vidal EA, Oses C, Vega A, Gutiérrez RA. The calcium ion is a second messenger in the nitrate signaling pathway of Arabidopsis[J]. Plant Physiol, 2015, 169(2):1397-404.
    [25]
    Huang F, Luo J, Ning T, Cao W, Jin X, et al. Cytosolic and nucleosolic calcium signaling in response to osmotic and salt stresses are independent of each other in roots of Arabidopsis seedlings[J]. Front Plant, 2017, 8(8):1648.
    [26]
    Suzuki N. Alleviation by calcium of cadmium-induced root growth inhibition in Arabidopsis seedlings[J]. Plant Biotechnol, 2005, 22(1):19-25.
    [27]
    Clark G, Roux S. Role of Ca2+in mediating plant responses to extracellular ATP and ADP[J]. Int J Mol Sci, 2018, 19(11):3590.
    [28]
    Hieno A, Naznin HA, Inaba-Hasegawa K, Yokogawa T, Hayami N, et al. Transcriptome analysis and identification of a transcriptional regulatory network in the response to H2O2[J]. Plant Physiol, 2019,180(3):1629-1646.
    [29]
    Ara N, Nakkanong K, Yang JH, Hu ZY, Zhang MF. Dissecting the heat stress-induced alterations in the leaf ultrastructure and some antioxidant network components in interspecific (Cucurbita maxima×Cucurbita moschata) inbred line of squash'Maxchata'as to its parents posses-sing variable heat toleranc[J]. Plant Growth Regul, 2015,76(3):289-301.
    [30]
    Jagadis GK, Aprajita K, Igor FS, Fernie AR, Igamberdiev AU. Interaction of nitric oxide with the components of the plant mitochondrial electron transport chain[J]. J Exp Bot, 2018,69(14):14.
    [31]
    Tiwari BS, Belenghi B, Levine A. Oxidative stress increased respiration and generation of reactive oxygen species, resulting in ATP depletion, opening of mitochondrial permeability transition, and programmed cell death[J]. Plant Physiol. 2002,128(4):1271-1281.
    [32]
    Fuente IMDL, Cortés JM, Valero E, Desroches M, Rodrigues S, et al. On the dynamics of the adenylate energy system:homeorhesis vs homeostasis[J]. PLoS One, 2014, 9(10):e108676.
  • Related Articles

    [1]Zhang Wanlu, Ding Yong. Regulation of hypocotyl elongation in Arabidopsis thaliana (L.) Heynh. by U2BL[J]. Plant Science Journal, 2024, 42(1): 66-74. DOI: 10.11913/PSJ.2095-0837.23065
    [2]Li Rui, Lu Ming-Zhu, Liu Nian, Xiong Ya-Cen, Li Jing. Biological effects of carbon quantum dots on model plant Arabidopsis thaliana[J]. Plant Science Journal, 2019, 37(2): 240-250. DOI: 10.11913/PSJ.2095-0837.2019.20240
    [3]Shu Huang-Ying, Hao Yuan-Yuan, Cai Qing-Ze, Wang Zhen, Zhu Guo-Peng, Cheng Shan-Han, Zhou Yuan, Wang Zhi-Wei. Recent research progress on the molecular regulation of flowering time in Arabidopsis thaliana[J]. Plant Science Journal, 2017, 35(4): 603-608. DOI: 10.11913/PSJ.2095-0837.2017.40603
    [4]CHEN Dan, PENG Xiong-Bo. DEAD-box: Function on Growth and Development in Arabidopsis thaliana[J]. Plant Science Journal, 2016, 34(6): 941-948. DOI: 10.11913/PSJ.2095-0837.2016.60941
    [5]WEI Lei, FEI Zhen-Jiang. Mitochondrial RNA Editing of ATPase atp6 Gene Transcripts of Yunnan Purple Rice(Oryza sativa L.)[J]. Plant Science Journal, 2010, 28(3): 251-256. DOI: 10.3724/SP.J.1142.2010.30251
    [6]ZHANG Bi-Yu, LI Ling, ZHENG Si-Chun, FENG Qi-Li. The Cold-resistance and Ultra Structure of Leaf Cells in CfGST Transgenic Arabidopsis thaliana[J]. Plant Science Journal, 2009, 27(3): 246-249.
    [7]CUI Yong-Lan, WANG Peng-Cheng. Study on Subcellular Localization of Two Expressed Proteins in Arabidopsis thaliana[J]. Plant Science Journal, 2009, 27(2): 216-220.
    [8]WEI Lei, YAN Zhi-Xiang, YU Jin-Hong, DING Yi. Mitochondrial RNA Editing of ATPase atp9 Gene Transcripts of Yunnan Purple Rice(Oryza sativa L.)[J]. Plant Science Journal, 2008, 26(6): 567-572.
    [9]ZHOU Fen, ZENG Chang-Li, WANG Jian-Bo. Influence of Calcium on Alleviating NaCl-Induced Injury Effects in Arabidopsis thaliana Seedlings[J]. Plant Science Journal, 2004, 22(2): 179-182.
    [10]Li Meiru, Du Yanru, Guo Libo. PLASMALEMMA ATPase OF HYPOCOTYL OF PEANUT SEEDLING AND ITS REACTION TO LOW TEMPERATUER STRESS[J]. Plant Science Journal, 1999, 17(2): 110-114.

Catalog

    Article views (566) PDF downloads (284) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return