Study on the combined toxic effects of benzotriazole and cadmium on growth of rice seedlings

Rong Hong; Wang Cheng-Run; Tao Yu-Qing

doi:10.11913/PSJ.2095-0837.2022.50688

Plant Science Journal > 2022 > 40(5): 688-694. > DOI: 10.11913/PSJ.2095-0837.2022.50688 CSTR: 32231.14.PSJ.2095-0837.2022.50688

Rong Hong, Wang Cheng-Run, Tao Yu-Qing. Study on the combined toxic effects of benzotriazole and cadmium on growth of rice seedlings[J]. Plant Science Journal, 2022, 40(5): 688-694. DOI: 10.11913/PSJ.2095-0837.2022.50688

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Study on the combined toxic effects of benzotriazole and cadmium on growth of rice seedlings

School of Biological Engineering, Huainan Normal University, Huainan, Anhui, 232000, China

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This work was supported by grants from the State Key Laboratory of Pollution Control and Resource Reuse of China (PCRRF19036) and Scientific Research Project of Huainan Normal University,China (2019XJZD02).

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Received Date: February 27, 2022
Revised Date: May 30, 2022
Available Online: December 08, 2022

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Abstract

Abstract

This study investigated the toxic effects of benzotriazole (BTA) (2.5, 5, and 10 mg/L) and its combination with cadmium (Cd) (5 μmol/L)on rice (Oryza sativa L.) seedling growth. Results showed that with increasing concentration, BTA significantly decreased chlorophyll content,inhibited rice seedling growth, decreased soluble sugar and starch content, enhanced soluble protein content, free amino acid content, and nitrate reductase (NR) activity, and inhibited glutamine synthase (GS) and glutamate synthase (GOGAT) activity compared with the control. Compared with Cd alone, BTA and Cd in combination facilitated chlorophyll degradation and suppressed rice seedling growth. In addition, 2.5 and 5 mg/L BTA combined with Cd decreased starch and soluble sugar content, while high-concentration BTA (10 mg/L) combined with Cd elevated starch and soluble sugar content. Joint exposure also enhanced free amino acid content and NR activity but reduced GOGAT activity. Different concentrations of BTA in combination with Cd also resulted in high enhancement and low suppression of GS activity. Thus, BTA either alone or in combination with Cd affected nitrogen and carbon metabolism in the leaves of rice seedlings, indicating that 2.5~10 mg/L BTA inhibited carbon and nitrogen metabolism. However, increasing BTA combined with Cd exhibited high promotion and low suppression of carbon and nitrogen metabolism to a certain extent.
- C/N metabolism,
- Soluble protein,
- Cadmium,
- Benzotriazole,
- Oryza sativa seedlings

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References (30)

References

[1]	Xing YS, Meng XS, Wang L, Zhang JJ, Wu ZJ, et al. Effects of benzotriazole on copper accumulation and toxicity in earthworm (Eisenia fetida)[J]. J Hazard Mater, 2018, 351:330-336.
[2]	Li WZ, Li J, Deng M, Pan YN, Zeng LX. Benzotriazoles and benzothiazoles prevail in indoor dust from an E-waste dismantling area in South China:elevated concentrations and implication for human exposure[J]. Sci Total Environ, 2020,723:1-8.
[3]	Wang Z, He M, Chen BB, Hu B. Azo-linked porous organic polymers/polydimethylsiloxane coated stir bar for extraction of benzotriazole ultraviolet absorbers from environmental water and soil samples followed by high performance liquid chromatography-diode array detection[J]. J Chromatogr A, 2020, 1616:1-10.
[4]	李雯镜,李志彤,梁雪芳.苯并三唑类紫外稳定剂在环境中的检测、分布及其毒性效应[J].生态毒理学报, 2018, 13(1):89-105. Li WJ, Li ZT, Liang XF. Detection, distribution and toxicological effects of benzotriazole UV stabilizers[J]. Asian Journal of Ecotoxicology, 2018, 13(1):89-105.
[5]	Liu Z, Wang L, Wang C, Sun S, Qin L, et al. Effect of corrosion inhibitor benzotriazole on the uptake and translocation of Cd in rice (Oryza sativa L.) under different exposure conditions[J]. Chemosphere, 2017, 186:24-30.
[6]	王娇,赵爽,段鑫越,王予頔,秦莉,等.苯并三唑对镉在小油菜中的蓄积和毒性的影响[J].农业环境科学学报, 2020, 39(1):55-61. Wang J, Zhao S, Duan XY, Wang YD, Qin L, et al. Effects of benzotriazole on the uptake and toxicity of cadmium in rapes (Brassica campestris L.)[J]. Journal of Agro-Environment Science, 2020, 39(1):55-61.
[7]	Kim H, Kim B, Shin YJ, Kim J, Kim HJ, et al. Effect of benzotriazole on oxidative stress response and transcriptional gene expression in Oryzias latipes and Danio rerio embryo[J]. Comp Biochem and Phys C, 2022, 252:1-9.
[8]	Cheng CH, Ma HL, Deng YQ, Feng J, Jie YK, et al. Oxidative stress, cell cycle arrest, DNA damage and apoptosis in the mud crab (Scylla paramamosain) induced by cadmium exposure[J]. Chemosphere, 2021, 263:1-11.
[9]	He TT, Zhang T, Liu SB, Shi JC, Huang YS, et al. Toxicological effects benzotriazole to the marine scallop Chlamysnobilis:a 2-month exposure study[J]. Environ Sci Pollut R, 2019, 26(10):10306-10318.
[10]	Kolahi M, Mohajel Kazemi E, Yazdi M, Goldson-Barnaby A. Oxidative stress induced by cadmium in lettuce (Lactuca sativa Linn.):oxidative stress indicators and prediction of their genes[J]. Plant Physiol Bioch, 2020, 146:71-89.
[11]	Xing YS, Meng XS, Wang L, Zhang JJ, Wu ZJ, et al. Effects of benzotriazole on copper accumulation and toxi-city in earthworm (Eisenia fetida)[J]. J Hazard Mater, 2018, 351:330-336.
[12]	Duan ZH, Xing YS, Feng ZT, Zhang HY, Li CX, et al. Hepatotoxicity of benzotriazole and its effect on the cad-mium induced toxicity in zebrafish Danio rerio[J]. Environ Pollut, 2017, 224:706-713.
[13]	Zhang HJ, Xing YS, Ji ST, Pu J, Sun HW, et al. Benzotria-zole alleviates copper mediated lysosomal membrane damage and antioxidant defense system responses in earthworms (Eisenia fetida)[J]. Ecotox Environ Safe, 2020, 197:1-6.
[14]	刘嘉艺,廖杨子杰,李萱,文素芸,刘博文,等.不同氮处理和弱光下高羊茅生长及其碳氮代谢特征[J].草业科学, 2022, 39(1):96-103. Liu JY, Liao YZJ, Li X, Wen SY, Liu BW, et al. Effects of different nitrogen treatments on the growth and carbon-nitrogen metabolism of tall fescue under low-light stress[J]. Pratacultural Science, 2022, 39(1):96-103.
[15]	Yu JJ, Han JN, Wang RF, Li XX. Down-regulation of nitrogen/carbon metabolism coupledwith coordinative hormone modulation contributesto developmental inhibition of the maize ear under nitrogenlimitation[J]. Planta, 2016, 244(1):111-124.
[16]	Yoshida S, Forno DA, Cock JH, Gomez KA. Laboratory manual for physiological studies of rice[M]. 3rd ed. Mani-la:International Rice Research Institute, 1976:61-64.
[17]	Schelbert S, Aubry S, Burla B, Agne B, Kessler F, et al. Pheophytin pheophorbide hydrolase (pheophytinase) is involved in chlorophyll breakdown duringleaf senescence in Arabidopsis[J]. Plant Cell, 2009, 21(3):767-785.
[18]	Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem, 1976, 72:248-254.
[19]	Agüera E, Ruano D, Cabello P, de la Haba P. Impact of atmospheric CO₂ on growth, photosynthesis and nitrogen metabolism in cucumber (Cucumis sativus L.) plants[J]. Plant Physiol, 2006, 163(8):809-817.
[20]	Rosen H. A modified ninhydrin colorimetric analysis for amino acids[J]. Arch Biochem Biophys, 1957, 67:10-15.
[21]	李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社, 2000:1-278.
[22]	王小纯,熊淑萍,马新明,张娟娟,马志强.不同形态氮素对专用型小麦花后氮代谢关键酶活性及籽粒蛋白质含量的影响[J].生态学报, 2005, 25(4):802-807. Wang XC, Xiong SP, Ma XM, Zhang JJ, Ma ZQ. Effects of different nitrogen forms on key enzyme activity involved in nitrogen metabolism and grain protein content in specia-lity wheat cultivars[J]. Acta Ecologica Sinica, 2005, 25(4):802-807.
[23]	李聪聪,李乃光,吴正锋.高温干旱复合胁迫对花生幼苗生理指标的影响[J].中国油料作物学报, 2021, 43(5):906-913. Li CC, Li NG, Wu ZF. Effects of high temperature and drought combined stress on physiological indexes of seedlings ofdifferent peanut varieties[J]. Chinese Journal of Oil Crop Sciences, 2021, 43(5):906-913.
[24]	李妮亚,高俊凤,汪沛洪.小麦幼芽水分胁迫诱导蛋白的特征[J].植物生理学报, 1998, 24(1):65-71. Li NY, Gao JF, Wang PH. The characteristics of induced protein in shoots of wheat seedlings under water stress[J]. Acta Phytophysiologica Sinica, 1998, 24(1):65-71.
[25]	郑云普,王贺新,娄鑫,杨庆朋,徐明.木本植物非结构性碳水化合物变化及其影响因子研究进展[J].应用生态学报, 2014, 25(4):1188-1196. Zheng YP, Wang HX, Lou X, Yang QM, Xu M. Changes of non-structural carbohydrates and its impact factors in trees:a review[J]. Chinese Journal of Applied Ecology, 2014, 25(4):1188-1196.
[26]	张韫璐.镉胁迫对栽培大豆和野生大豆幼苗碳氮代谢影响的研究[D].沈阳:沈阳师范大学, 2018:9-45.
[27]	尹子旭,卢玉生,张美曼,姜小雨,严欣荣,等.光、氮及其互作对设施绿竹叶片碳氮代谢的影响[J].生态学杂志, 2020, 39(12):3979-3988. Yin ZX, Lu YS, Zhang MM, Jiang XY, Yan XR, et al.Interactive effects of light intensity and nitrogen supply on leaf carbon and nitrogen metabolism of Dendrocalamopsis oldhami[J]. Chinese Journal of Ecology, 2020, 39(12):3979-3988.
[28]	Silva IT, Abbaraju HKR, Fallis LP, Liu HJ, Lee M, Dhugga KS. Biochemical and genetic analyses of N metabolism in maize testcross seedlings:1. Leaves[J]. Theor Appl Genet, 2017, 130(7):1453-1466.
[29]	Paula MM, Ligia ML, Isabel MS, Helena GC, Julie VC.Expression of the plastid located glutamine synthetase of medicago truncatula. Accumulation of the precursor in root nodules reveals an in vivo control at the level of protein import into plastids[J]. Plant Physiol, 2003, 132(1):390-399.
[30]	凌丹丹,雒佳铭,刘晓英,储靖宇,徐志刚,等.不同光质及组合对番茄开花初期碳氮代谢及其关键酶活性的影响[J].南京农业大学学报, 2021,44(4):622-627. Ling DD, Luo JM, Liu XY, Chu JY, Xu ZG, et al. Effects of different light quality combinations on carbon andnitrogen metabolism and enzyme activities of tomato in earlyflowering period[J]. Journal of Nanjing Agricultural University, 2021, 44(4):622-627.

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