| Citation: |
Zhu K，Zuo QH，Yuan FH，Guan DX，Wu JB，Wang AZ，Zhang J. Effects of soil nitrogen addition on photosynthetic limitations in Fraxinus mandshurica Rupr. and Quercus mongolica Fish. ex Ledeb[J]. Plant Science Journal，2023，41（4）：502−512. DOI: 10.11913/PSJ.2095-0837.22206
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Based on natural nitrogen deposition in the field (23 kg·ha−1·year−1), this research employed low (LN, 23 kg·ha−1·year−1), moderate (MN, 46 kg·ha−1·year−1), and high (HN, 69 kg·ha−1·year−1) nitrogen levels to simulate natural nitrogen deposition, using no nitrogen addition used as a control (CK). The goal was to explore the physiological and ecological effects of excessive nitrogen deposition on two broad-leaved forest species, i.e., Manchurian ash (Fraxinus mandshurica Rupr.) and Mongolian oak (Quercus mongolica Fish. ex Ledeb). Results showed that 1) CO2 diffusional limitations (i.e., stomatal limitation, lsc, mesophyll limitation, lm) of both species decreased by more than 10% after nitrogen addition, then increased with increasing nitrogen supply, while biochemical limitation (lb) increased by more than 10% after nitrogen addition, then decreased with increasing nitrogen supply. 2) Both lsc and lm reached minimum values of 18.4% and 18.0% (Manchurian ash-August), 21.6% and 19.7% (Mongolian oak-July), and 21.6% and 20.1% (Mongolian oak-August), while lb reached a maximum value of 63.6% (Manchurian ash-August) and 59.7% and 58.3% (Mongolian oak-July and August) under MN treatment, indicating that soil nitrogen addition of 46 kg·ha-1·year-1 had the greatest photosynthesis-promoting effect. 3) The enhancement of plant photosynthetic capacity during soil nitrogen supply predominantly resulted from the weakening of CO2 diffusional limitations, in which stomatal conductance to CO2 (gsc, i.e., lsc) was the primary limiting factor affecting plant photosynthesis. 4) The three photosynthetic limitations (lsc, lm, and lb) did not show any significant differences between July and August, indicating that the primary photosynthetic role of lsc may lack seasonal variation. 5) Soil nitrogen addition within a certain content range did not significantly affect the water use potential of plants.
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